遗传 ›› 2008, Vol. 30 ›› Issue (5): 627-632.doi: 10.3724/SP.J.1005.2008.00627

• 研究报告 • 上一篇    下一篇

兰属Cymbidium植物ISSR遗传多样性分析

吴振兴1, 王慧中1, 施农农1, 赵艳2   

  1. 1. 杭州师范大学生物化学与分子生物学杭州市重点实验室, 杭州 310018;
    2. 浙江工商大学食品、生物与环境工程学院, 杭州 310018

  • 收稿日期:2007-10-10 修回日期:2008-01-15 出版日期:2008-05-10 发布日期:2008-05-10
  • 通讯作者: 王慧中

The genetic diversity of Cymbidium by ISSR

WU Zhen-Xing1, WANG Hui-Zhong1, SHI Nong-Nong1, ZHAO Yan2   

  1. 1. Key Laboratory of Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310018, China;
    2. College of Food, Biological and Environmental Engineering, Zhejiang Gongshang University, Hangzhou 310018, China
  • Received:2007-10-10 Revised:2008-01-15 Online:2008-05-10 Published:2008-05-10
  • Contact: WANG Hui-Zhong

摘要:

将简单重复序列区间扩增多态标记(inter-simple sequence repeats, ISSR)技术应用于16种兰属的遗传多样性分析, 15个引物共扩增出836条带, 其中有227条多态带, 多态百分比为27.2%。UPGMA聚类结果显示: 春兰与春剑的亲缘关系最近, 而兔耳兰与其他15种兰属植物的距离最远。ISSR聚类结果与传统分类结果基本相似, 表明该技术能在分子水平上对传统兰属分类进行必要补充。

关键词: 兰属植物, 遗传多样性, ISSR

Abstract:

ISSR was applied to detect the relationship between 16 Cymbidium species, and 836 bands were amplified with 15 primers, including 227 polymorphic bands. The polymorphic percentage is 27.2%. UPGMA results showed that the genetic distance were closest between C.goeringii (Rchb.f.) Rchb.f. and C. goeringii var. longibracteatum, and C.lancifolium Hook. was far away from the other 15 species. This result is quite similar to the traditional classification, indicating that the technique could supplement some information to traditional taxonomy in the molecular level.