水稻Dwarf1移码突变的新突变体鉴定

doi:10.3724/SP.J.1005.2011.00397

遗传 ›› 2011, Vol. 33 ›› Issue (4): 397-403.doi: 10.3724/SP.J.1005.2011.00397

水稻Dwarf1移码突变的新突变体鉴定

陈华夏, 周成博, 邢永忠

华中农业大学作物遗传改良国家重点实验室, 国家植物基因中心(武汉), 武汉 430070

收稿日期:2010-09-30 修回日期:2010-12-10 出版日期:2011-04-20 发布日期:2011-04-25
通讯作者: 邢永忠 E-mail:yzxing@mail.hzau.edu.cn
基金资助:
国家自然科学基金项目(编号：30971749)和国家973项目(编号：2010CB125901)及转基因生物新品种培育重大专项(编号：2008ZX08009-001)资助

A new rice dwarf1 mutant caused by a frame-shift mutation

CHEN Hua-Xia, ZHOU Cheng-Bo, XING Yong-Zhong

National Key Laboratory of Crop Genetic Improvement, National Plant Gene Research Center (Wuhan), Huazhong Agricultural Uni-versity, Wuhan 430070, China

Received:2010-09-30 Revised:2010-12-10 Online:2011-04-20 Published:2011-04-25
Contact: XING Yong-Zhong E-mail:yzxing@mail.hzau.edu.cn

摘要/Abstract

摘要： 从一批水稻品种“中花11” 组织培养苗里分离到一个矮化突变株“C6PS”, 它的T2代群体株高呈现3:1分离。利用该群体矮化单株与“珍汕97”、“牡丹江8”构建2个F₂群体F₂(CZ)、F₂(CM), 两个群体中高株与矮株均呈现3:1分离, 证明该性状变异为单基因控制。“C6PS” 表现型与已经报道的Dwarf1隐性突变体“d1”相似, 以D1附近标记RM430检测F₂(CZ) 群体基因型, 结果显示群体表型与RM430基因型呈极显著相关(P=0.0001), 将该基因初步定位于Dwarf1附近。对“C6PS”及“中花11”进行D1序列分析显示, 突变株中D1基因在其第九个外显子与第九个内含子的剪接位点上发生6个碱基的缺失, 根据缺失两侧序列设计C6PS-D1L/R标记, 在T₂代群体该标记与表型呈现共分离, 表明“C6PS”是一种新的Dwarf1突变体。cDNA测序显示突变体d1基因转录产物发生26个碱基的缺失, 导致移码产生终止突变, 从而无法翻译出有功能的Gα蛋白, 因此, 它是一个Gα功能缺失突变体。叶倾斜度检测显示“C6PS”对油菜素内酯响应比野生型“中花11”弱。

关键词: 水稻, 油菜素内酯, 终止突变, 矮化突变株, Gα蛋白

Abstract: A dwarf mutant C6PS, which has the similar phenotype as the recessive mutant Dwarf1 (d1), was produced from tissue-cultured plants of Zhonghua 11. In its progeny (T2), the ratio of tall to dwarf plants was in agreement with the expected segregation ratio (3:1) of a single Mendelian inheritance gene, which indicated that the variation of plant height is caused by a single gene. To locate the mutation, C6PS was crossed with Zhenshan 97 and Mudanjiang 8 for producing two F₂ populations of F₂ (CM) and F₂ (CZ), respectively. The plant height in each F₂ population also showed the same segregation pattern as that in T2 generation. SSR marker RM430 closely linked to Dwarf1 was preferentially used to genotype the F₂ (CZ) population because C6PS showed the similar phenotype to d1 mutant. RM430 was significantly associated with plant height, which indicated that the mutant gene might be D1. Comparative sequencing of D1 between C6PS and Zhonghua 11 showed a 6 bp deletion occurred in the splice site of its ninth exon. The marker C6PS-D1L/R designed on the 6 bp deletion was co-segregated with plant height in T2 generation. The results indicated that C6PS was a new mutant of D1. This mutation led to a 26 bp deletion of the transcript and resulted in a frame-shift mutation and a premature stop codon in C6PS, which could not translate the functional Gα protein. C6PS was weakly sensitive to Brassinolide based on the leaf inclination angle test.

