遗传 ›› 2005, Vol. 27 ›› Issue (2): 181-184.

• 研究快报 • 上一篇    下一篇

逆转座子Tos17的激活与水稻白叶枯病成株抗性的关系

沙爱华;张端品   

  1. 华中农业大学作物遗传改良国家重点实验室国家农作物分子技术育种中心 武汉 430070
  • 收稿日期:2004-11-12 修回日期:2004-12-03 出版日期:2005-04-10 发布日期:2005-04-10
  • 通讯作者: 张端品

Relationship of Activation of Tos17 and Rice Adult Plant Resistance to Bacterial Blight

SHA Ai-Hua;ZHANG Duan-Pin   

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecluar Breeding, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2004-11-12 Revised:2004-12-03 Online:2005-04-10 Published:2005-04-10
  • Contact: ZHANG Duan-Pin

摘要: 为了研究水稻白叶枯病成株抗性是否与逆转子激活有关,运用SSAP (sequence-specific amplification polymorphism) 对成株抗性品种苗期和成株期接种白叶枯病原菌、清水接种及健康植株的基因组进行了逆转座子扫描。在筛选的约2000个逆转座子基因片段中,9个受苗期生长发育诱导激活,两个受成株期生长发育诱导激活,苗期和成株期各有3个受病原菌诱导激活。苗期生长发育诱导激活产生的逆转座子数目高于成株期,而病原菌诱导产生的逆转座子数目与成株期相当,表明水稻白叶枯病成株抗性可能与生长发育诱导的逆转座子激活相关。

关键词: 成株抗性, Tos17, 白叶枯病, 水稻

Abstract: to investigate whether rice adult plant resistance (APR) is related to activation of long terminal repeat (LTR) retroelements, polymorphism of LTR retroelemnts Tos17 are studied using SSAP (Sequence-Specific Amplification Polymorphism) in seedlings and adult plants of rice cultivar Wase Aikoku 3 inoculated with Xanthomonas oryzae pv. Oryzae, water and those untreated. Of 2000 fragments detected, 9 and 2 are activated in seedlings and adult plants induced by development, respectively; each 3 fragments are activated in seedlings and adult plants induced by pathogen, respectively. The number of activated Tos17 induced by development is more in seedlings than that in adult plants while it is equal due to pathogen induction in seedlings and adult plants, suggest that the activation of Tos17 induced by development probably associate with rice APR to bacterial blight.