遗传 ›› 2003, Vol. 25 ›› Issue (4): 401-408.
陈国宏1;刘莉1;张学余2;李碧春1;吴信生1;徐琪1 CHEN Guo-Hong1;LIU Li1;ZHANG Xue-Yu2;LI Bi-Chun1;WU Xin-Sheng1;XU Qi1
摘要： 采用外周血淋巴细胞培养--空气干燥法，对泰和乌骨鸡染色体核型和带型进行了研究。结果表明：泰和乌骨鸡体细胞染色体数目2n=78,染色体基本臂数AF=90，1、9号染色体及Z、W性染色体为中央着丝粒（m）染色体，2、4、7号染色体为亚中央着丝粒（sm）染色体，3、6、8、10号染色体为端着丝粒（t）染色体。G带研究表明：前10对大型染色体可分为29个区，190条带。C带处理发现，所有母鸡分裂相中W性染色体都出现C带并整条深染。Ag-NORs研究发现：Ag-NORs常定位于1、2号常染色体短臂和Z性染色体短臂端部；Ag-NORs数目分布范围为1～6；平均每个细胞的Ag-NORs数在雌、雄鸡中分别为2.94和2.96。
Abstract:This study made the chromosome slides of Taihe Silkies by the peripheral blood lymphocyte culture-drying method,and analyzed Taihe Silkies karyotype and band pattern.The results are as follow:The diploid chromosome number of Taihe Silkies was 2n=78,the basic number of chromosome arms was AF=90 and the sex chromosome type was ZZ(♂)/ZW(♀).According to the measured relative length,arm ratio and centromeric index,the first 10 pairs of macro-chromosomes are described as follows:No.1 ，9 and Z,W chromosomes were metacentrics,No.2,4,7 were submetacentrics,and No.3,6,8,10 were telocentrics.Studies on Taihe Silkies′ G-band showed that the first 10 pairs of macro-chromosomes can be divided into 29 zones and 190 bands.Being treated by C-banding technique,a totally dark-stained and easily indentified W-chromosome always showed up in the female metaphase configurations.Ag-NORs were located in the short arms′ telomere of No.1,2 euchromosomes and Z sexchromosome,the Ag-NORs number varied from 1-6.
-NORs
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