遗传 ›› 2009, Vol. 31 ›› Issue (3): 325-366.doi: 10.3724/SP.J.1005.2009.00325

• 技术与方法 • 上一篇    

核酸-蛋白质互作的生物化学研究方法

张金璧;潘增祥;林飞;马雪山;刘红林   

  1. 南京农业大学动物科技学院, 南京210095
  • 收稿日期:2008-07-18 修回日期:2008-12-08 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 刘红林

Biochemical methods for the analysis of DNA-protein interactions

ZHANG Jin-Bi;PAN Zeng-Xiang;LIN Fei;MA Xue-Shan;LIU Hong-Lin   

  1. College of animal science and technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2008-07-18 Revised:2008-12-08 Online:2009-03-10 Published:2009-03-10
  • Contact: LIU Hong-Lin

摘要: 研究核酸-蛋白质的互作是揭示生命活动机理的基础, 文章简要综述了用于研究核酸-蛋白质互作的各种生物化学方法。从体内、体外两个研究角度, 针对核酸、蛋白以及复合物3个研究水平, 概述了硝化纤维膜过滤实验、足迹法、EMSA、Southwestern杂交等经典分析方法的原理、发展和运用。还着重介绍了最近在表观遗传学领域中广泛运用的nChIP、xChIP等基本染色质免疫沉淀(ChIP)技术及其衍生出的ChIP-on-chip等方法。

关键词: 互作, 足迹, 染色质免疫沉淀, 核酸, 蛋白质

Abstract: Investigation of DNA-protein interactions is fundamental to understand the mechanism underlying a variety of life processes. In this article, various types of biochemical methods in DNA-protein interaction study in vivo and in vitro at the level of DNA, protein, and the complex, respectively were briefly reviewed. Traditional assays including Nitrocellulose filter-binding assay, Footprinting, EMSA, and Southwestern blotting were summarized. In addition, chromatin immunopre-cipitation techniques including nChIP, xChIP, and ChIP-on-chip, which were widely used in epigenetics, were particularly introduced.