水稻突触融合相关蛋白基因(OsKNOLLE)在酵母中的异源表达及在非生物胁迫中的作用初探

doi:10.3724/SP.J.1005.2011.01251

遗传 ›› 2011, Vol. 33 ›› Issue (11): 1251-1257.doi: 10.3724/SP.J.1005.2011.01251

水稻突触融合相关蛋白基因(OsKNOLLE)在酵母中的异源表达及在非生物胁迫中的作用初探

王飞娟, 朱诚

中国计量学院生命科学学院, 浙江省生物计量及检验检疫技术重点实验室, 杭州310018

收稿日期:2011-03-17 修回日期:2011-06-18 出版日期:2011-11-20 发布日期:2011-11-25
通讯作者: 朱诚 E-mail:pzhuch@zju.edu.cn
基金资助:
浙江省自然科学基金项目(编号：Y3110334), 浙江省重点创新团队, 浙江省科技创新团队项目(编号: 2010R50028),“十一五”国家科技支撑计划重点项目(编号：2006BAK02A18)和国家重点基础研究发展规划(973计划)项目(编号：2002CB10804)资助

Heterologous expression of a rice syntaxin-related protein KNOLLE gene (OsKNOLLE) in yeast and its functional analysis in the role of abiotic stress

WANG Fei-Juan, ZHU Cheng

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China

Received:2011-03-17 Revised:2011-06-18 Online:2011-11-20 Published:2011-11-25
Contact: ZHU Cheng E-mail:pzhuch@zju.edu.cn

摘要/Abstract

摘要： 突触融合相关蛋白属于多功能蛋白家族SNARE(SolubleN-ethylmaleimide-sensitive factor attachment protein receptor)的亚家族蛋白, 这类家族在植物的许多生理过程中都起着非常重要的作用。为探明水稻突触融合相关蛋白OsKNOLLE(Syntaxin-related protein KNOLLE)的功能, 文章从“中花11”水稻根中克隆OsKNOLLE基因的CDS序列并构建到酵母表达载体pYX212上, 同时转化酿酒酵母菌株BY4741。通过比较不同酵母转化子在多种非生物因子胁迫下的表型, 显示转OsKNOLLE酵母菌比对照菌适应逆境能力更强, 表明OsKNOLLE与盐、Cu²⁺、H₂O₂、Cd²⁺、Hg²⁺胁迫相关, 推测OsKNOLLE在植物对逆境的耐性中发挥重要作用。本研究为OsKNOLLE基因的研究提供方法支持, 同时明确了其与不同非生物因子的相关性。

Abstract: Syntaxin-related protein KNOLLE, a multifunctional protein family belonging to the SNARE superfamily, plays an important role in many physiological processes in plants. In order to understand the function of the syntaxin-related protein KNOLLE (OsKNOLLE) in rice (Oryza sativa), the CDS sequence of OsKNOLLE gene isolated from a japonica rice cultivar “Zhonghua 11” was fused into an expression vector pYX212 and transformed into the S. cerevisiae strain BY4741 by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. The transformants with OsKNOLLE showed better survival abilities than the transformants with the empty vectors based on their phenotypes in responses to different abiotic stresses such as salt, Cu²⁺, H₂O₂, Cd²⁺, and Hg²⁺. These data suggests that OsKNOLLE plays a crucial role in responses to abiotic stresses. This experimental system sets up a method for studying functions of the OsKNOLLE gene in the future and clarifies the relationship between OsKNOLLE and abiotic stresses.

Key words: rice, syntaxin-related protein KNOLLE, heteroexpression, abiotic stress, functional analysis

中图分类号:

王飞娟，朱诚. 水稻突触融合相关蛋白基因(OsKNOLLE)在酵母中的异源表达及在非生物胁迫中的作用初探[J]. 遗传, 2011, 33(11): 1251-1257.

WANG Fei-Juan, ZHU Cheng. Heterologous expression of a rice syntaxin-related protein KNOLLE gene (OsKNOLLE) in yeast and its functional analysis in the role of abiotic stress[J]. HEREDITAS, 2011, 33(11): 1251-1257.

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水稻突触融合相关蛋白基因(OsKNOLLE)在酵母中的异源表达及在非生物胁迫中的作用初探

Heterologous expression of a rice syntaxin-related protein KNOLLE gene (OsKNOLLE) in yeast and its functional analysis in the role of abiotic stress

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