遗传 ›› 2003, Vol. 25 ›› Issue (4): 425-427.
王邦俊1;2;王强1;张志刚2;张劲松2;李学刚1 WANG Bang-Jun1;2;WANG Qiang1;ZHANG Zhi-Gang2;ZHANG Jin-Song2;LI Xue-Gang1
摘要: 利用抗病基因保守序列筛选大豆cDNA文库,获得一抗病基因同源cDNA片段,命名为KR3-1。根据KR3-1设计两个基因特异引物(GSP 和 NGSP),分别与通用引物(UPM)和巢式通用引物(NUP)共同扩增,成功地克隆到了该基因的5′末端序列。该扩增片段长447 bp,与已知序列重叠部分为129 bp。
Abstract:Based on part of a known partial cDNA sequence of a disease resistance gene homolog,KR3-1,obtained by screening a cDNA library from soybean,5′-RACE-PCR was carried out with gene specific primers and universal primers.After the nested PCR reaction,an amplified fragment of 447 bp in length which overlapped the known KR3-1 sequence by 129 bp was obtained subsequently.Thus,a 5′ cDNA end of KR3 was successfully cloned.