遗传 ›› 2018, Vol. 40 ›› Issue (8): 683-692.doi: 10.16288/j.yczz.18-151

• 资源与平台 • 上一篇    

国家斑马鱼资源中心的资源、技术和服务建设

熊凤,谢训卫,潘鲁媛,李阔宇,柳力月,张昀,李玲璐,孙永华()   

  1. 中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,国家斑马鱼资源中心,武汉 430072
  • 收稿日期:2018-05-29 修回日期:2018-07-11 出版日期:2018-08-16 发布日期:2018-07-25
  • 通讯作者: 孙永华 E-mail:yhsun@ihb.ac.cn
  • 作者简介:熊凤,博士,工程师,研究方向：斑马鱼模式动物。E-mail: xiongfeng@ihb.ac.cn
  • 基金资助:
    国家自然科学基金项目资助(31671501);国家自然科学基金项目资助(39970774);国家自然科学基金项目资助(31672378)

Development of resources, technologies and services at the China Zebrafish Resource Center

Feng Xiong,Xunwei Xie,Luyuan Pan,Kuoyu Li,Liyue Liu,Yun Zhang,Linglu Li,Yonghua Sun()   

  1. China Zebrafish Resource Center, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  • Received:2018-05-29 Revised:2018-07-11 Online:2018-08-16 Published:2018-07-25
  • Contact: Sun Yonghua E-mail:yhsun@ihb.ac.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(31671501);Supported by the National Natural Science Foundation of China(39970774);Supported by the National Natural Science Foundation of China(31672378)

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摘要：

随着我国斑马鱼研究群体的日益壮大,对各类斑马鱼研究资源和技术的需求日益增加,国家斑马鱼资源中心(China Zebrafish Resource Center, CZRC, 网址:http://zfish.cn)于2012年在中国科学院水生生物研究所成立。目前,CZRC已发展成为国内规模最大的单体斑马鱼养殖系统,建成包含1200多个斑马鱼品系和10 000余份冻存精子的斑马鱼资源保藏库,其中有超过200个突变和转基因品系是由CZRC自主创制。在此基础上,CZRC建立了安全规范的斑马鱼养殖和健康平台、高效的基因操作平台和稳定高效的精子冻存平台。CZRC致力于为国内外从事斑马鱼研究的科研人员提供各类服务,包括提供斑马鱼品系等资源服务、转基因和基因敲除等技术服务、养殖和健康等咨询服务,以及技术培训和学术会议服务等。经过5年的建设,CZRC已成为国际学术界公认的全球三大斑马鱼资源库之一。

关键词: 斑马鱼, 国家中心, 资源, 技术, 服务

Abstract:

With the rapid growth of the Chinese zebrafish community, there is an increasing demand for various types of zebrafish-related resources and technologies. The China Zebrafish Resource Center (CZRC, web: http://zfish.cn) was established at the Institute of Hydrobiology (IHB), Chinese Academy of Sciences (CAS) in 2012. Till now, CZRC has built the largest zebrafish aquaculture unit in China, organized a resource bank containing more than 1200 zebrafish lines and more than 10 000 frozen sperm samples, among which over 200 mutant and transgenic lines were generated by CZRC. CZRC has established several technical supporting platforms, such as the zebrafish husbandry and health control program of international standard, a high-efficient gene manipulation technology platform, and a stable and efficient sperm cryopreservation technology platform. The main task of CZRC is to provide different types of services to zebrafish investigators in China and worldwide, such as resource services (e.g. zebrafish lines), technical services (e.g. gene knockout) and transgenic services, consultancy services (e.g. zebrafish husbandry and health consultation), and conference services [e.g. holding regular technical training courses and biennale Chinese Zebrafish Principal Investigator Meeting (CZPM)]. After five years’ development, CZRC is now recognized as one of the three major resource centers in the global zebrafish community.

Key words: zebrafish, national center, resources, technology, services