Dicer调节生殖功能的研究进展

doi:10.16288/j.yczz.16-042

遗传 ›› 2016, Vol. 38 ›› Issue (7): 612-622.doi: 10.16288/j.yczz.16-042

Dicer调节生殖功能的研究进展

符梅¹, 徐克惠², 许文明²

1. 成都中医药大学,成都611137 2. 四川大学华西第二医院,四川大学–香港中文大学生殖医学联合实验室,教育部出生缺陷与相关妇儿疾病重验室,成都 610041;

收稿日期:2016-01-24 出版日期:2016-07-20 发布日期:2016-07-20
作者简介:符梅,硕士研究生,专业方向：妇产科学。E-mail: 497783767@qq.com
基金资助:
国家重点基础研究计划(973计划) (编号：2012CB944903)和新世纪人才计划项目(编号：NCET-12-0382)资助[Supported by the National Basic Research Program of China (No; 2012CB944903) and the Program for New Century Excellent Talents in University of Ministry of Education(No; NCET-12-0382)]

Research advances of Dicer in regulating reproductive function

Mei Fu², Kehui Xu¹, Wenming Xu¹

1. Joint Laboratory for Reproductive Medicine, SCU-CUHK; Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China;
2. Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China

Received:2016-01-24 Online:2016-07-20 Published:2016-07-20

摘要/Abstract

摘要： Dicer是微小非编码RNA生成的关键内切酶,介导微小RNA(micro RNA,miRNA)和小干扰RNA(small interfering RNA,siRNA)的产生,通过RNA干扰(RNA interference,RNAi)途径实现转录或转录后水平基因调控,在调节细胞增殖、分化、凋亡等方面起重要作用。近年来Dicer基因在生殖领域的研究越来越受关注,最近的研究表明Dicer与男性生精细胞发育、精子形成及成熟、精子活力和形态生成、卵泡发育、排卵及黄体形成、性激素合成、输卵管功能、子宫内膜容受性等方面都有密切关系。繁衍后代需要精子和卵子的共同参与,Dicer可能通过影响精子和卵子的数量或者质量进而导致胚胎发育异常,因此理解Dicer在雄性与雌性生殖的重要调节作用对于理解生殖调节异常相关的疾病如无精子症、复发性流产等的发病机制具有重要的作用。本文对Dicer在雄性生殖道与雌性生殖中的关键作用进行了综述,旨在进一步从分子层面深入理解Dicer与生殖相关疾病的关系。

关键词: miRNA, Dicer, 精子生成, 卵母细胞, 胚胎发育, 基因多态性

Abstract: Dicer, an RNAse III endonuclease, is critical for the biogenesis of small noncoding RNAs (microRNAs), including the biogenesis of microRNAs and small interfering RNAs, which transcriptionally and post-transcription ally regulate mRNA expression through binding to target mRNA and leading to subsequent mRNA degradation. Recent studies show that Dicer plays important roles in cell proliferation, differentiation and apoptosis. It has been attracted more and more attention in the reproductive field. In the male reproduction field, mouse model shows that Dicer is critical for the development of spermatogenic cell, sperm maturation, sperm motility and morphology. On the other hand, Dicer is broadly involved in not only follicular development, ovulation, luteinization, sex hormone synthesis, but also regulating the functions of the fallopian tube, endometrial receptivity in female reproduction. Since sperm and egg are the only two types of gametes for producing offspring, Dicer dysregulation may be the underlying cause of compromised embryo development through affecting the quantity or quality of sperm and eggs. Therefore, understanding the function of Dicer in reproduction of female and male is of great significance to study the pathogenetic mechanism related to dysfunctional reproduction, such as azoospermia and recurrent spontaneous abortion. We review the pivotal roles of Dicer in the male and female reproduction field in order to understand the relationship between Dicer and related disease.

Key words: miRNA, Dicer, spermatogenesis, oocyte, embryonic development, genetic polymorphism

符梅, 徐克惠, 许文明. Dicer调节生殖功能的研究进展[J]. 遗传, 2016, 38(7): 612-622.

Mei Fu, Kehui Xu, Wenming Xu. Research advances of Dicer in regulating reproductive function[J]. HEREDITAS(Beijing), 2016, 38(7): 612-622.

