水稻生长发育多效基因DDF1的遗传分析与基因定位

doi:10.3724/SP.J.1005.2011.01374

遗传 ›› 2011, Vol. 33 ›› Issue (12): 1374-1379.doi: 10.3724/SP.J.1005.2011.01374

水稻生长发育多效基因DDF1的遗传分析与基因定位

李生平¹, 段远霖^1,2, 陈志伟^1,2, 官华忠^1,2, 王传蕾¹, 郑磊磊¹, 周元昌^1,2, 吴为人^1,2

1. 福建农林大学, 作物遗传育种与综合利用教育部重点实验室, 福州 350002 2. 福建农林大学, 福建省水稻分子标记辅助育种重点实验室, 福州 350002

收稿日期:2011-06-18 修回日期:2011-07-29 出版日期:2011-12-20 发布日期:2011-12-25
通讯作者: 吴为人 E-mail:wuwr@fjau.edu.cn
基金资助:
国家自然科学基金项目(编号：30970175, 30671122), 教育部博士点基金项目(编号：20093515110003), 国家高技术研究发展计划项目(863计划)(编号：2006AA10Z128), 福建省自然科学基金项目(编号：B0620001)资助

Genetic analysis and gene mapping of DDF1, a pleiotropic gene involving in both vegetable and reproductive growth in rice

Sheng-Ping¹, DUAN Yuan-Lin^1,2, CHEN Zhi-Wei^1,2, GUAN Hua-Zhong^1,2, WANG Chuan-Lei¹, ZHENG Lei-Lei¹, ZHOU Yuan-Chang^1,2, WU Wei-Ren^1,2

1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agricultural & Forestry University, Fuzhou 350002, China 2. Fujian Provincial Key Laboratory of Marker-Assisted Breeding of Rice, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received:2011-06-18 Revised:2011-07-29 Online:2011-12-20 Published:2011-12-25

摘要/Abstract

摘要： 植物中存在许多多效性基因, 它们在调控植物的营养生长与生殖发育过程中起着关键性作用。文章在籼稻育种材料中发现了一个植株显著矮化且花器官明显变异的突变体ddf1(dwarf and deformed flower 1)。遗传分析表明, 该突变体由单基因隐性突变所致, 这说明该基因是一个同时控制营养生长和生殖发育的多效性基因, 暂命名为DDF1。为了定位该基因, 将ddf1杂合体与热带粳稻品种DZ60杂交, 建立了F₂定位群体, 利用水稻RM系列微卫星标记, 通过混合分离分析(BSA)和小群体连锁分析, 将DDF1初步定位在水稻第6号染色体RM588和RM587标记之间, 与两标记的遗传距离分别为3.8 cM和2.4 cM。进一步利用已经公布的水稻基因组序列, 在初步定位的区间内开发新的SSR标记, 将DDF1定位在165 kb的区间内。该结果为克隆DDF1奠定了基础。

关键词: 水稻, 多效基因, DDF1, 遗传分析, 基因定位

Abstract: There are many pleiotropic genes playing key roles in regulating both vegetative growth and reproductive development in plants. A dwarf mutant of rice with deformed flowers, named as ddf1, was identified from indica rice breeding lines. Genetic analysis indicated that ddf1 was resulted from the recessive mutation of a single gene, temporarily named as DDF1. This result suggested that DDF1 is a pleiotropic gene, which controls both vegetative growth and reproductive development in rice. To map this gene, an F₂ population was developed by crossing the ddf1 heterozygote with the tropical japonica rice variety DZ60. By means of bulked segregant analysis and small population-based linkage analysis using the published RM-series rice SSR markers, DDF1 was preliminarily mapped in a region between markers RM588 and RM587 on chromosome 6 with the ge-netic distances of 3.8 cM and 2.4 cM to the two markers, respectively. By developing new SSR markers in this interval ac-cording to the published rice genome sequence, we further mapped DDF1 in a 165 kb interval. The results will fa-cilitate cloning of DDF1.

Key words: rice, pleiotropic gene, DDF1, genetic analysis, gene mapping

李生平，段远霖，陈志伟，官华忠，王传蕾，郑磊磊，周元昌，吴为人. 水稻生长发育多效基因DDF1的遗传分析与基因定位[J]. 遗传, 2011, 33(12): 1374-1379.

