遗传 ›› 2015, Vol. 37 ›› Issue (8): 828-836.doi: 10.16288/j.yczz.15-032

• 研究报告 • 上一篇    下一篇

绿色杜氏藻转录组分析

朱帅旗, 龚一富, 杭雨晴, 刘浩, 王何瑜   

  1. 宁波大学海洋学院,宁波 315211
  • 收稿日期:2015-01-14 修回日期:2015-03-25 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: 龚一富,博士,副教授,研究方向:植物生化与分子。E-mail: gongyifu@163.com
  • 作者简介:朱帅旗,硕士研究生,专业方向:藻类生物化学与分子生物学。E-mail: ljlzj2009@163.com
  • 基金资助:
    浙江省科技厅重点科技创新团队项目(编号:2012R10029-07,2010R50029)和宁波科技攻关项目(编号:2013C10018)资助

Transcriptome analysis of Dunaliella viridis

Shuaiqi Zhu, Yifu Gong, Yuqing Hang, Hao Liu, Heyu Wang   

  1. School of Marine Sciences, Ningbo University, Ningbo 315211, China
  • Received:2015-01-14 Revised:2015-03-25 Online:2015-08-20 Published:2015-08-20

摘要: 为了深入了解绿色杜氏藻(Dunaliella viridis)基因信息及功能、耐盐相关通路(甘油脂代谢)及关键酶,本文首次通过Illumina HiSeqTM 2000高通量测序技术对绿色杜氏藻转录组进行测序,利用Trinity软件将数据组装形成转录本,对所有转录本进行COG(Clusters of Orthologous Groups)、GO(Gene Ontology)和KEGG(Kyoto Encyclopedia of Genes and Genomes)分类和功能注释、Pathway注释以及蛋白编码区(Opening reading fragment,ORF)的预测,并对甘油脂代谢通路关键酶基因进行了分析。转录组测序共获得81 593个转录本,其中ORF共有77 117条,约占所有转录本的94.50%。COG分类结果表明,16 569条转录本被分为24个类别。GO分类结果表明,76 436条转录本被注释。在所有注释分类中,生物学过程转录本数量最多,为30 678条,占总转录本数的40.14%。KEGG分析结果表明,317个标准途径中包含26 428条转录本,含转录本最多的类别是代谢,为9949条(37.65%)。与代谢有关的途径为131条,占所有注释途径的41.32%。在甘油脂代谢通路中仅发现1条关键酶转录本(二羟丙酮激酶),该酶可能与绿色杜氏藻耐盐胁迫中甘油的合成有较大关系。本研究进一步完善了绿色杜氏藻的基因信息,为绿色杜氏藻代谢途径研究奠定了坚实的基础。

关键词: 绿色杜氏藻, 转录组, 注释, 分类, 甘油

Abstract: In order to understand the gene information, function, haloduric pathway (glycerolipid metabolism) and related key genes for Dunaliella viridis, we used Illumina HiSeqTM 2000 high-throughput sequencing technology to sequence its transcriptome. Trinity soft was used to assemble the data to form transcripts. Based on the Clusters of Orthologous Groups (COG), Gene Ontology (GO ) and Kyoto Encyclopedia of Genes and Genomes (KEGG ) databases, we carried out functional annotation and classification, pathway annotation, and the opening reading fragment (ORF) sequence prediction of transcripts. The key genes in the glycerolipid metabolism were analyzed. The results suggested that 81 593 transcripts were found, and 77 117 ORF sequences were predicted, accounting for 94.50% of all transcripts. COG classification results showed that 16 569 transcripts were assigned to 24 categories. GO classification annotated 76 436 transcripts. The number of transcripts for biologcial processes was 30 678, accounting for 40.14% of all transcripts. KEGG pathway analysis showed that 26 428 transcripts were annotated to 317 pathways, and 131 pathways were related to metabolism, accounting for 41.32% of all annotated pathways. Only one transcript was annotated as coding the key enzyme dihydroxyacetone kinase involved in the glycerolipid pathway. This enzyme could be related to glycerol biosynthesis under salt stress. This study further improved the gene information and laid the foundation of metabolic pathway research for Dunaliella viridis.

Key words: Dunaliella viridis, transcriptome, annotation, classification, glycerol