遗传 ›› 2007, Vol. 29 ›› Issue (9): 1110-1120.doi: 10.1360/yc-007-1110

• 研究报告 • 上一篇    下一篇

水稻籼粳交F2、F6群体遗传连锁图谱的比较分析

梁永书1, 2, 3, 彭勇1, 3, 叶少平1, 3, 李平1, 3, 孙林静2, 马忠友2, 李艳萍2   

  1. 1. 四川农业大学水稻研究所, 温江 611130; 2. 天津农业科学院作物研究所, 天津300112 3. 西南作物基因资源与遗传改良教育部重点实验室, 雅安 625014;

  • 收稿日期:2007-03-09 修回日期:2007-05-07 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 梁永书

Comparison of genetic linkage maps based on F2、F6 populations derived from rice subspecies cross

LIANG Yong-Shu1, 2, 3, PENG Yong 1, 3, YE Shao-Ping 1, 3, LI Ping1, 3, SUN Lin-Jing2, MA Zhong-You2, LI Yan-Ping2
  

  1. 1. Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China; 2. Institute of Crops, Academy of Agricultural Sciences, Tianjin 300112;
    3. China Key Laboratory of Southwest Crop Genetic Resources and Improvement Ministry of Education, Ya′an 625014, China
  • Received:2007-03-09 Revised:2007-05-07 Online:2007-09-10 Published:2007-09-10
  • Contact: LIANG Yong-Shu

摘要:

以部分基因组和全基因组测序水稻籼稻(O sativa L. indica)品种“培矮64S”(Pei’ai 64S♀)和粳稻(O sativa L. japonica)品种“日本晴”(Nipponbare♂)为构图亲本, 选取F2代180个株系为作图群体, 构建含138个微卫星位点的水稻遗传连锁图谱, 覆盖基因组2 046.2 cM, 平均图距17.1 cM, 即F2 图谱; 采用单粒传法获得F2:6 代330个株系, 用相同的多态性标记分析F6群体, 构建含92个标记连锁图谱, 覆盖基因组2 563.5 cM, 平均图距27.86 cM, 即F6图谱; F2、F6图谱在连锁群数、定位标记数、标记的位置顺序、遗传图距、平均图距等方面发生了较大变化, 并对产生这些差异的原因进行了初步分析。

关键词: 比较分析, 偏分离, F2、F6遗传图谱, 微卫星标记, 水稻

Abstract:

A F2 population containing 180 lines, which was derived from the cross between the partially sequenced indica variety “Pei’ai 64S” and the completely sequenced japonica variety “Nipponbare” , was used to construct a genetic linkage framework map (referred to as F2 map), which included 138 microsatellite sites and covered 1737.81 cM of total genomic length, an average distance of 11.90 cM. Single seed descent F2:6 population with 330 lines was used to construct a genetic linkage map (known as F6 map) using 92 markers. The total genomic length and average distance were 2563.5 cM and 27.86 cM, respectively. The F2 and F6 maps differed in linkage groups, mapped markers, sequenced order of markers, ge-netic distance and average distance on the maps. Preliminary analysis about these difference was carried out.