遗传 ›› 2013, Vol. 35 ›› Issue (3): 262-268.doi: 10.3724/SP.J.1005.2013.00262

• 综述 • 上一篇    下一篇

细胞抽提物诱导的体细胞重编程

范宗兴, 朱化彬, 杜卫华   

  1. 中国农业科学院北京畜牧兽医研究所, 胚胎工程与繁殖技术研究室, 北京 100193
  • 收稿日期:2012-08-29 修回日期:2012-10-09 出版日期:2013-03-20 发布日期:2013-03-25
  • 通讯作者: 杜卫华 E-mail:dwh679@sohu.com
  • 基金资助:

    科技支撑项目(编号:2012BAD12B01)和2013的基本科研业务费项目(编号: 2013ywf-2d-2)资助

Somatic cells reprogramming using cell extracts

FAN Zong-Xing, ZHU Hua-Bin, DU Wei-Hua   

  1. Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2012-08-29 Revised:2012-10-09 Online:2013-03-20 Published:2013-03-25

摘要: 体细胞重编程是指在特定条件下将已分化的体细胞去分化逆转回原始多能状态或转分化为其他类型细胞。目前诱导体细胞重编程的方法主要有:体细胞核移植、细胞融合、特定转录因子转染、细胞抽提物诱导等。近年来抽提物诱导体细胞重编程越来越受到研究者的关注。利用此法, 通过重编程不仅可以获得需要类型的细胞, 而且方便识别与重编程有关的细胞因子, 探求重编程的机制。文章简略概述诱导体细胞重编程的方法, 并重点阐述细胞抽提物诱导体细胞重编程的研究进展。

关键词: 抽提物, 重编程, 体细胞核移植

Abstract: The process of differentiated somatic cells to a pluripotent state or to another unrelated cell type is referred to as cellular reprogramming. This can be achieved through somatic cell nuclear transfer, cell fusion, specific transcription-factor transduction, and exposure to cell extracts. Cellular reprogramming using cell extracts has gained increasing attention in recent years and has been applied to acquire the target cell types, recognize the functional factors, explore the reprogramming mechanism. In this review, we described different methods of reprogramming somatic cells, which mainly focused on the advances in cellular reprogramming using cell extracts.

Key words: extracts, reprogramming, somatic cell nuclear transfer