遗传 ›› 2009, Vol. 31 ›› Issue (11): 1135-1140.

• 研究报告 • 上一篇    下一篇

水稻糙米重金属Cd含量的QTL分析

陈志德1, 2;王州飞1;贺建波1;仲维功2;王军2;杨杰2;张红生1

  

  1. 1. 南京农业大学作物遗传与种质创新国家重点实验室, 南京 210095;
    2. 江苏省农业科学院粮食作物研究所, 南京 210014

  • 收稿日期:2009-04-28 修回日期:2009-07-17 出版日期:2009-11-10 发布日期:2009-11-10
  • 通讯作者: 陈志德

Mapping of QTLs of Cd content in brown rice under Cd stress in rice

CHEN Zhi-De1, 2;WANG Zhou-Fei1;HE Jian-Bo1;ZHONG Wei-Gong2;WANG Jun2;YANG Jie2;ZHANG Hong-Sheng1   

  • Received:2009-04-28 Revised:2009-07-17 Online:2009-11-10 Published:2009-11-10

摘要:

镉(Cd2+)是一种分布较广泛、毒性较强的一种重金属, 文章利用韭菜青×IR26杂交衍生的一个重组自交系群体(Recombinant inbred lines, RIL)及构建的SSR分子遗传图谱, 对控制糙米中Cd2+含量的QTL进行分析, 为选育籽粒中Cd2+低吸收或低积累的水稻品种提供参考。结果表明, 在Cd2+胁迫(5 mg/kg)处理条件下, 共检测到2个与糙米Cd2+含量有关的QTLs, 分别位于水稻第11染色体上的标记RM6288-RM6544和RM167-RM5704之间, 其中qCCBR-11a对表型贡献率为11.17%, 加性效应0.089; qCCBR-11b对表型变异贡献率为7.66%, 加性效应0.075。相关分析显示, 糙米Cd2+含量与株高、每穗总粒数、每穗实粒数、结实率和千粒重等产量性状的相关性均不显著, 糙米中Cd2+含量是一个相对独立、由基因控制的遗传性状。

关键词: 水稻, Cd2+胁迫;糙米;QTL定位

Abstract:

A population of F8 recombinant inbred lines (RILs), derived from a cross between IR26 (Oryza sativa L. spp. indica) and Jiucaiqing (japonica), were used to identify the quantitative trait loci (QTLs) for Cd2+ content in brown rice under 5 mg/kg Cd2+ stress. Two QTLs, qCCBR-11a and qCCBR-11b, associated with the Cd2+ content in brown rice, were detected on chromosome 11. qCCBR-11a was located at the position between markers RM6288 and RM6544, accounting for 11.17% of the phenotypic variance with an additive effect value of 0.089. qCCBR-11b at the interval between markers RM167 and RM5704 explained 7.66% of the phenotypic variance with an additive effect value of 0.075. In addition, the correlation coefficients between Cd2+ content of brown rice and plant height, spikelets per panicle, filled grains per panicle, seed setting rate, and 1 000-grain weight were not significant. This suggested that the Cd2+ content in brown rice under Cd2+ stress was an independent genetic trait.