遗传 ›› 2004, Vol. 26 ›› Issue (2): 181-185.

• 论文 • 上一篇    下一篇

水稻胞质核糖体蛋白基因OsRPS7的克隆与序列分析

顾志敏;王建飞;黄 骥;张红生GU Zhi-Min; WANG Jian-Fei; HUANG Ji; ZHANG Hong-Sheng   

  1. 南京农业大学 作物遗传与种质创新国家重点实验室,江苏 南京210095 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-03-28 发布日期:2004-03-28

Molecular Cloning and Sequece Analyzing of Cytoplasmic Ribosomal Protein Gene OsRPS7 From Rice ( Oryza sativa)

  • Received:1900-01-01 Revised:1900-01-01 Online:2004-03-28 Published:2004-03-28

摘要: 以已公布的黑麦胞质核糖体蛋白基因ScRPS7的cDNA序列为信息探针,在中国华大水稻基因组数据库中搜索与之高度同源的基因组重叠群。采用计算机拼接和RT-PCR方法克隆了水稻胞质核糖体蛋白基因的全长cDNA序列,命名为OsRPS7。该cDNA序列全长919bp,编码192个氨基酸;其与黑麦、拟南芥和芸薹的S7核糖体蛋白的氨基酸一致率分别为88%、72%和72%。对OsRPS7 的基因组结构和基因的功能进行了分析和预测。Abstract:Using the cDNA of rye cytoplasmic ribosomal protein ScRPS7 as a query probe, a highly homologous rice genomic contig was obtained from Huada rice genome database. The full-length cDNA sequence of rice cytoplasmic ribosomal protein S7 was assembled by informatics based on the contig. Furthermore, with the two primers designed according to this assembled cDNA, the full-length cDNA of rice ribosomal protein was cloned by RT-PCR and named as OsRPS7. The cDNA was 919bp in length and contained a complete Open Reading Frame (ORF) of 576bp, encoding a protein of 192 amino acid residues. The deduced amino acids of OsRPS7 showed 88%、72% and 72% identity with those from Secale cereale、Arabidopsis thaliana and Brassica oleracea, respectively. The genome structure of OsRPS7 was analyzed, and its function was predicted in this paper.

关键词: 基因克隆, 基因结构
Key words,
rice, 核糖体蛋白S7, 水稻