模式动物果蝇的基因调控前沿技术

doi:10.16288/j.yczz.21-347

遗传 ›› 2022, Vol. 44 ›› Issue (1): 3-14.doi: 10.16288/j.yczz.21-347

模式动物果蝇的基因调控前沿技术

韩玉婷(), 许博文, 李羽童, 卢心怡, 董习之, 邱雨浩, 车沁耘, 朱芮葆, 郑丽, 李孝宸, 司绪, 倪建泉()

清华大学医学院,基因表达与调控实验室,北京 100084

收稿日期:2021-10-07 修回日期:2021-12-21 出版日期:2022-01-20 发布日期:2022-01-04
通讯作者: 倪建泉 E-mail:hanyt19@mails.tsinghua.edu.cn;nijq@mail.tsinghua.edu.cn
作者简介:韩玉婷,在读博士研究生,研究方向：基因调控技术。E-mail: hanyt19@mails.tsinghua.edu.cn, 倪建泉课题组以果蝇为模式动物,开发遗传工具,研究表观遗传蛋白与信号通路在干细胞稳态中发挥的作用。课题组开发了包括基于CRISPR的基因编辑技术、基因定点激活技术和新一代转基因干扰技术在内的一系列果蝇遗传学新技术,实现在个体水平上特异调控细胞组织器官基因的表达。利用这些技术,建立转基因果蝇资源库,并进行了发育与干细胞相关的研究,主要成果包括确立组蛋白H1对干细胞的直接和间接调控作用;发现HP1c直接参与Notch信号的抑制,调控消化道干细胞稳态;确定了3个组蛋白乙酰化酶在果蝇眼睛发育中的协同作用及机制。相关研究成果发表在PNAS、Nature Communications、EMBO Reports 等期刊上。现作为课题组长,参与科技部支撑计划和发育代谢重大研发计划等。
基金资助:
国家自然科学基金项目编号(20181300988);国家自然科学基金项目编号(20201300797);科技部国家重点研发计划资助编号(2016YFE0113700)

The cutting edge of gene regulation approaches in model organism Drosophila

Han Yuting(), Xu Bowen, Li Yutong, Lu Xinyi, Dong Xizhi, Qiu Yuhao, Che Qinyun, Zhu Ruibao, Zheng Li, Li Xiaochen, Si Xu, Ni Jianquan()

Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China

Received:2021-10-07 Revised:2021-12-21 Online:2022-01-20 Published:2022-01-04
Contact: Ni Jianquan E-mail:hanyt19@mails.tsinghua.edu.cn;nijq@mail.tsinghua.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China Nos(20181300988);Supported by the National Natural Science Foundation of China Nos(20201300797);the National Key Technology Research and Development Program of the Ministry of Science and Technology of the People’s Republic of China No(2016YFE0113700)

摘要/Abstract

摘要：

转基因调控技术在生命医学研究中扮演了重要的角色,是探究个体发育和致病机制的必备工具。常用的转基因调控技术包括基因突变、基因干扰和基因转录激活等。果蝇由于具有基因的保守性、遗传工具的多样性以及不受伦理限制等优势,成为生命科学研究中经典模式动物之一,并由此开发出了多种时间和组织特异性的基因调控工具。本文主要介绍了目前在果蝇中常用的基因调控技术,包括CRISPR/Cas9介导的基因突变系统、基于miRNA的新一代转基因干扰系统以及基于CRISPR/dCas9的转录激活(flySAM)系统,希望通过对这几种系统设计原理、操作过程、相关的技术工具及相关资源品系的介绍,提升人们对这些前沿的果蝇遗传学基因表达调控技术及构建的相关果蝇资源品系重要性认识,进而推动生命医学研究发展。

关键词: 果蝇, 基因调控, CRISPR/Cas9, 基因编辑, 转基因RNAi, CRISPRa

Abstract:

Transgenic gene regulatory techniques play a critical role in biomedical fields since they are essential for researchers to study the molecular mechanisms of development and diseases. The currently prevalent transgenic techniques include specific gene mutation, transgenic RNAi and targeting activation, etc. Specifically, various gene regulatory tools have been developed to temporarily and spatially modulate genes in Drosophila melanogaster, which is a typical model organism with evolutionally conserved genome and without ethical issues in scientific research. Here, we introduce several gene regulatory techniques commonly used in Drosophila, including the principle, procedure, toolbox and related transgenic resource of CRISPR/Cas9-triggered heritable gene mutation system, next-generation transgenic RNAi and CRISPR/dCas9-based transcriptional activation (flySAM) approach. We wish to take this opportunity to raise the awareness of the importance of transgenic gene regulatory techniques development and related resource construction and thus to promote biomedical research progression.

Key words: Drosophila, gene regulation, CRISPR/Cas9, gene editing, transgenic RNAi, CRISPRa

韩玉婷, 许博文, 李羽童, 卢心怡, 董习之, 邱雨浩, 车沁耘, 朱芮葆, 郑丽, 李孝宸, 司绪, 倪建泉. 模式动物果蝇的基因调控前沿技术[J]. 遗传, 2022, 44(1): 3-14.

Han Yuting, Xu Bowen, Li Yutong, Lu Xinyi, Dong Xizhi, Qiu Yuhao, Che Qinyun, Zhu Ruibao, Zheng Li, Li Xiaochen, Si Xu, Ni Jianquan. The cutting edge of gene regulation approaches in model organism Drosophila[J]. Hereditas(Beijing), 2022, 44(1): 3-14.

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果蝇食物	混合玉米面（济南佳硕生物技术有限公司）、红糖（网山）、白砂糖（龙二）、酵母（安琪）、琼脂（科百奥）、乙醇（沪试）、丙酸（沪试）、抗生素（Amresco）
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果蝇管	绍兴上虞八达通信电器有限公司
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模式动物果蝇的基因调控前沿技术

The cutting edge of gene regulation approaches in model organism Drosophila

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