遗传 ›› 1995, Vol. 17 ›› Issue (1): 4-6.

• 论文 • 上一篇    下一篇

限制性内切酶缓冲液诱导人和家兔染色体显带的研究

毛新1 ;李虹2 ;陈文元3 MAO Xin1;LI Hong2;CHEN Wen-Yuan3   

  1. 1.华西医科大学精神科遗传室, 成都 610041 2.华西医科大学医学生物学教研室,成都 610041 3.四川大学生物系,成都 610064 1.Division of Medical Genetics,Department of Psychiatry,West China University of Medical Sciences,Chengdu 610041 2.Department of Medical Biology,West China University of Medical Science,Chendu 610041 3.Department of Biology,Sichuan University,Chengdu 610064
  • 收稿日期:1900-01-01 出版日期:1995-02-10 发布日期:1995-02-10

Study on Human and Rabbit Chromosome Banding by Restriction Endonucleases and Their Buffers

  • Received:1900-01-01 Online:1995-02-10 Published:1995-02-10

摘要: 用限制性内切酶AluⅠ、缓冲液或空白处理人染色体标本,发现AluⅠ及缓冲液均能诱导出类C和G带,其中缓冲液诱导的类C带频率虽低于酶原液,但明显高于空白对照; 而G带的诱导率无明显差异。此外,用限制性内切酶H ae Ⅲ和 HinfⅠ及其缓冲液处理中国小型白兔外周血染色体标本,也诱导出了相应的类C和G带。
Abstract:C-and G-bands were induced in human chromosome by both AluI and its buffer after treating the samples with AluI,buffer and blank control.The frequence of C-band induced by buffer was lower than that by AluI(P<0.05),but much higher than that by blank control(P<0.001).There was no significant difference among the frequencies of G-band induced by AluI,buffer and blank control(P>0.05).Also HaeIII,and their buffers were capable of inducing C-and G-bands on chromosome of China small-sized domestic rabbits.

关键词: 家兔, 染色体, 缓冲液, 人, 限制性内切酶, ES细胞, 表达, hGH基因, 3T3细胞, 转化

Key words: ES cells, Transfection, hGH gene, 3T3 cells