遗传 ›› 2007, Vol. 29 ›› Issue (8): 907-914.doi: 10.1360/yc-007-0907

• 综述 • 上一篇    下一篇

RNA扩增的研究进展

李轶女1, 胡英考2, 张志芳1, 沈桂芳1   

  1. 1.中国农业科学院生物技术研究所, 北京 100081;
    2.首都师范大学生命科学学院, 北京 100037

  • 收稿日期:2007-01-08 修回日期:2007-02-26 出版日期:2007-08-10 发布日期:2007-08-10
  • 通讯作者: 胡英考

Progress of RNA amplification

LI Yi-Nu1, HU Ying-Kao2, ZHANG Zhi-Fang1, SHEN Gui-Fang1

  

  1. 1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Life Science, Capital Normal University, Beijing 100037, China
  • Received:2007-01-08 Revised:2007-02-26 Online:2007-08-10 Published:2007-08-10
  • Contact: Ying-Kao HU

摘要: RNA扩增是伴随着基因芯片技术的应用而发展起来的一项技术,但不仅仅应用于芯片杂交。本文对RNA扩增的技术方法进行了综述,重点介绍了mRNA的线性扩增、指数扩增以及两者相结合的扩增方法以及优缺点,对microRNA的扩增方法也作了介绍,并对将来的发展作了展望。

关键词: 基因芯片, 小RNA, 聚合酶链式反应, 体外转录, RNA扩增

Abstract:

The technology of RNA amplification is developing with the application of gene chip and it is not only adapted for chip hybridization, but also applied in other purposes as well. The methodologies of RNA amplification were reviewed, focused on linear, exponential and linear plus exponential amplifications. The amplification of microRNA was also introduced and a prospect of RNA amplification was made.