9个野生中国樱桃群体叶绿体DNA trnQ-rps16序列变异及其遗传结构分析

doi:10.3724/SP.J.1005.2012.01475

遗传 ›› 2012, Vol. 34 ›› Issue (11): 1475-1483.doi: 10.3724/SP.J.1005.2012.01475

9个野生中国樱桃群体叶绿体DNA trnQ-rps16序列变异及其遗传结构分析

陈涛¹, 王小蓉^1,2, 罗华³, 王春涛³, 张家志³, 罗明敏⁴

1. 四川农业大学园艺学院, 雅安 625014 2. 四川农业大学果蔬研究所, 成都 611130 3. 四川省石棉县农业局经济作物站, 石棉 625400 4. 四川省石棉县农业局植保站, 石棉 625400

收稿日期:2012-08-31 修回日期:2012-10-16 出版日期:2012-11-20 发布日期:2012-11-25
通讯作者: 王小蓉 E-mail:wangxrtj@163.com
基金资助:
四川省教育厅重点培育专项(编号：2011A005)资助

Chloroplast DNA trnQ-rps16 variation and genetic structure of nine wild Chinese cherry (Cerasus pseudocerasus Lindl.) populations

CHEN Tao¹, WANG Xiao-Rong^1,2, LUO Hua³, WANG Chun-Tao³, ZHANG Jia-Zhi³, LUO Ming-Min⁴

1. College of Horticulture, Sichuan Agricultural University, Ya’an 625014, China 2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China 3. Economic Crop Unit, Agricultural Bureau of Shimian, Shimian 625400, China 4. Plant Protection Unit, Agricultural Bureau of Shimian, Shimian 625400, China

Received:2012-08-31 Revised:2012-10-16 Online:2012-11-20 Published:2012-11-25
Contact: Xiao-Rong WANG E-mail:wangxrtj@163.com

摘要/Abstract

摘要： 中国樱桃(Cerasus pseudocerasus Lindl.)是我国古老的具有较高经济价值的栽培果树之一, 个别性状突出的野生中国樱桃是对现有栽培品种进行遗传改良的重要资源。四川野生中国樱桃资源丰富, 为了明确该地区野生中国樱桃群体的遗传多样性和遗传结构, 文章对9个野生中国樱桃群体(其中7个分布四川, 2个来自陕西和贵州)共145个个体的叶绿体基因间隔区trnQ-rps16序列进行了测定和分析。结果表明：9个群体145个个体的trnQ-rps16序列比对后共检测到13个多态位点, 占位点总数的1.87%, 其中3处碱基替换, 10处插入/缺失。9个群体总的遗传多样性水平较低(h=0.562, π=0.00184), 相对于其他地区的2个群体(h= 0.733; π= 0.00243), 四川的7个群体表现出更低的遗传多样性水平(h= 0.544; π= 0.00203), 且群体间的遗传多样性水平存在较大差异(h=0-0.708; π=0-0.00298), 其中北川桃龙群体最高(h=0.708, π=0.00298), 而峨眉群体最低(h=0.000, π=0.000)。群体内低的遗传多样性可能与群体的边缘性所产生的奠基者效应以及近期群体收缩和随机遗传漂变造成的瓶颈效应导致群体内遗传多样性丢失有关。此外, 9个群体遗传分化水平较低, 平均F_ST为0.21573。分析认为主要是由于野生中国樱桃较强的种子传播能力增加了群体间的基因流动而导致遗传分化不明显, 也可能与野生中国樱桃较长的世代周期有关。针对上述研究结果, 建议在资源保护中采取减少群体数目而加大群体内的个体数量的保护策略。

关键词: 野生中国樱桃, 绿体DNA, 列变异, 传多样性, 传结构

Abstract: Chinese cherry (Cerasus pseudocerasus Lindl.) is one of the most economically domestic fruit trees in China. The rich variation of wild Chinese cherry is the most important breeding resource for existing cultivars. In order to reveal the levels and distribution of genetic variation within wild Chinese cherry of Sichuan Province, China, where is rich in wild Chinese cherry, the sequence variation of chloroplast DNA trnQ-rps16 intergenic spacer was analyzed in 145 individuals of all nine existing populations (seven from Sichuan, two from Shanxi and Guizhou provinces) of China. The results showed that trnQ-rps16 sequence were aligned with 13 polymorphic sites (1.87%), including 3 substitutions and 10 indels in 145 individuals, which revealed a low level of genetic diversity (h= 0.562, π= 0.00184). Compared to other regions (h= 0.733, π= 0.00243), a rather lower genetic diversity (h= 0.544, π= 0.00203) was found in the populations from Sichuan, and a large scale of genetic diversity among the seven populations was detected (h= 0-0.708; π= 0-0.00298), ranging from EM (h=0.000, π=0.000) to TL (h=0.708, π=0.00298). The low genetic diversity of populations may be strongly affected by founder effect and bottleneck effect because of the marginal nature, recent reduction, and consequent genetic drift of these populations. In addition, a fairly low genetic differentiation (F_ST= 0.21573) was found among the studied populations. This suggest that gene flow seems to originate from pronounced seed dispersal abilities of the species and it may play a significant role in shaping such a genetic structure. The long generation cycle of the species may also contribute to this structure. Based on these findings, a conservational plan for sampling or preserving fewer populations but more individuals from each population for the species was proposed.

Key words: wild Chinese cherry (Cerasus pseudocerasus Lindl.), chloroplast DNA, sequence variation, genetic diversity, genetic structure

陈涛，王小蓉，罗华，王春涛，张家志，罗明敏. 9个野生中国樱桃群体叶绿体DNA trnQ-rps16序列变异及其遗传结构分析[J]. 遗传, 2012, 34(11): 1475-1483.

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9个野生中国樱桃群体叶绿体DNA trnQ-rps16序列变异及其遗传结构分析

Chloroplast DNA trnQ-rps16 variation and genetic structure of nine wild Chinese cherry (Cerasus pseudocerasus Lindl.) populations

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