参考文献

[1] Kurzynska-Kokorniak A, Koralewska N, Pokornowska M, Urbanowicz A, Tworak A, Mickiewicz A, Figlerowicz M. The many faces of Dicer: the complexity of the mechanisms regulating Dicer gene expression and enzyme activities. Nucleic Acids Res , 2015, 43(9): 4365-4380.
[2] Hill DA, Ivanovich J, Priest JR, Gurnett CA, Dehner LP, Desruisseau D, Jarzembowski JA, Wikenheiser-Brokamp KA, Suarez BK, Whelan AJ, Williams G, Bracamontes D, Messinger Y, Goodfellow PJ. DICER1 mutations in familial pleuropulmonary blastoma. Science , 2009, 325(5943): 965.
[3] Foulkes WD, Priest JR, Duchaine TF. DICER1: mutations, microRNAs and mechanisms. Nat Rev Cancer , 2014, 14(10): 662-672.
[4] Peng JJ, Yan F, Chen HR, Chen JP. Progress of studies on Dicer structure and function. Hereditas(Beijing) , 2008, 30(12): 1550-1556. 彭杰军, 燕飞, 陈海如, 陈剑平. Dicer 结构和功能研究进展. 遗传, 2008, 30(12): 1550-1556.
[5] Maatouk DM, Loveland KL, McManus MT, Moore K, Harfe BD. Dicer1 is required for differentiation of the mouse male germline. Biol Reprod , 2008, 79(4): 696-703.
[6] Murchison EP, Stein P, Xuan ZY, Pan H, Zhang MQ, Schultz RM, Hannon GJ. Critical roles for Dicer in the female germline. Genes Dev , 2007, 21(6): 682-693.
[7] Bernstein E, Kim SY, Carmell MA, Murchison EP, Alcorn H, Li MZ, Mills AA, Elledge SJ, Anderson KV, Hannon GJ. Dicer is essential for mouse development. Nat Genet , 2003, 35: 215-217.
[8] Forbes K, Farrokhnia F, Aplin JD, Westwood M. Dicer- dependent miRNAs provide an endogenous restraint on cytotrophoblast proliferation. Placenta , 2012, 33(7): 581-585.
[9] Zindy F, Lee Y, Kawauchi D, Ayrault O, Merzoug LB, Li Y, McKinnon PJ, Roussel MF. Dicer is required for normal cerebellar development and to restrain medulloblastoma formation. PLoS One , 2015, 10(6): e0129642.
[10] Lau PW, Guiley KZ, De N, Potter CS, Carragher B, MacRae IJ. The molecular architecture of human Dicer. Nat Struct Mol Biol , 2012, 19(4): 436-440.
[11] Chen LJ, Yang M, Chen Y, Sun HQ, Xu WM. Roles of miR-15b in endothelial cell function and their relevance to vascular diseases. Hereditas(Beijing) , 2015, 37(2): 121-127. 陈良榉, 杨明, 陈艳, 孙华钦, 许文明. miR-15b在内皮细胞的功能及其与血管相关性疾病的关系. 遗传, 2015, 37(2): 121-127.
[12] Burger K, Gullerova M. Swiss army knives: non-canonical functions of nuclear Drosha and Dicer. Nat Rev Mol Cell Biol , 2015, 16(7): 417-430.
[13] Johanson TM, Lew AM, Chong MMW. MicroRNA-independent roles of the RNase III enzymes Drosha and Dicer. Open Biol , 2013, 3(10): 130144.
[14] Björkgren I, Sipilä P. The role of Dicer1 in the male reproductive tract. Asian J Androl , 2015, 17(5): 737-741.
[15] Kaneko H, Dridi S, Tarallo V, Gelfand BD, Gelfand BD, Fowler BJ, Cho WG, Kleinman ME, Ponicsan SL, Hauswirth WW, Chiodo VA, Karikó K, Yoo JW, Lee DK, Hadziahmetovic M, Song Y, Misra SS, Chaudhuri G, Buaas FW, Braun RE, Hinton DR, Zhang Q, Grossniklaus HE, Provis JM, Madigan MC, Milam AH, Justice NL, Albuquerque RJC, Blandford AD, Bogdanovich S, Hirano Y, Witta J, Fuchs E, Littman DR, Ambati BK, Rudin CM, Chong MMW, Provost P, Kugel JF, Goodrich JA, Dunaief JL, Baffi JZ, Ambati J. DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. Nature , 2011, 471(7338): 325-330.
