基于线粒体和核基因序列的蜜蜂属系统发育分析

doi:10.3724/SP.J.1005.2012.01057

遗传 ›› 2012, Vol. 34 ›› Issue (8): 1057-1063.doi: 10.3724/SP.J.1005.2012.01057

基于线粒体和核基因序列的蜜蜂属系统发育分析

曹联飞¹, 牛德芳¹, 和绍禹², 匡海鸥², 胡福良¹

1. 浙江大学动物科学学院, 杭州 310058 2. 云南农业大学东方蜜蜂研究所, 昆明 650201

收稿日期:2012-01-06 修回日期:2012-02-24 出版日期:2012-08-20 发布日期:2012-08-25
通讯作者: 胡福良 E-mail:flhu@zju.edu.cn
基金资助:
国家蜂产业技术体系项目(编号：CARS-45)资助

Phylogenetic relationships of honey bees (Hymenoptera: Apinae) in-ferred from mitochondrial and nuclear DNA sequences

CAO Lian-Fei¹, NIU De-Fang¹, HE Shao-Yu², KUANG Hai-Ou², HU Fu-Liang¹

1. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China 2. Eastern Bee Research Institute, Yunnan Agricultural University, Kunming 650201, China

Received:2012-01-06 Revised:2012-02-24 Online:2012-08-20 Published:2012-08-25

摘要/Abstract

摘要： 文章测定了中国分布的蜜蜂属(Apis)5种蜜蜂22个样本的线粒体基因ND2、CO2、16S rRNA以及核基因ITPR的序列, 对序列的碱基组成和蜜蜂种间的遗传距离进行了分析。结合下载的蜜蜂属其他4个种的相关序列, 采用最大简约法、邻接法和最大似然法重建了蜜蜂属系统发育关系。系统发育分析结果支持蜜蜂属划分为3个类群, 即小蜜蜂类群(包括小蜜蜂和黑小蜜蜂)、大蜜蜂类群(包括大蜜蜂和黑大蜜蜂)和穴居蜜蜂类群(西方蜜蜂、东方蜜蜂、沙巴蜂、苏拉威西蜂、绿努蜂), 且小蜜蜂类群较早分化。结果还显示, 我国海南岛的大蜜蜂和大陆的大蜜蜂之间可能存在较大的遗传分歧。

关键词: 蜜蜂属, ND2基因, CO2基因, 16S rRNA基因, ITPR基因, 系统发育

Abstract: The sequences of mitochondrial ND2, CO2, and 16S rRNA genes and nuclear ITPR gene were obtained from 22 samples of 5 Apis species from China. The characteristics of the sequences and the pairwise distances among species were analyzed. Phylogenetic trees were reconstructed for Apis species using maximum parsimony, neighbor-joining and maximum likelihood methods together with the sequences of the other 4 Apis species downloaded from GenBank. Results supported that Apis species were divided into three major clusters: dwarf bees (A. florea and A. andreniformis), giant bees (A. dorsata and A. laboriosa), and cavity-nesting bees (A. mellifera, A. cerana, A. koschevnikovi, A. nigrocinta, and A. nuluensis). The dwarf honey bees were confirmed as basal lineage. Our study also revealed a high level of genetic divergence between A. dorsata from Hainan Island and China mainland.

Key words: Apis, ND2, CO2, 16S rRNA, ITPR, molecular phylogeny

曹联飞，牛德芳，和绍禹，匡海鸥，胡福良. 基于线粒体和核基因序列的蜜蜂属系统发育分析[J]. 遗传, 2012, 34(8): 1057-1063.

CAO Lian-Fei, NIU De-Fang, HE Shao-Yu, KUANG Hai-Ou, HU Fu-Liang. Phylogenetic relationships of honey bees (Hymenoptera: Apinae) in-ferred from mitochondrial and nuclear DNA sequences[J]. HEREDITAS, 2012, 34(8): 1057-1063.

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基于线粒体和核基因序列的蜜蜂属系统发育分析

Phylogenetic relationships of honey bees (Hymenoptera: Apinae) in-ferred from mitochondrial and nuclear DNA sequences

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