遗传 ›› 2013, Vol. 35 ›› Issue (7): 867-874.doi: 10.3724/SP.J.1005.2013.00867

• 综述 • 上一篇    下一篇

植物基因组DNase I超敏感位点的研究进展

张韬, 杨足君   

  1. 电子科技大学生命科学与技术学院, 成都610054
  • 收稿日期:2012-12-20 修回日期:2013-02-04 出版日期:2013-07-20 发布日期:2013-07-25
  • 通讯作者: 杨足君 E-mail:yangzujun@uestc.edu.cn
  • 基金资助:

    国家自然科学基金项目(编号:31171542和31101143)和电子科技大学中央高校业务费项目资助

Progress on identification and analysis of DNase I hypersensitive sites in plant genomes

ZHANG Tao, YANG Zu-Jun   

  1. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
  • Received:2012-12-20 Revised:2013-02-04 Online:2013-07-20 Published:2013-07-25

摘要: 真核生物的基因表达与调控是一系列顺式作用元件(cis-acting element)与反式作用因子(trans-acting factor)相互作用的结果。顺式作用元件本身不编码蛋白, 只是基因组中一些特定的区域, 它们需与反式作用因子结合才能发挥其生理作用。目前已知, 顺式作用元件往往与染色质的结构密切相关, 能与反式作用因子结合发挥生物学功能的顺式作用元件, 通常存在于脱氧核糖核酸酶I超敏感位点 (DNase I hypersensitive site) 的内部或周围。随着越来越多的植物基因组测序的进展, 大规模高通量鉴定植物基因组的顺式作用元件正在受到关注, 通过鉴定脱氧核糖核酸酶I超敏感位点可以有效确定顺式作用元件的富集区域, 将对功能基因组学研究提供的数据支持。文章综述了近期高通量鉴定和分析植物基因组脱氧核糖核酸酶 I 超敏感位点研究进展。

关键词: 植物基因组, 基因表达调控, 脱氧核糖核酸酶I超敏感位点

Abstract: Eukaryotes’s gene expression and regulation relies on the interaction of their cis-acting elements and trans-acting factors. The cis-elements are specific DNA sequences in the genome and frequently located in the untranslated regions. The trans-acting factors are usually considered to be proteins that bind to the cis-acting sequences to regulate gene expression. It is well known that the cis-elements are always associated with DNase I hypersensitive site, which is a signature of open chromatin. The identification of all the functional cis-element using high-throughput method in plant genome has not been initiated in plant genomes. With the rapid achievement of genomics studies, an increasing number of plant genomes have been sequenced. Genome-wide identification of DNase I hypersensitive sites will be a considerably efficient method to locate cis-element in plants, which will provide a vital potential for further plant functional genomics. The present review is to reveal the recent progresses on identification and analysis of DNase I hypersensitive site in plant genomes.

Key words: plant genome, gene expression and regulation, DNase I hypersensitive site