遗传 ›› 2013, Vol. 35 ›› Issue (2): 175-184.doi: 10.3724/SP.J.1005.2013.00175

• 研究报告 • 上一篇    下一篇

西藏牦牛微卫星DNA的遗传多样性

李铎1, 柴志欣1, 姬秋梅2, 张成福2, 信金伟2, 钟金城1   

  1. 1. 西南民族大学, 动物遗传育种学国家民委-教育部重点实验室, 成都 2. 西藏自治区农牧科学院畜牧兽医研究所, 拉萨 850009
  • 收稿日期:2012-10-04 修回日期:2012-11-26 出版日期:2013-02-20 发布日期:2013-02-25
  • 通讯作者: 钟金城 E-mail:zhongjincheng518@126.com
  • 基金资助:

    国家科技支撑计划课题资助(编号：2012BAD03B02)和西南民族大学研究生学位点建设项目(编号：2011XWD-S071007)资助

Genetic diversity of DNA microsatellite for Tibetan Yak

LI Duo1, CHAI Zhi-Xin1, JI Qiu-Mei2, ZHANG Cheng-Fu2, XIN Jin-Wei2, ZHONG Jin-Cheng1   

  1. 1. Key Laboratory of Animal Genetics and Breeding, SouthwestUniversity for Nationalities, State Ethnic Affairs Commission and Ministry of Education, Chengdu 610041, China 2. Institute of Animal Scienceand Veterinary, Academy of Agricultural Sciences, Tibet Autonomous Region, Lhasa 850009, China
  • Received:2012-10-04 Revised:2012-11-26 Online:2013-02-20 Published:2013-02-25

PDF (PC)

1744

被引次数

31

摘要： 为了解西藏牦牛品种或类群的遗传多样性和亲缘关系, 文章选用8对微卫星标记引物, 利用PCR和复合电泳银染技术, 通过计算基因频率(P)、有效等位基因数(Ne)、群体杂合度(He)、多态信息含量(PIC)和遗传距离(D), 对西藏11个牦牛类群共480个个体进行了遗传多样性和系统进化分析。结果表明：(1) 8个微卫星标记在西藏牦牛类群中均表现出多态性, 且均属高度多态位点, 遗传多样性丰富; (2) 8个微卫星标记的平均多态信息含量在西藏牦牛类群中均高于0.5, 其中HEL13最高为0.8496, TGLA57最低为0.7349; 在11个牦牛类群中, 桑日牦牛的平均多态信息含量最高(0.7949), 该群体内部存在较多的遗传变异; 丁青牦牛最低(0.7505), 则群体相对较纯; (3) 在11个牦牛类群中, 其杂合度大小分别为：桑日(0.8193)>江达(0.8190)>桑桑(0.8157)>巴青(0.8150)> 康布(0.8123)>嘉黎(0.8087)>工布江达(0.8054)>斯布(0.8041)>类乌齐(0.8033)>帕里(0.8031)>丁青(0.7831), 西藏东部牦牛的遗传多样性较西部的遗传多样性大, 预示西藏东部可能是牦牛的发源地之一; (4) 根据遗传距离, 用UPGMA法构建聚类关系, 表明西藏11个牦牛类群可以分为三大类, 即嘉黎牦牛、帕里牦牛、桑桑牦牛、巴青牦牛、类乌齐牦牛、康布牦牛聚为一类, 斯布牦牛、工布江达牦牛、桑日额牛、江达牦牛聚为一类, 丁青牦牛单独成为一类。综上所述, 西藏牦牛的遗传多样性较丰富, 所选微卫星标记可用于西藏牦牛遗传多样性的评估。

关键词: 西藏牦牛, 微卫星, 遗传多样性

Abstract: To assess the genetic diversity and relationship of the Tibetan yak breeds. The genetic diversity and phylogenies of a total of 480 individual from 11 Tibetan yak groups were analyzed using PCR and multiplex gel electrophoresis of silver staining technology with eight pairs of microsatellite markers.The result showed that these markers were highly polymorphic loci with rich genetic diversity in the Tibetan yak populations.The average polymorphic information content (PIC) in 11 groups of yak were higher than 0.5. The highest HEL13 was 0.8496, and the lowest TGLA57 was 0.7349. Among them, the PIC of Dingqing yak was minimum (0.7505), indicating that the group is relatively pure.Sangri Yak had the highest PIC value (0.7949) indicating greater genetic variationwithinthe groups. Among the 11 groups examined, the order of heterozygosity size wasSan-gri(0.8193)>Jiangda(0.8190)>Sangsang(0.8157)>Baqing(0.8150)>Kangbu(0.8123)>Jiali(0.8087)>Gongbujiangda(0.8054)>Sibu(0.8041)>Leiwuqi(0.8033)>Pali(0.8031)>Dingqing(0.7831). The groups from eastern Tibet had grater genetic diversity than those from Western Tibet, which indicate that Tibet may be one of the cradles of the yak.According to the genetic distance, the cluster relationship constructed with UPGMA and NJ methods showed that 11 yak groups in Tibet could be divided into three forms. In summary,Tibet yak has abundant genetic diversity and the selected microsatellite markers can be used to evaluategenetic diversity of Tibetan yak.

Key words: Tibetan Yak, microsatellite loci, genetic diversity

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

版权所有 © 中国科学院遗传与发育生物学研究所 中国遗传学会
地址：北京市朝阳区北辰西路1号院　中国科学院遗传与发育生物学研究所1号楼　
邮政编码：100101
联系电话：010-64807669
E-mail：yczz@genetics.ac.cn