遗传 ›› 1998, Vol. 20 ›› Issue (6): 30-33.

• 论文 • 上一篇    下一篇

水稻抗白叶枯病基因Xα-14在分子标记连锁图上的定位

谭震波1;2; 章琦1; 朱立煌2; 王春莲1 TAN Zhen-bo1;2;ZHANG Qi1;ZHU Li-huang2;WANG Chun-lian1   

  1. 1.中国农业科学院作物育种栽培研究所, 北京 100081 2.中国科学院遗传所,北京新技术公司100101 1.Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Sciences,Beijing 100081; 2.Institute of Genetics,Chinese Academy of Sciences,Beijing 100101
  • 收稿日期:1900-01-01 出版日期:1998-12-10 发布日期:1998-12-10

RFLP Mapping of a Rice Bacterial Blight Resistance Gene X-α14

  • Received:1900-01-01 Online:1998-12-10 Published:1998-12-10

摘要: 选取对水稻白叶枯病原菌6个菲律宾小种均为感病的籼稻品种珍珠矮为母本,携带抗病基因Xα-14的粳稻近等基因系CBB14(抗菲律宾5号小种P5)为父本配制杂交组合。F1植株对相对应的菌系P5表现全生育期抗病, F2群体在分蘖前期接种鉴定结果表明,抗、感植株的分离符合3:1显性单基因分离比。根据对F2群体中选择的99个单株进行的RFLP分析的结果,构建了水稻第4连锁群的分子图谱,并把抗病基因Xα-14定位于RG62 0和G282之间。
Abstract:In order to map a rice bacterial blight resistance gene Xα-14,a F2 population containing 99 plants was generated from a cross between Zhenzhuai(susceptible to all six Philippines races)and CBB14 with Xα-14(resistant to race 5).It was observed that resistant and susceptible plants were segregated in a ratio of 3:1.Based on RFLP analysis in the population,a molecular linkage map of chromosome 4 was constructed,and the gene Xα-14 was located between RFLP markers RG 620 and G282.

关键词: 水稻, 白叶枯病, 基因定位, Rice, RFLP分析
Key words,
遗传多样性, Oryaz rufipogon, 核糖体RNA基因, 基因间间隔序列

Key words: Intergenic spacer, Oryza rufipogon, rDNA, Genetic diversity