[16] Nakagawa A, Shi Y, Kage-Nakadai E, Mitani S, Xue D. Caspase-dependent conversion of Dicer ribonuclease into a death-promoting deoxyribonuclease. Science , 2010, 328(5976): 327-334.
[17] Li P, Zhu WJ. Advance on Dicer gene and its role in female reproduction. Chin J Med Genet , 2011, 28(3): 275-278. 李平, 朱伟杰. Dicer基因在女性生殖中的作用及研究进展. 中华医学遗传学杂志, 2011, 28(3): 275-278.
[18] Romero Y, Meikar O, Papaioannou MD, Conene B, Grey C, Weier M, Pralong F, De Massy B, Kaessmann H, Vassalli JD, Kotaja N, Nef S. Dicer1 depletion in male germ cells leads to infertility due to cumulative meiotic and spermiogenic defects. PLoS One , 2011, 6(10): e25241.
[19] Korhonen HM, Meikar O, Yadav RP, Papaioannou MD, Romero Y, Da Ros M, Herrera PL, Toppari J, Nef S, Kotaja N. Dicer is required for haploid Male germ cell differentiation in mice. PLoS One , 2011, 6(9): e24821.
[20] Zimmermann C, Romero Y, Warnefors M, Bilican A, Borel C, Smith LB, Kotaja N, Kaessm H, Nef S. Germ cell-specific targeting of DICER or DGCR8 reveals a novel role for endo-siRNAs in the progression of mammalian spermatogenesis and male fertility. PLoS One , 2014, 9(9): e107023.
[21] Hayashi K, Chuva de Sousa Lopes SM, Kaneda M, Tang FC, Hajkova P, Lao KQ, O'Carroll D, Das PP, Tarakhovsky A, Miska EA, Surani MA. MicroRNA biogenesis is required for mouse primordial germ cell development and spermatogenesis. PLoS One , 2008, 3(3): e1738.
[22] Greenlee AR, Shiao MS, Snyder E, Buaas FW, Gu TJ, Stearns TM, Sharma M, Murchison EP, Puente GC, Braun RE. Deregulated sex chromosome gene expression with male germ cell-specific loss of Dicer1 . PLoS One , 2012, 7(10): e46359.
[23] Bonache S, Algaba F, Franco E, Bassas L, Larriba S. Altered gene expression signature of early stages of the germ line supports the pre-meiotic origin of human spermatogenic failure. Andrology , 2014, 2(4): 596-606.
[24] Liu DK, Li LY, Fu HL, Li SZ, Li JM. Inactivation of Dicer1 has a severe cumulative impact on the formation of mature germ cells in mouse testes. Biochem Biophys Res Commun , 2012, 422(1): 114-120.
[25] Papaioanno MD, Pitetti JL, Ro S, Park C, Aubry F, Schaad O, Vejnar CE, Kühne F, Descombes P, Zdobnov EM, McManus MT, Guillou F, Harfe BD, Yan W, Jégou B, Nef S. Sertoli cell Dicer is essential for spermatogenesis in mice. Dev Biol , 2009, 326(1): 250-259.
[26] Kim GJ, Georg I, Scherthan H, Merkenschlager M, Guillou F, Merkenschlager M, Guillou F, Scherer G, Barrionuevo F. Dicer is required for sertoli cell function and survival. Int J Dev Biol , 2010, 54: 867-875.
[27] Papaioannou MD, Lagarrigue M, Vejnar CE, Rolland AD, Kühne F, Aubry F, Schaad O, Fort A, Descombes P, Neerman-Arbez M, Guillou F, Zdobnov EM, Pineau C, Nef S. Loss of Dicer in sertoli cells has a major impact on the Testicular proteome of mice. Mol Cell Proteomics , 2011, 10(4): M900587-MCP200.
[28] Korhonen HM, Yadav RP, Da Ros M, Chalmel F, Zimmermann C, Toppari J, Nef S, Kotaja N. DICER regulates the formation and maintenance of cell-cell junctions in the mouse seminiferous epithelium . Biol Reprod , 2015, 93(6): 139.
