精子功能相关的蛋白质调控受精过程的研究进展

doi:10.3724/SP.J.1005.2014.0747

遗传 ›› 2014, Vol. 36 ›› Issue (8): 747-755.doi: 10.3724/SP.J.1005.2014.0747

精子功能相关的蛋白质调控受精过程的研究进展

陈志林¹, 冯美莹¹, 陈预明¹, 卫恒习¹, 李莉¹, 吴同山², 张守全¹

1. 华南农业大学动物科学学院,国家生猪种业工程技术研究中心,广州 510642;
2. 东莞市畜牧科学研究所,东莞 523086

收稿日期:2014-03-22 出版日期:2014-08-20 发布日期:2013-07-19
通讯作者: 张守全,教授,博士生导师,研究方向：动物遗传与繁育。E-mail:sqzhang@scau.edu.cn
作者简介:陈志林,在读硕士研究生,专业方向：动物遗传与繁育。Tel:020-85285897;E-mail:qq505878698@sina.com
基金资助:
国家科技支撑计划项目(编号：2011BAD19B03),国家重点基础研究发展计划(973计划)项目(编号：2011CB944202; 2009CB941601; 2010CB945001)和东莞市科技计划项目(编号：2012108102049)资助

The progress of sperm functional proteins regulating the process of fertilization

Zhilin Chen¹, Meiying Feng¹, Yuming Chen¹, Hengxi Wei¹, Li Li¹, Tongshan Wu², Shouquan Zhang¹

1. National Engineering Research Center For Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. Dongguan Institute of Animal Science, Dongguan 523086, China

Received:2014-03-22 Online:2014-08-20 Published:2013-07-19

摘要/Abstract

摘要： 哺乳动物的受精过程涉及到精子一系列的功能活动,如精子在雌性生殖道的运行、精子的超活化与获能、顶体反应以及精卵融合等。在精子经历的这一系列过程中,精子功能相关的蛋白质发挥着不可或缺的作用,这些蛋白分子的正常与否与雄性个体的繁殖力高低密切相关,因此精子功能相关的蛋白质能够作为评定哺乳动物精液受精能力的生物标记。文章主要对哺乳动物精子功能相关的蛋白质进行了综述,以阐述相关蛋白分子对精子运动活力、精子获能、顶体反应、透明带穿入和精卵融合等方面的重要作用以及这些蛋白分子在家畜遗传改良上的潜在应用。

关键词: 受精, 精子, 蛋白质, 生物标记, 繁殖力

Abstract: The process of mammalian fertilization involves a series of sperm functional activities, including the sperm transportation, hyperactivation and capacitation, acrosome reaction and sperm-egg fusion, etc. The semen proteins play indispensable roles in these processes, and they are closely associated with the fecundity of males. So these proteins can be biomarkers to evaluate the fertilization capacity of mammalian semen. In this review, we mainly introduce some semen proteins relevant to spermatozoa functions and illustrate their important regulatory roles on the fertilization processes, involving spermatozoa motility, capacitation, acrosome reaction, penetrating the zona pellucida and sperm-egg fusion, etc. Moreover, their potential applications in the evaluation of heredity in livestock are also summarized.

Key words: fertilization, spermatozoa, protein, biomarkers, fecundity

陈志林, 冯美莹, 陈预明, 卫恒习, 李莉, 吴同山, 张守全. 精子功能相关的蛋白质调控受精过程的研究进展[J]. 遗传, 2014, 36(8): 747-755.

Zhilin Chen, Meiying Feng, Yuming Chen, Hengxi Wei, Li Li, Tongshan Wu, Shouquan Zhang. The progress of sperm functional proteins regulating the process of fertilization[J]. HEREDITAS(Beijing), 2014, 36(8): 747-755.

