组蛋白泛素化修饰及其在DNA损伤应答中的作用

doi:10.16288/j.yczz.18-112

遗传 ›› 2019, Vol. 41 ›› Issue (1): 29-40.doi: 10.16288/j.yczz.18-112

组蛋白泛素化修饰及其在DNA损伤应答中的作用

张卿义¹,张樱子²,沈凯¹,张舒羽²,曹建平²

^1. 苏州大学医学部第一临床医学院,苏州 215123
^2. 苏州大学医学部放射医学与防护学院,苏州 215123

收稿日期:2018-07-10 修回日期:2018-09-04 出版日期:2019-01-20 发布日期:2018-10-19
作者简介:张卿义,本科在读,专业方向：临床医学。E-mail: zhangqingyi@outlook.com
基金资助:
国家级大学生创新创业训练计划(编号：201610285039Z,201610285045Z)资助

Histone ubiquitylation and its roles in DNA damage response

Zhang Qingyi¹,Zhang Yingzi²,Shen Kai¹,Zhang Shuyu²,Cao Jianping²

^1. The First Clinical Medical School, Soochow University, Suzhou 215123, China
^2. School of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Science, Soochow University, Suzhou 215123, China

Received:2018-07-10 Revised:2018-09-04 Online:2019-01-20 Published:2018-10-19
Supported by:
[Supported by the National Students’ Platform for Innovation and Entrepreneurship Training Program (Nos. 201610285039Z, 201610285045Z)]

摘要/Abstract

摘要：

泛素化修饰是真核生物细胞内重要的翻译后修饰类型,通过调节蛋白质活性、稳定性和亚细胞定位广泛参与细胞内各项信号传导与代谢过程,对维持正常生命活动具有重要意义。组蛋白作为染色质中主要的蛋白成分,与DNA复制转录、修复等行为密切相关,是研究翻译后修饰的热点。DNA损伤后,组蛋白泛素化修饰通过调节核小体结构、激活细胞周期检查点、影响修复因子的招募与装配等诸多途径参与损伤应答。同时,组蛋白泛素化修饰还能调节其他位点翻译后修饰,并通过这种串扰(crosstalk)作用调节DNA损伤应答。本文介绍了组蛋白泛素化修饰的主要位点和相关组分(包括E3连接酶、去泛素化酶与效应分子),以及这些修饰作用共同编译形成的信号网络在DNA损伤应答中的作用,最后总结了目前该领域研究所面临的一些问题,以期为科研人员进一步探索组蛋白密码在DNA损伤应答中的作用提供参考。

关键词: 组蛋白, 泛素化修饰, DNA损伤应答, 串扰

Abstract:

Ubiquitylation is an essential type of protein post-translational modifications (PTMs) in eukaryotes, which mediates various biological processes by regulating the subcellular localization, activity, and stability of proteins. Histones, as the main protein ingredients of chromatin, are closely coupled with DNA activities such as replication, transcription and repair, and therefore are the hotspots of PTMs. After DNA damage, histone ubiquitylations are involved in DNA damage response (DDR) by regulating nucleosome structure, activating cell cycle checkpoints, remodeling the nucleosome, and the recruitment and assembly of repair factors. Meanwhile, histone ubiquitylations can also crosstalk with other types of PTMs to regulate DDR processes. In this review, we summarize how the site-specific histone ubiquitylation forms signal network and contributes to DDR, which may shed light on the further study of how histone codes formed by histone PTMs affect the entire DDR processes.

Key words: histone, ubiquitylation, DNA damage response (DDR), crosstalk

张卿义, 张樱子, 沈凯, 张舒羽, 曹建平. 组蛋白泛素化修饰及其在DNA损伤应答中的作用[J]. 遗传, 2019, 41(1): 29-40.

Zhang Qingyi, Zhang Yingzi, Shen Kai, Zhang Shuyu, Cao Jianping. Histone ubiquitylation and its roles in DNA damage response[J]. Hereditas(Beijing), 2019, 41(1): 29-40.

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[1]	张競文,续倩,李国亮. 癌症发生发展中的表观遗传学研究[J]. 遗传, 2019, 41(7): 567-581.
[2]	甘炎民,周健,全绒,洪林君,李紫聪,郑恩琴,刘德武,吴珍芳,蔡更元,顾婷. 组蛋白H3K27me3对骨骼肌发育调控研究进展[J]. 遗传, 2019, 41(4): 285-292.
[3]	潘云枫, 王演怡, 陈静雯, 范怡梅. 线粒体代谢介导的表观遗传改变与衰老研究[J]. 遗传, 2019, 41(10): 893-904.
[4]	鞠君毅,赵权. γ-珠蛋白基因表达调控机制与临床应用[J]. 遗传, 2018, 40(6): 429-444.
[5]	黄星卫, 程香荣, 王楠, 张雨薇, 廖辰, 金连弘, 雷蕾. 组蛋白H3变体H3.3及其在细胞重编程中的作用[J]. 遗传, 2018, 40(3): 186-196.
[6]	刘辰东, 杨露, 蒲红州, 杨琼, 黄文耀, 赵雪, 朱砺, 张顺华. 运动对骨骼肌基因表达的表观遗传调控作用[J]. 遗传, 2017, 39(10): 888-896.
[7]	张轲, 冯光德, 张宝云, 向伟, 陈龙, 杨芳, 储明星, 王凭青. 表观遗传标记在猪分子育种中的研究与应用前景[J]. 遗传, 2016, 38(7): 634-643.
[8]	李建辉, 汪春付, 白帆, 庄严, 毛卓君, 孙永涛. 流式细胞术检测组蛋白乙酰化水平方法的建立与应用[J]. 遗传, 2016, 38(6): 581-587.
[9]	曹继红, 廖尉廷, 沃琤, 徐国荣, 徐焕新, 李平龙, 陶冶, 王鹏, 林加日, 邓连瑞. 组蛋白去乙酰化酶抑制剂影响的代谢相关基因的组学筛查及验证[J]. 遗传, 2015, 37(9): 918-925.
[10]	何珊, 张令强. 线性泛素化修饰研究进展[J]. 遗传, 2015, 37(9): 911-917.
[11]	孙凌云, 李星逾, 孙志为. 原发性肝癌的表观遗传学及其治疗[J]. 遗传, 2015, 37(6): 517-527.
[12]	乔晓孟,殷芳圆,李云肖,魏曙光,赖江华. 大鼠基底外侧杏仁核组蛋白乙酰化对吗啡成瘾记忆的调控作用[J]. 遗传, 2015, 37(4): 382-387.
[13]	曹丽娟,刘昕訸,查晴,宋倩,杨克,刘艳. SIRT3与细胞代谢及心血管疾病的相关性[J]. 遗传, 2015, 37(2): 111-120.
[14]	李芬, 杨敬辉, 吴秀丽, 张卫林, 王冠峰, 岳晓杰. 酿酒酵母组蛋白H3 K4L和K36L突变对细胞生长和GAL1、SSA3、PHO5表达的影响[J]. 遗传, 2014, 36(8): 827-834.
[15]	郭雨辰, 雷秉坤, 邓小龙, 余垚, 吕红. sgf73⁺在裂殖酵母中的大规模遗传筛选[J]. 遗传, 2014, 36(7): 723-731.