遗传 ›› 2022, Vol. 44 ›› Issue (6): 478-490.doi: 10.16288/j.yczz.22-007

• 综述 • 上一篇    下一篇

功能性微肽通量发现和功能验证的研究进展

吕雪(), 李帮洁, 徐寒梅()   

  1. 中国药科大学,江苏省合成多肽药物发现与评价工程中心,南京 211198
  • 收稿日期:2022-02-28 修回日期:2022-04-02 出版日期:2022-06-20 发布日期:2022-04-14
  • 通讯作者: 徐寒梅 E-mail:13837189145@163.com;xuhanmei6688@126.com
  • 作者简介:吕雪,在读硕士研究生,专业方向:微生物与生化药学。E-mail: 13837189145@163.com
  • 基金资助:
    国家科技重大专项编号(2019ZX09301-124);中国药科大学天然药物活性组分与药效国家重点实验编号(SKLNMZZ202028);中国博士后科学基金资助项目编号(2020M681787);江苏省自然科学基金资助编号(BK20210418)

Research progress on high throughput discovery and functional verification of functional micropeptides

Lv Xue(), Li Bangjie, Xu Hanmei()   

  1. Engineering Research Center of Synthetic Peptide Drug Discovery and Evaluation of Jiangsu Province, China Pharmaceutical University, Nanjing 211198, China
  • Received:2022-02-28 Revised:2022-04-02 Online:2022-06-20 Published:2022-04-14
  • Contact: Xu Hanmei E-mail:13837189145@163.com;xuhanmei6688@126.com
  • Supported by:
    Supported by the National Science and Technology Major Projects No(2019ZX09301-124);the Project Program of State Key Laboratory of Natural Medicines No(SKLNMZZ202028);China Postdoctoral Science Foundation No(2020M681787);the Natural Science Foundation of Jiangsu Province No(BK20210418)

摘要:

随着计算生物学和深度测序技术的飞速发展,愈来愈多的研究表明大量之前未被注释、隐藏在非编码RNA中的非经典开放阅读框(open reading frame, ORF)具有编码功能性微肽(micropeptide)的能力。本文对微肽的基因来源、生物性质、预测方法和功能验证的研究现状和技术策略展开综述,以期为后续开展微肽发现、研究调控机制以及新靶点、生物标志物的开发等提供理论基础和参考依据。

关键词: 非编码RNA, sORFs, 功能性微肽

Abstract:

With the rapid development of computational biology and deep sequencing technology, more and more studies have shown that a large number of non-classical open reading frames that have not been annotated and hidden in non-coding RNA can encode functional micropeptide. This article reviewed the current research status and technology strategy of gene sources, biological properties, predicted methods and functional verification of micropeptide, providing theoretical and reference basis for the subsequent discovery of micropeptides, research on regulatory mechanisms and development of novel targets and biomarkers.

Key words: noncoding RNA, sORFs, functional micropeptides