Key words: rice, protein, premature stop, Brassinolide, dwarf mutant, G&alpha

陈华夏，周成博，邢永忠. 水稻Dwarf1移码突变的新突变体鉴定[J]. 遗传, 2011, 33(4): 397-403.

CHEN Hua-Jia, ZHOU Cheng-Bo, GENG Yong-Zhong. A new rice dwarf1 mutant caused by a frame-shift mutation[J]. HEREDITAS, 2011, 33(4): 397-403.

参考文献

[1] Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishi-mura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush GS, Kitano H, Matsuoka M. Green revolution: A mutant gibberellin-synthesis gene in rice. Nature, 2002, 416(6882): 701-702.
[2] Ashikari M, Wu JZ, Yano M, Sasaki T, Yoshimura A. Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein. Proc Natl Acad Sci USA, 1999, 96(18): 10284-10289.
[3] Fujisawa Y, Kato T, Ohki S, Ishikawa A, Kitano H, Sasaki T, Asahi T, Iwasaki Y. Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice. Proc Natl Acad Sci USA, 1999, 96(13): 7575-7580.
[4] Oki K, Inaba N, Kitano H, Takahashi S, Fujisawa Y, Kato H, Iwasaki Y. Study of novel d1 alleles, defective mutants of the α subunit of the heterotrimeric G-protein in rice. Genes Genet Syst, 2009, 84(1): 35-42.
[5] Perfus-Barbeoch L, Jones AM, Assmann SM. Plant heterotrimeric G protein function: insights from Arabidopsis and rice mutants. Curr Opin Plant Biol, 2004, 7(6): 719-731.
[6] Assmann SM. Plant G Proteins, Phytohormones, and plas-ticity: Three questions and a speculation. Sci STKE, 2004, 264: re20.
[7] Ishikawa A, Tsubouchi H, Iwasaki Y, Asahi T. Molecular cloning and characterization of a cDNA for the α subunit of a G protein from rice. Plant Cell Physiol, 1995, 36(2): 353-359.
[8] Ishikawa A, Iwasaki Y, Asahi T. Molecular cloning and characterization of a cDNA for the rβ subunit of a G pro-tein from rice. Plant Cell Physiol, 1996, 37(2): 223-228.
[9] Kato C, Mizutani T, Tamaki H, Kumagai H, Kamiya T, Hirobe A, Fujisawa Y, Kato H, Iwasaki Y. Characterization of heterotrimeric G protein complexes in rice plasma membrane. Plant J, 2004, 38(2): 320-331.
[10] Ueguchi-Tanaka M, Fujisawa Y, Kobayashi M, Ashikari M, Iwasaki Y, Kitano H, Matsuoka M. Rice dwarf mutant d1, which is defective in the α subunit of the het-erotrimeric G protein, affects gibberellin signal transduction. Proc Natl Acad Sci USA, 2000, 97(21): 11638-11643.
[11] Suharsono U, Fujisawa Y, Kawasaki T, Iwasaki Y, Satoh H, Shimamoto K. The heterotrimeric G protein α subunit acts upstream of the small GTPase Rac in disease resistance of rice. Proc Natl Acad Sci USA, 2002, 99(20): 13307-13312.
[12] Lieberherr D, Thao NP, Nakashima A, Umemura K, Kawasaki T, Shimamoto K. A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice. Plant Physiol, 2005, 138(3): 1644-1652.
[13] Assmann SM. G Protein regulation of disease resistance during infection of rice with rice blast fungus. Sci STKE, 2005, 310: cm13.
[14] Iwasaki Y, Fujisawa Y, Kato H. Function of heterotrimeric G protein in gibberellin signaling. Plant Growth Regul, 2003, 22(2): 126-133.
[15] Oki K, Inaba N, Kitagawa K, Fujioka S, Kitano H, Fuji-sawa Y, Kato H, Iwasaki Y. Function of the α subunit of rice heterotrimeric G protein in Brassinosteroid signaling. Plant Cell Physiol, 2009, 50(1): 161-172.
[16] Wada K, Marumo S, Ikekawa N, Morisaki M, Mori K. Brassinolide and Homobrassinolide promotion of lamina inclination of rice seedling. Plant Cell Physiol, 1981, 22(2): 323-325.
[17] Murray MG, Thompson WF. Rapid isolation of high mo-lecular weight plant DNA. Nucleic Acid Res, 1980, 8(19):4321-4326.
[18] 张玉山, 白旭峰. 一种简单快速高分辨率的PAGE胶显带方法. 遗传, 2008, 30(2): 251-254.
[19] Jones AM, Assmann SM. Plants: the latest model system for G-protein research. EMBO Rep, 2004, 5(6): 572-578.
[20] Assmann SM. G Proteins go green: A plant G protein signaling FAQ sheet. Science, 2005, 310(5745): 71-73.
[21] Wang L, Xu YY, Ma QB, Li D, Xu ZH, Chong K. Hetero-

