摘要：

根据多年生植物组织富含多酚、多糖的具体特性，对现有的DNA提取方法进行了改进。通过增加提取缓冲液中b-巯基乙醇用量，简化氯仿/异戊醇抽提液步骤，改用经-20℃预冷异丙醇沉淀DNA等，对CTAB法加以改进。改进后方法具有以下优点：（1）获得的DNA质量良好，提取过程无明显的DNA降解，基本上排除了多酚物质的干扰；（2）用获得的DNA进行Southern杂交，可得到理想的杂交信号，可满足相关的分子研究要求；（3）操作简便。Abstract It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharose, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.