参考文献

[1] A, Karsani SA, Nathan S. Mammalian sperm fertility related proteins . Int J Med Sci , 2013, 10(12): 1649-1657.
[2] J, Jemel I, Tanemoto A, Taketomi Y, Payre C, Coatrieux C, Sato H, Yamamoto K, Masuda S, Pernet- Gallay K, Pierre V, Hara S, Murakami M, De Waard M, Lambeau G, Arnoult C. Group X phospholipase A2 is rel-eased during sperm acrosome reaction and controls fertility outcome in mice . J Clin Invest , 2010, 120(5): 1415-1428.
[3] SK, Bhandari B. Acrosome reaction: relevance of zona pellucida glycoproteins . Asian J Androl , 2011, 13(1): 97-105.
[4] P, Myles DG. Cell-cell membrane fusion during mammalian fertilization . FEBS Lett , 2007, 581(11): 2174- 2180.
[5] GG, Woolley DM. Basal sliding and the mechanics of oscillation in a mammalian sperm flagellum . Biophys J , 2004, 87(6): 3934-3944.
[6] J, Diaz ES, Morales P. Kinases, phosphatases and proteases during sperm capacitation . Cell Tissue Res , 2012, 349(3): 765-782.
[7] PE. Understanding the molecular basis of sperm capacitation through kinase design . Proc Natl Acad Sci USA , 2009, 106(3): 667-668.
[8] IA, Espinosa F, Edwards J, Sosnik J, De La Vega- Beltrán JL, Hockensmith JW, Kopf GS, Darszon A, Visconti PE. Involvement of a Na + /HCO -3 cotransporter in mouse sperm capacitation . J Biol Chem , 2003, 278(9): 7001-7009.
[9] D, Finkelstein M, Breitbart H. Mechanism of sperm capacitation and the acrosome reaction: role of pro-tein kinases . Asian J Androl , 2012, 14(6): 816-821.
[10] PV, Botchkina IL, Fedorenko A, Kirichok Y. Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel . Cell , 2010, 140(3): 327-337.
[11] B, Suarez SS. Bovine sperm hyperactivation is promoted by alkaline-stimulated Ca 2+ influx . Biol Reprod , 2007, 76(4): 660-665.
[12] CM, Santos T, Hernández-Cruz A, Darszon A. Properties of a novel pH-dependent Ca 2+ permeation path-way present in male germ cells with possible roles in sper-matogenesis and mature sperm function . J Gen Physiol , 1998, 112(1): 33-53.
[13] Y, Navarro B, Clapham DE. Whole-cell patch- clamp measurements of spermatozoa reveal an alkaline- activated Ca 2+ channel . Nature , 2006, 439(7077): 737-740.
[14] B, Kirichok Y, Clapham DE. KSper, a pH-sen-sitive K+ current that controls sperm membrane potential . Proc Natl Acad Sci USA , 2007, 104(18): 7688-7692.
[15] V, Jouannet P, Pignot-Paintrand I, Feneux D. Effects of pH on the reactivation of human spermatozoa demembranated with Triton X-100 . Mol Reprod Dev , 1991, 29(2): 157-162.
[16] HC, Granish KA, Suarez SS. Hyperactivated motility of bull sperm is triggered at the axoneme by Ca 2+ and not cAMP . Dev Biol , 2002, 250(1): 208-217.
[17] M, Megnagi B, Ickowicz D, Breitbart H. Regulation of sperm motility by PIP2(4, 5) and actin polymerization . Dev Biol , 2013, 381(1): 62-72.
[18] D, Wegner A. Gelsolin as a calcium-regulated actin filament-capping protein . Eur J Biochem , 2000, 267(14): 4339-4345.
[19] PA, Iida K, Yin HL, Stossel TP. Polyphosphoinositide micelles and polyphosphoinositide-containing vesicles dissociate endogenous gelsolin-actin complexes and promote actin assembly from the fast-growing end of actin filaments blocked by gelsolin . J Biol Chem , 1987, 262(25): 12228-12236.
[20] FM, Wijnand E, van de Lest CHA, Colenbrander B, van Golde LMG, Gadella BM. Capacitation dependent activation of tyrosine phosphorylation generates two sperm head plasma membrane proteins with high primary binding affinity for the zona pellucida . Mol Reprod Dev , 2001, 60(1): 107-115.
[21] PE, Moore GD, Bailey JL, Leclerc P, Connors SA, Pan D, Olds-Clarke P, Kopf GS. Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway . Deve l opment , 1995, 121(4): 1139-1150.
[22] JS, Gardner TK, Kanous KS, Lindemann CB. The interaction of pH and cyclic adenosine 3', 5'-monopho-s

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

精子功能相关的蛋白质调控受精过程的研究进展

The progress of sperm functional proteins regulating the process of fertilization

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	田璐妍, 黄小珍. 植物开花调控中蛋白质相分离机制在从头驯化中的应用价值[J]. 遗传, 2023, 45(9): 754-764.
[2]	吴丹丹, 朱明昆, 方忠艳, 马伟. 植物B染色体的分子结构组成及遗传机制研究进展[J]. 遗传, 2022, 44(9): 772-782.
[3]	朱艳, 魏明, 周晓, 邓林华, 仇剑, 李果, 周世强, 谢浩, 李德生, 王承东. 大熊猫(Ailuropoda melanoleuca) miRNA研究进展[J]. 遗传, 2021, 43(9): 849-857.
[4]	蒋婧, 赵安麒, 谢甜, 陈淑藯, 李劲松. 全基因组蛋白质的标签细胞和小鼠资源库建设[J]. 遗传, 2021, 43(7): 704-714.
[5]	张星雨, 祝天喻, 张清荣, 郭雪江, 王铖, 靳光付, 胡志斌. 精子发生障碍的遗传学研究进展[J]. 遗传, 2021, 43(5): 473-486.
[6]	袁露, 葛婷婷, 牛长敏, 徐文华, 郑英. 精子变形过程中组蛋白-鱼精蛋白替换调控机制[J]. 遗传, 2021, 43(12): 1121-1131.
[7]	葛婷婷, 袁露, 徐文华, 郑英. 哺乳动物纤毛/鞭毛内运输在精子形成中的作用及机制研究进展[J]. 遗传, 2021, 43(11): 1038-1049.
[8]	肇涛澜, 张硕, 钱文峰. 翻译延伸的顺式调控机理与生物学效应[J]. 遗传, 2020, 42(7): 613-631.
[9]	钟亚楠, 牛长敏, 夏蒙蒙, 郑英. 精子尾部发育相关蛋白研究进展[J]. 遗传, 2020, 42(6): 524-535.
[10]	于雅洁, 邱峰, 张新安. TSR1突变导致先天性白内障及其在晶状体中的表达[J]. 遗传, 2020, 42(2): 161-171.
[11]	吕丹丹,张媛雅,葛海涛,黄夏禾,汪迎春. 大规模膜蛋白质组鉴定技术进展[J]. 遗传, 2019, 41(9): 863-874.
[12]	赵鑫,杨化强. 大动物精原干细胞研究进展[J]. 遗传, 2019, 41(8): 686-702.
[13]	肖娟, 王讯, 罗毅, 李晓开, 李学伟. 附睾小体功能蛋白及sRNA研究进展[J]. 遗传, 2018, 40(3): 197-206.
[14]	袁志恒,赵艳梅. piRNA/PIWI功能调控与精子发生[J]. 遗传, 2017, 39(8): 683-691.
[15]	胡立桥,周兆才,田伟. Hippo信号通路结构生物学研究进展[J]. 遗传, 2017, 39(7): 659-674.