[29] Björkgren I, Saastamoinen L, Krutskikh A, Huhtaniemi I, Poutanen M, Sipilä P. Dicer1 Ablation in the mouse epididymis causes dedifferentiation of the epithelium and imbalance in sex steroid signaling. PLoS One , 2012, 7(6): e38457.
[30] Björkgren I, Gylling H, Turunen H, Huhtaniemi I, Strauss L, Poutanen M, Sipilä P. Imbalanced lipid homeostasis in the conditional Dicer1 knockout mouse epididymis causes instability of the sperm membrane. FASEB J , 2015, 29(2): 433-442.
[31] Friedman RC, Farh KK, Burge CB, Bartel DP. Most mammalian mRNAs are conserved targets of microRNAs. Genome Res , 2009, 19(1): 92-105.
[32] Liu HC, Tang YX, He ZY, Rosenwaks Z. Dicer is a key player in oocyte maturation. J Assist Reprod Genet , 2010, 27(9-10): 571-580.
[33] Otsuka M, Zheng M, Hayashi M, Lee JD, Yoshino O, Lin SC, Han JH. Impaired microRNA processing causes corpus luteum insufficiency and infertility in mice. J Clin Invest , 2008, 118(5): 1944-1954.
[34] Yuan SQ, Ortogero N, Wu QX, Zheng HL, Yan W. Murine follicular development requires oocyte DICER, but not DROSHA. Biol Reprod , 2014, 91(2): 39.
[35] Lei L, Jin S, Gonzalez G, Behringer RR, Woodruff TK. The regulatory role of Dicer in folliculogenesis in mice. Mol Cell Endocrinol , 2010, 315(1-2): 63-73.
[36] Tang FC, Kaneda M, O'Carroll D, Hajkova P, Barton SC, Sun YA, Lee C, Tarakhovsky A, Lao KQ, Surani MA. Maternal microRNAs are essential for mouse zygotic development. Genes Dev , 2007, 21(6): 644-648.
[37] Nagaraja AK, Andreu-Vieyra C, Franco HL, Ma L, Chen RH, Han DY, Zhu HF, Agno JE, Gunaratne PH, DeMayo FJ, Matzuk MM. Deletion of Dicer in somatic cells of the female reproductive tract causes sterility. Mol Endocrinol , 2008, 22(10): 2336-2352.
[38] Stein P, Rozhkov NV, Li F, Cárdenas FL, Davydenko O, Vandivier LE, Gregory BD, Hannon GJ, Schultz RM. Essential role for endogenous siRNAs during meiosis in mouse oocytes. PLoS Genet , 2015, 11(2): e1005013.
[39] Estella C, Herrer I, Moreno-Moya JM, Quiñonero A, Martínez S, Pellicer A, Simón C. miRNA signature and Dicer requirement during human endometrial stromal decidualization in vitro . PLoS One , 2012, 7(7): e41080.
[40] Hawkins SM, Andreu-Vieyra CV, Kim TH, Jeong JW, Hodgson MC, Chen RH, Creighton CJ, Lydon JP, Gunaratne PH, DeMayo FJ, Matzuk MM. Dysregulation of uterine signaling pathways in progesterone receptor-Cre knockout of Dicer . Mol Endocrinol , 2012, 26(9): 1552-1566.
[41] Nothnick WB, Healy C, Hong XM. Steroidal regulation of uterine miRNAs is associated with modulation of the miRNA biogenesis components exportin-5 and Dicer1. Endocrine , 2010, 37(2): 265-273.
[42] Gonzalez G, Behringer RR. Dicer is required for female reproductive tract development and fertility in the mouse. Mol Reprod Dev , 2009, 76(7): 678-688.
[43] Hong XM, Luense LJ, McGinnis LK, Nothnick WB, Christenson LK. Dicer1 is essential for female fertility and normal development of the female reproductive system. Endocrinology , 2008, 149(12): 6207-6212.