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

水稻Dwarf1移码突变的新突变体鉴定

A new rice dwarf1 mutant caused by a frame-shift mutation

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	卞中, 曹东平, 庄文姝, 张舒玮, 刘巧泉, 张林. 水稻分子设计育种启示：传统与现代相结合[J]. 遗传, 2023, 45(9): 718-740.
[2]	刘向东, 吴锦文, 陆紫君, Muhammad Qasim Shahid. 同源四倍体水稻：低育性机理、改良与育种展望[J]. 遗传, 2023, 45(9): 781-792.
[3]	郝小花, 胡爽, 赵丹, 田连福, 谢子靖, 吴莎, 胡文俐, 雷晗, 李东屏. OsGA3ox通过合成不同活性GA调控水稻育性及株高[J]. 遗传, 2023, 45(9): 845-855.
[4]	郑镇武, 赵宏源, 梁晓娅, 王一珺, 王驰航, 巩高洋, 黄金燕, 张桂权, 王少奎, 刘祖培. 水稻qGL3.4调控籽粒大小与株型[J]. 遗传, 2023, 45(9): 835-844.
[5]	陈明江, 刘贵富, 肖叶青, 余泓, 李家洋. 中科发早粳1号分子设计育种[J]. 遗传, 2023, 45(9): 829-834.
[6]	刘永强, 李威威, 刘昕禹, 储成才. 水稻分蘖氮响应调控机理研究进展[J]. 遗传, 2023, 45(5): 367-378.
[7]	林建辉, 刘自广, 张楠, 严容, 张楠, 何鑫淼, 王文涛, 刘娣, 吴娟. 拟南芥ABI5基因对BR胁迫响应及其对下胚轴生长的调节作用[J]. 遗传, 2021, 43(9): 901-909.
[8]	李姗, 黄允智, 刘学英, 傅向东. 作物氮肥利用效率遗传改良研究进展[J]. 遗传, 2021, 43(7): 629-641.
[9]	张昌泉, 冯琳皓, 顾铭洪, 刘巧泉. 江苏省水稻品质性状遗传和重要基因克隆研究进展[J]. 遗传, 2021, 43(5): 425-441.
[10]	代航, 李延, 刘树春, 林磊, 吴娟燕, 张志杰, 彭崎春, 李楠, 张向前. 类伸展蛋白OsPEX1对水稻花粉育性的影响[J]. 遗传, 2021, 43(3): 271-279.
[11]	闫凌月, 张豪健, 郑雨晴, 丛韫起, 刘次桃, 樊帆, 郑铖, 袁贵龙, 潘根, 袁定阳, 段美娟. 转录因子OsMADS25提高水稻对低温的耐受性[J]. 遗传, 2021, 43(11): 1078-1087.
[12]	胡雅丽, 戴睿, 刘永鑫, 张婧赢, 胡斌, 储成才, 袁怀波, 白洋. 水稻典型品种日本晴和IR24根系微生物组的解析[J]. 遗传, 2020, 42(5): 506-518.
[13]	张桂权. 基于SSSL文库的水稻设计育种平台[J]. 遗传, 2019, 41(8): 754-760.
[14]	于好强,孙福艾,冯文奇,路风中,李晚忱,付凤玲. 转录因子BES1/BZR1调控植物生长发育及抗逆性[J]. 遗传, 2019, 41(3): 206-214.
[15]	刘次桃, 王威, 毛毕刚, 储成才. 水稻耐低温逆境研究：分子生理机制及育种展望[J]. 遗传, 2018, 40(3): 171-185.