遗传 ›› 1998, Vol. 20 ›› Issue (1): 20-23.

• 论文 • 上一篇    下一篇

云南绵羊线粒体DNA遗传多态性研究

兰蓉1; 洪琼花1; 高源汉1; 张俊1; 宿兵2; 王文2; 刘爱华2; 张亚平2; 施立明2 LAN Rong1;HONG Qiong-Hua1;GAO Yuan-Han1;ZHANG Jun1;SU Bing2;WANG Wen2;ZHANGYa-Ping2;SHI Li-Ming2   

  1. 1.云南省畜牧兽医研究所, 昆明 650224 2.中国科学院昆明动物研究所细胞与进化开放研究实验室,昆明 650223 1.Yunnan Institute of Husbandry and Veterinary,Kunming 650224 2.Laboratory of Cellular and Molecular Evolution,Kunming Institute of Zoolohgy,Chinese Academy of Sciences,Kunming 650223
  • 收稿日期:1900-01-01 出版日期:1998-02-10 发布日期:1998-02-10

Mitochondrial DNA Polymorphism in Sheep from Yunnan

  • Received:1900-01-01 Online:1998-02-10 Published:1998-02-10

摘要: 本研究用12种识别六碱基的限制性内切酶对来自云南省3个地方绵羊品种11个样本的线粒体DNA(mtDNA)进行了限制性片段长度多态性分析(RFLP),结果表明,云南绵羊群体平均核苷歧异度为0.086%,平均遗传距离为0.00 4,说明mtDNA变异度很低,遗传多样性贫乏,提示云南绵羊可能起源于同一个共同祖先;昭通绵羊与德钦绵羊距离较远,大约分歧于30万年前,德钦绵羊与腾冲绵羊大约分歧于11万年前,而腾冲绵羊与昭通绵羊也于18万年前发生分歧。
Abstract:mtDNA from 11 sheeps samples of Deqin,Zhaotong and Tengchong in Yunnan province was analyzed with 12 restriction endonucleases.14 restriction types were observed which could sorted into 3 haplotypes.The estimated number of nucleotide substitutions per site(π)in Yunnan sheep was 0.086% and the mean genetic distance(P) was 0.004.This results might show a pattern of single origin of Yunnan sheep.Divergencetime of the native sheep flocks from three districts of Yunnan were estimated on the mean rate of sequence divergence of 0.01 per million years in mtDNA.

关键词: Yunnan sheep, 遗传多样性
Key words,
RFLP, 线粒体DNA, 云南绵羊, 数量性状座位(QTL), RAPD, 重叠基因, 水稻

Key words: RAPD QTLs Rice Overlapping