[44] Feng Y, Zou SE, Weijdegård B, Chen J, Cong Q, Fernandez-Rodriguez J, Wang L, Billig H, Shao RJ. The onset of human ectopic pregnancy demonstrates a differential expression of miRNAs and their cognate targets in the Fallopian tube. Int J Clin Exp Pathol , 2014, 7(1): 64-79.
[45] Spruce T, Pernaute B, Di-Gregorio A, Cobb BS, Merkenschlager M, Manzanares M, Rodriguez TA. An early developmental role for miRNAs in the maintenance of extraembryonic stem cells in the mouse embryo. Dev Cell , 2010, 19(2): 207-219.
[46] Yang WJ, Yang DD, Na SQ, Sandusky GE, Zhang Q, Zhao GS. Dicer is required for Embryonic Angiogenesis during mouse development. J Biol Chem , 2005, 280(10): 9330- 9335.
[47] Drake M, Furuta T, Suen KM, Gonzalez G, Liu B, Kalia A, Ladbury JE, Fire AZ, Skeath JB, Arur S. A requirement for ERK-dependent Dicer phosphorylation in coordinating oocyte-to-embryo transition in C. elegans . Dev Cell , 2014, 31(5): 614-628.
[48] Cheong AWY, Pang RTK, Liu WM, Kottawatta KSA, Lee KF, Yeung WSB. MicroRNA Let-7a and dicer are important in the activation and implantation of delayed implanting mouse embryos. Hum Reprod , 2014, 29(4): 750-762.
[49] Yuan SQ, Schuster A, Tang C, Yu T, Ortogero N, Bao JQ, Zheng HL, Yan W. Sperm-borne miRNAs and endo-siRNAs Are important for fertilization and preimplantation Embryonic development. Development , 2016, 143(4): 635-647.
[50] Cho SH, Ko JJ, Kim JO, Jeon YJ, Yoo JK, Oh J, Oh D, Kim JW, Kim NK. 3'-UTR polymorphisms in the miRNA machinery genes DROSHA, DICER1, RAN, and XPO5 are associated with colorectal cancer risk in a Korean population. PLoS One , 2015, 10(7): e0131125.
[51] Xie Y, Wang Y, Zhao Y, Guo Z. Single-nucleotide polymorphisms of microRNA processing machinery genes are associated with risk for gastric cancer. Onco Targets Ther , 2015, 8: 567-571.
[52] Cao WM, Gao Y, Yang HJ, Xie SN, Meng XL, Pan ZW, Chen ZH, Huang J, Ye WW, Shao XY, Wang XJ. Germline mutations of DICER1 in Chinese women with BRCA1/ BRCA2-negative familial breast cancer. Genet Mol Res , 2014, 13(4): 10754-10760.
[53] Lu CC, Xu MF, Wang Y, Qin YF, Du GZ, Wu W, Han XM, Ji C, Yang YL, Gu AH, Xia YK, Song L, Wang SL, Wang XR. Genetic variants in meiotic program initiation pathway genes are associated with spermatogenic impairment in a Han Chinese population. PloS One , 2013, 8(1): e53443.
[54] Jung YW, Jeon YJ, Rah H, Kim JH, Shin JE, Choi DH, Cha SH, Kim NK. Genetic variants in microRNA machinery genes are associate with idiopathic recurrent pregnancy loss risk. PLoS One , 2014, 9(4): e95803.
[55] Ma HX, Yuan H, Yuan ZY, Yu CJ, Wang RX, Jiang Y, Hu ZB, Shen HB, Chen N. Genetic variations in key microRNA processing genes and risk of head and neck cancer: a case-control study in Chinese population. PLoS One , 2012, 7(10): e47544.
[56] Rah H, Jeon YJ, Lee BE, Kim JO, Shim SH, Lee WS, Choi DH, Kim JH, Kim NK. Association of polymorphisms in microRNA machinery genes (DROSHA, DICER1, RAN, and XPO5) with risk of idiopathic primary ovarian insufficiency in Korean women. Menopause , 2013, 20(10): 1067- 1073.
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Dicer调节生殖功能的研究进展

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