Sheng-Ping, DUAN Yuan-Lin, CHEN Zhi-Wei, GUAN Hua-Zhong, WANG Chuan-Lei, ZHENG Lei-Lei, ZHOU Yuan-Chang, WU Wei-Ren. Genetic analysis and gene mapping of DDF1, a pleiotropic gene involving in both vegetable and reproductive growth in rice[J]. HEREDITAS, 2011, 33(12): 1374-1379.

参考文献

[1] 罗琼, 王文明, 肖晗, 朱立煌, 周开达, 汪旭东, 王秀红. 一个新的水稻叶片和雌蕊发育异常突变体的遗传分析及其基因的分子标记定位. 科学通报, 2001, 46(15): 1277-1280.
[2] Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano HY. The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell, 2004, 16(2): 500-509.
[3] Smalle J, Kurepa J, Yang PZ, Emborg TJ, Babiychuk E, Kushnir S, Vierstra RD. The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling. Plant Cell, 2003, 15(4): 965-980.
[4] Wang H, Jones B, Li ZG, Frasse P, Delalande C, Regad F, Chaabouni S, Latché A, Pech JC, Bouzayen M. The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. Plant Cell, 2005, 17(10): 2676-2692.
[5] Radchuk R, Radchuk V, Weschke W, Borisjuk L, Weber H. Repressing the expression of the SUCROSE NONFERM ENTING-1-RELATED PROTEIN KINASE gene in pea embryo causes pleiotropic defects of maturation similar to an abscisic acid-insensitive phenotype. Plant Physiol, 2006, 140(1): 263-278.
[6] Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M, Kitano H, Matsuoka M. A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and or-ganless embryos. Plant J, 2009, 60(2): 227-241.
[7] Tian L, Chen ZJ. Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development. Proc Natl Acad Sci USA, 2001, 98(1): 200-205.
[8] Nelissen H, Clarke JH, Block MD, De Block S, Vander-haeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, van Li-jsebettens M. DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants. Plant Cell, 2003, 15(3): 639-654.
[9] Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL. Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis. Genetics, 2000, 156(3): 1363-1377.
[10] 秘彩莉, 刘旭, 张学勇. F-box蛋白质在植物生长发育中的功能. 遗传, 2006, 28(10): 1337-1342.
[11] González-Carranza GH, Rompa U, Peters JL, Bhatt AM, Wagstaff C, Stead AD, Roberts JA. HAWAIIAN SKIRT: An F-box gene that regulates organ fusion and growth in Arabidopsis. Plant Physiol, 2007, 144(3): 1370-1382.
[12] Deal RB, Kandasamy MK, McKinney EC, Meagher RB. The nuclear actin-related protein ARP6 is a pleiotropic developmental regulator required for the maintenance of FLOWERING LOCUS C expression and repres-sion of flowering in Arabidopsis. Plant Cell, 2005, 17(10): 2633-2646.
[13] Pei YX, Niu LF, Lu FL, Liu CY, Zhai JX, Kong XF, Cao XF. Mutations in the type II protein arginine methyltrans-ferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis. Plant Physiol, 2007, 144(4): 1913-1923.
[14] Yang J, Sardar H S, McGovern K R, Zhang YZ, Showalter AM. A lysine-rich arabinogalactan protein in Arabidopsis is essential for plant growth and development, including cell division and expansion. Plant J, 2007, 49(4): 629-640.
[15] Ge L, Chen H, Jiang JF, Zhao Y, Xu ML, Xu YY, Tan KH, Xu ZH, Chong K. Overexpression of OsRAA1 causes pleiotropic phenotypes in transgenic rice plants, including altered leaf, flower, and root development and root response to gravity. Plant Physiol, 2004, 135(3): 1502-1513.
[16] Xue WY, Xing YZ, Weng XY, Zhao Y, Tang WJ, Wang L, Zhou HJ, Yu SB, Xu CG, Li XH, Zhang QF. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40(6): 761-767.
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水稻生长发育多效基因DDF1的遗传分析与基因定位

Genetic analysis and gene mapping of DDF1, a pleiotropic gene involving in both vegetable and reproductive growth in rice

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