遗传 ›› 1997, Vol. 19 ›› Issue (2): 11-14.

• 论文 • 上一篇    下一篇

云南汉族人群D17S30位点扩增片段长度多态性A

欧炯文1; 邹浪萍2; 李建京1; 申滨1; 韩晓华1; 赵永和1 OU Jiong-Wen1;ZOU Lang-Ping2;LI Jiang-Jing1;SHEN Bin1;HAN Xiao-Hua1;ZHAO Yong-He1   

  1. 1.昆明医学院法医学系, 昆明 650031 2.云南省高级人民法院法医处,昆明 650021 1.Department of Forensic Medicine,Kunming Medical College,Kunming 650031 2.Division of Forensic Medicine,Yunnan Provincial Higher People’s Court,Kunming 650021
  • 收稿日期:1900-01-01 出版日期:1997-04-10 发布日期:1997-04-10

Stud on Amplified Fragment Length Polymorphism at Locus D17S30 in Han Nationality of Yunnan Province

  • Received:1900-01-01 Online:1997-04-10 Published:1997-04-10

摘要: 应用PCR技术和小型聚丙烯酰胺凝胶电泳银染法, 对云南汉族人群D17S30位点扩增片段长度多态性进行了分析。在被检的105名无关个体中,共检出12个等位基因,41种基因型。等位基因频率范围在0.0048-0.2190之间,杂合度为83.81%,DP值为0.9647。观察的基因型分布符合Hardy-Weinber g定律。
Abstract:A study on amplified fragment length polymorphism(Amp-FLP)at locus D17S30 in Han nationality of Yunnan was carried out by using PCR followed by a high-resolution PAGE technique and silver staining.In a sample of 105 unrelated individuals,a total 12 different alleles and 41 genotypes were detected.The heterozygosity was 83.81% and the probability of discrimination(DP) was 0.9647.The distribution of observed genotypes obeyed the Hardy-Weinberg equilibrium.

关键词: PCR, Amp-FLP, 基因频率

Key words,
扩增片段长度多态性, D17S30位点, 回交, 耐盐性, 水稻, 耐盐突变系

Key words: NaCl-tolerance, Rice(Oryza sative), Mutant, Backcross