iPSCs遗传稳定性与重编程机制的研究进展

doi:10.3724/SP.J.1005.2014.0879

遗传 ›› 2014, Vol. 36 ›› Issue (9): 879-887.doi: 10.3724/SP.J.1005.2014.0879

iPSCs遗传稳定性与重编程机制的研究进展

任才芳，孙红艳，王立中，张国敏，樊懿萱，颜光耀，王丹，王锋

南京农业大学，江苏省家畜胚胎工程实验室，南京 210095

收稿日期:2014-04-17 出版日期:2014-09-20 发布日期:2014-09-20
通讯作者: 王锋，博士，教授，研究方向：动物胚胎工程。E-mail: caeet@njau.edu.cn E-mail:2012105033@njau.edu.cn
作者简介:任才芳，博士研究生，研究方向：胚胎干细胞。
基金资助:
国家自然科学基金项目(编号：31272443)资助

Reprogramming mechanism and genetic stability of induced pluripotent stem cells (iPSCs)

Caifang Ren, Hongyan Sun, Lizhong Wang, Guomin Zhang, Yixuan Fan, Guangyao Yan, Dan Wang, Feng Wang

Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China

Received:2014-04-17 Online:2014-09-20 Published:2014-09-20

摘要/Abstract

摘要： 诱导性多能干细胞(Induced pluripotent stem cells, iPSCs)是采用特定转录因子，将体细胞重编程为具有多能性的干细胞。iPSCs已成功由多种体细胞诱导出来，不仅具有发育多能性还能避免胚胎干细胞(Embryonic stem cells, ESCs)的伦理道德问题，已成为生命科学领域不可或缺的研究工具，具有广阔的应用前景。但获得高质量、遗传稳定的iPSCs是当前亟须解决的问题。文章对iPSCs重编程机制和遗传稳定性的研究进展进行了综述，以期为提高iPSCs的诱导效率、降低诱导成本、掌握iPSCs质量控制的关键点提供参考，从而推进多能性干细胞临床应用的发展。

关键词: iPSCs, 遗传稳定性, 重编程机制, 表观遗传学

Abstract: Induced pluripotent stem cells (iPSCs) were reprogrammed from somatic cells using specific transcription factors. Bypassing the ethical issue caused by embryonic stem cells (ESCs), iPSCs can be successfully induced from a variety of cells, which makes iPSCs a powerful research tool for developmental biology. iPSCs have also become indispensable to the research of life science due to their broad potential applications. However, it's a big challenge to obtain iPSCs with high quality and genetic stability. Here, we review the research progress of increasing the reprogramming mechanism and genetic stability of iPSCs in order to provide references of reprogramming efficiency of iPSCs, reducing the cost, and addressing key points of iPSCs quality control, further promotingclinical application of the iPSCs.

Key words: iPSCs, genetic stability, reprogramming, epigenetics

任才芳，孙红艳，王立中，张国敏，樊懿萱，颜光耀，王丹，王锋. iPSCs遗传稳定性与重编程机制的研究进展[J]. 遗传, 2014, 36(9): 879-887.

Caifang Ren, Hongyan Sun, Lizhong Wang, Guomin Zhang, Yixuan Fan, Guangyao Yan, Dan Wang, Feng Wang. Reprogramming mechanism and genetic stability of induced pluripotent stem cells (iPSCs)[J]. HEREDITAS(Beijing), 2014, 36(9): 879-887.

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iPSCs遗传稳定性与重编程机制的研究进展

Reprogramming mechanism and genetic stability of induced pluripotent stem cells (iPSCs)

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