遗传 ›› 1994, Vol. 16 ›› Issue (1): 31-34.

• 论文 • 上一篇    下一篇

大麦花药培养中海藻酸钠作用的初步探讨

戎均康 黄巧玲RONG Jun-Gang HUANG Qiao-Ling   

  1. (浙江农业大学农学系 杭州 310029)Department of Agronomy, Zhejiang Agricultural University hangzhou 310029
  • 收稿日期:1900-01-01 出版日期:1994-02-10 发布日期:1994-02-10

Preliminary Study on the Use of Sodium Alginate ib Barley (Hordeum vulgare L.) Anther Culture

  • Received:1900-01-01 Online:1994-02-10 Published:1994-02-10

摘要:

本试验以普通大麦(Hordeum vulgare L.)品种单核晚期的花药为材料,研究了他们在液体培养过程中海藻酸钠的作用。结果表明:在供试的4个海藻酸钠浓度(1、2、3、6%)中,以浓度为2-3%的效果最佳。当他们代替20-30%的Ficoll时,无论是用BAC3培养基,还是用MS培养基,培养基中加NAA,还是家2,4-D,都能诱导出愈伤组织,但以每升培养基加2mg NAA和1 mg 6-BA的效果最佳,在含这种激素的培养基上,一些基因型的出愈率相当高,供试基因型都在100%以上,而且有些花粉粒能直接发育成能正常发芽出苗的胚状体。Barley anthers at the late uninucleate stage were cultured in order to study the use of sodium alginate in liquidmedium.The results showed that, the effect of 2-3% (W/V) sodium alginate was best in 4 concentration studied (1%,2%,3%and 6%). When the 2-3% sodium alginate was substituted for 20-30% ficoll, the calli could be induced from anthers of all genotypes whether on BAC3 medium or MS medium and whether adding NAA or 2,4-Din medium.2mg/L NAA+1mg/L 6-BA added to medium could give better results and on the medium with these hormones the callus rate of all genotypes were above 100%. Some pollens could grow into embryoids directly.

关键词: 花药培养, Sodium alginate, 愈伤组织Hordeum vulgare L., 海藻酸钠, 大麦, 体细胞无性系变异, 广亲和性, 水稻, 隐性高秆

Key words: Wide compatibility, Recessive talliness, somaclonal variation, Rice