基因芯片研究植物逆境基因表达新进展

doi:10.3724/SP.J.1005.2009.01192

遗传 ›› 2009, Vol. 31 ›› Issue (12): 1192-1204.doi: 10.3724/SP.J.1005.2009.01192

基因芯片研究植物逆境基因表达新进展

林海建, 张志明, 沈亚欧, 高世斌, 潘光堂

四川农业大学玉米研究所, 教育部作物基因资源与遗传改良重点实验室, 雅安625014

收稿日期:2009-04-12 修回日期:2009-05-17 出版日期:2009-12-10 发布日期:2009-12-10
通讯作者: 潘光堂 E-mail:pangt1956@yahoo.com.cn
基金资助:
国家高技术研究发展计划项目(863计划)(编号：2007AA10Z172), 国家自然科学基金项目(编号：30700506)和国家科技支撑计划项目 (编号：2006BAD01A03)资助

Advances of microarray analysis on plant gene expression under en-vironmental stresses

LIN Hai-Jian, ZHANG Zhi-Ming, SHEN Ya-Ou, GAO Shi-Bin, PAN Guang-Tang

Institute of Maize Research, Sichuan Agricultural University, Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Ya'an 625014, China

Received:2009-04-12 Revised:2009-05-17 Online:2009-12-10 Published:2009-12-10
Contact: PAN Guang-Tang E-mail:pangt1956@yahoo.com.cn

摘要/Abstract

摘要：

逆境胁迫影响植物的正常生长, 导致作物减产, 甚至绝收。提高作物的抗逆性一直是作物遗传育种学家追求的目标, 大量研究也正试图揭示这一复杂的生物学机制。传统的从生理生化水平到单一基因的研究都难以揭示植物复杂的抗逆机制, 而基因芯片(Gene chip)的应用使得这一目标成为了可能, 基因芯片从整个转录水平入手, 能够揭示大量基因的表达和调控情况, 同时结合蛋白质组学和代谢组学的研究方法, 将基因定位于代谢途径的某个位置, 寻找逆境胁迫响应的关键基因, 完善植物逆境胁迫响应的分子网络, 为今后利用生物技术手段提高作物抗逆境胁迫能力提供依据。文章主要对近年来基因芯片在植物逆境胁迫基因表达研究中的进展进行了综述。

关键词: 逆境胁迫, 基因芯片, 胁迫响应, 表达调控

Abstract:

Different stressed conditions impair plant growth and further, cause great loss of crop yield and even lead to lose production completely. Increasing resistance/tolerance of crops under stressed conditions is a major goal of numerous plant breeders, and many elegant works are focusing on this area to uncover these complicated mechanisms underlying it. However, the traditional strategies including physiological and biochemical methods, as well as studies on a few genes, can not well understand the overall biological mechanism. Microarray analysis opens a door to uncover these cryptic mechanisms, and has the ability of detecting gene transcription and regulation at genomic level in different plant tissues. And works in association with related methods of proteomics and metabolomics. Therefore, it is possible to locate genes in certain key metabolism pathways. Through these procedures, it is also possible to look for critical genes in the pathway and to well understand the molecular mechanism of resistance/tolerance. These results can be as a guidance for increasing the re-sistance/tolerance of stressed conditions using biotechnology methods in future. This paper mainly focused on and dis-cussed the advances of microarray analysis of stressed conditions-related genes in plants.

Key words: biotic/abiotic stresses, gene chip, stress response, expression and regulation

林海建，张志明，沈亚欧，高世斌，潘光堂. 基因芯片研究植物逆境基因表达新进展[J]. 遗传, 2009, 31(12): 1192-1204.

LIN Hai-Jian, ZHANG Zhi-Meng, CHEN E-Ou, GAO Shi-Bin, BO Guang-Tang. Advances of microarray analysis on plant gene expression under en-vironmental stresses[J]. HEREDITAS, 2009, 31(12): 1192-1204.

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基因芯片研究植物逆境基因表达新进展

Advances of microarray analysis on plant gene expression under en-vironmental stresses

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