遗传 ›› 2013, Vol. 35 ›› Issue (11): 1253-1264.doi: 10.3724/SP.J.1005.2013.01253

• 综述 • 上一篇    下一篇

非编码DNA序列的功能及其鉴定

秦丹1,2, 徐存拴1,2   

  1. 1. 河南师范大学生命科学学院, 新乡 453007; 
    2. 河南省-科技部共建细胞分化调控国家重点实验室培育基地, 新乡 453007
  • 收稿日期:2013-06-07 修回日期:2013-07-31 出版日期:2013-11-20 发布日期:2013-10-23
  • 通讯作者: 徐存拴, 博士, 教授, 研究方向:细胞分化调控。 E-mail:xucs@x263.net
  • 作者简介:秦丹, 硕士研究生, 专业方向:细胞分化调控。Tel: 18336063263; E-mail: qindan2008@126.com
  • 基金资助:

    国家重点基础研究发展计划项目(973计划)(编号:2012CB722304)和河南省基础与前沿技术研究计划项目(编号:102300413213)资助

Characterization and identification of functional elements in non-coding DNA sequences

  1. 1. College of Life Science, Henan Normal University, Xinxiang 453007, China; 
    2. State Key Laboratory Breeding Base Co-sponsored by Henan & Ministry of Science and Technology for Cell Differentiation Regulation, Xinxiang 453007, China
  • Received:2013-06-07 Revised:2013-07-31 Online:2013-11-20 Published:2013-10-23

摘要:

非编码DNA序列是指基因组中不编码蛋白质的DNA序列。这些序列可以结合调节因子、转录为功能性RNA、单独或协同地调节生理活动和病理过程。文章围绕基因表达调控作用, 总结了近几年非编码DNA序列的研究成果, 对其结构、功能和可能的作用机制进行了初步阐述, 介绍了目前鉴定非编码DNA序列中功能元件的计算方法和实验技术, 并对非编码DNA未来的研究进行了展望。

关键词: 非编码DNA序列, 基因表达调控, 功能元件, 转录因子结合位点, 非编码RNA, 表观遗传学

Abstract:

The non-coding DNA sequences refer to non-protein-coding DNA sequences in genome. These sequences can bind with transcription factors or be transcribed as functional RNAs, thus participating in the regulation of many physiological activities and pathological processes. Aiming at gene expression regulation, this review focuses on the recent progress of non-coding DNA and illustrates their structures, functions and potential acting mechanisms. Meanwhile, some computational and experimental methods of identifying functional elements in the non-coding DNAs are introduced. Finally, further studies in this field are proposed.

Key words: non-coding DNA sequences, gene expression regulation, functional elements, transcription factor bind-ing sites, non-coding RNAs, epigenetics