遗传 ›› 2011, Vol. 33 ›› Issue (10): 1113-1120.doi: 10.3724/SP.J.1005.2011.01113

• 综述 • 上一篇    下一篇

噬菌体展示技术系统发展进展

孟繁梅, 张朝辉, 艾云灿   

  1. 中山大学生命科学学院, 有害生物控制与资源利用国家重点实验室, 广州 510275
  • 收稿日期:2011-05-04 修回日期:2011-07-31 出版日期:2011-10-20 发布日期:2011-10-25
  • 通讯作者: 艾云灿 E-mail:Lssayc@mail.sysu.edu.cn
  • 基金资助:

    国家高技术研究发展计划(863计划)项目(编号:2006AA09Z420), 广东省科技计划重点项目(编号:2005B20501001)资助

Advances of development of phage display systems

MENG Fan-Mei, ZHANG Chao-Hui, AI Yun-Can   

  1. State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2011-05-04 Revised:2011-07-31 Online:2011-10-20 Published:2011-10-25

摘要: 噬菌体展示技术(Phage display technology, PDT)是一种特殊的基因工程重组表达技术, 噬菌体展示技术系统(Phage display system, PDS)是指包括经过遗传改造后的系列噬菌体、辅助噬菌体、宿主细菌等集成平台(含试剂盒)。文章从噬菌体分子遗传学及其基因(基因组)遗传工程改良角度, 基于噬菌体M13、λ、T4和T7等4大类典型噬菌体展示技术系统的发展进展进行了综述。重点强调不同展示系统中的核心部件及其基因工程改造的分子遗传学原理、不同展示锚定位点的技术特征、相关试剂盒的研制状况及选择依据。

关键词: 噬菌体, 噬菌体展示, 蛋白质表达, 基因工程, 基因组工程

Abstract: The phage display technology (PDT) was unique in genetic engineering and recombinant expression. The phage display systems (PDS) were platforms (kits) composed of genetic modified phages, helper phages, and host bacteria. This review concisely summarized the development of four types of PDS, based on M13, λ, T4, and T7 phages, in terms of phage molecular genetics and genetic (gene or genome) engineering. We addressed on the key components and their genetic (genomic) engineering for modifications, the technical features of different anchors, and the development progress and se-lection reference of those different kits.

Key words: phage, phage display, protein expression, genetic engineering, genomic engineering