遗传 ›› 2003, Vol. 25 ›› Issue (1): 49-56.

• 论文 • 上一篇    下一篇

黄肉桃种质资源的RAPD分析

程中平1;2;陈志伟2;胡春根3;邓秀新3   

  1. 1.中国科学院武汉植物研究所,武汉 430074;2.武汉市林业果树研究所,武汉 430075;3.华中农业大学国家作物改良重点实验室,武汉 430070
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-02-10 发布日期:2003-02-10

Analysis of Yellow Peach Germplasm Revealed by RAPD Markers

CHENG Zhong-Ping1,2;CHEN Zhi-Wei2;HU Chun-Gen3;DENG Xiu-Xin3   

  1. 1.Wuhan Institute of Botany,CAS,Wuhan 430074,China;
    2.Wuhan Forestry and Fruit Research Institute,Wuhan 430075,China;
    3.State Key Laboratory for Genetic Improvement of Crops,Huazhong Agricultural University,Wuhan 430070,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-02-10 Published:2003-02-10

摘要: 利用RAPD技术,采用从200个十碱基随机物筛选的22个随机引物对37个黄肉桃品种的基因组DNA进行扩增,通过扩增的180个位点的谱带的聚类,分析供试黄肉桃的系统发育,运用特殊谱带,建立了黄肉桃的分子检索表,并提出了重点保存的黄肉桃种质。

关键词: 黄肉桃, RAPD, 系统发育, 分子检索表, 重点种质保存

Abstract: Genomic DNAs of 37 cultivars of yellow peach (Amgdalus persica) were amplified by RAPD (Randomly Amplified Polymorphic DNA) with 22 primers selected from 200 arbitrary 10 decamer primers.Phylogenetic relationship was analyzed through cluster of bands in 180 amplified sites.Molecular checking index and conservation of key germplasm were put forward according to special bands.