KRAB型锌指蛋白的进化及在物种演化中的功能

doi:10.16288/j.yczz.16-056

遗传 ›› 2016, Vol. 38 ›› Issue (11): 971-978.doi: 10.16288/j.yczz.16-056

KRAB型锌指蛋白的进化及在物种演化中的功能

王进龙, 王建, 田春艳

军事医学科学院放射与辐射医学研究所,国家蛋白质科学中心(北京),国家蛋白质组学重点实验室,蛋白质药家工程研究中心,北京 102206

收稿日期:2016-06-07 出版日期:2016-11-20 发布日期:2016-09-09
通讯作者: 王建,博士,副研究员,研究方向：蛋白质相互作用、炎症与免疫调控。E-mail: wangj@bmi.ac.cn;田春艳,博士,副研究员,研究方向：基因的转录调控。E-mail: tiancy@bmi.ac.cn
作者简介:王进龙,硕士研究生,专业方向：生物化学与分子生物学。E-mail: wang993476@163.com
基金资助:
国家自然科学基金项目(编号：31270799),北京市自然科学基金项目(编号：5142019)和国家国际科技合作专项项目(编号：2014DFB30020)资助

Evolution of KRAB-containing zinc finger proteins and their roles in species evolution

Jinlong Wang, Jian Wang, Chunyan Tian

State Key Laboratory of Proteomics, National Center for Proteomics Science (Beijing), China National Engineering Research Center for Protein Drugs, Beijing Institute of Radiation Medicine, Beijing 102206, China

Received:2016-06-07 Online:2016-11-20 Published:2016-09-09
Supported by:
[Supported by the National Natural Science Foundation of China(No. 31270799), Beijing Municipal Natural Science Foundation(No. 5142019) and International Science & Technology Cooperation Program of China(No. 2014DFB30020)]

摘要/Abstract

摘要： C2H2型锌指蛋白家族是目前发现的哺乳动物中最大的转录/转录调控因子家族,由一小群古老的含有真核锌指结构的转录因子经过多次基因复制和功能分化演化而来。KRAB型锌指蛋白(KRAB-containing zinc finger proteins, KRAB-ZFPs)作为C2H2型锌指蛋白家族中最大的亚家族,最早出现在四足脊椎动物,并随物种的进化数量快速增长,在人类中占据C2H2型锌指蛋白的60%左右。在物种演化中,进化压力主要改变KRAB-ZFPs的DNA结合能力,而KRAB-ZFPs介导的转录抑制能力则稳定存在。同时,多种KRAB-ZFPs能够与KRAB相关蛋白1(KRAB-associated protein 1, KAP1)协同作用沉默哺乳动物中反转录元件的活性,并与之协同进化,严格限制反转录原件的跳跃能力。本文综述了KRAB-ZFPs的数量倍增、锌指结构的灵活多变、KRAB-ZFPs/KAP1的转录抑制能力和反转录元件的跳跃性在促进哺乳动物调控网络的差异、基因组稳定性的变化和物种进化中的作用,旨在进一步揭示KRAB-ZFPs在推动物种稳定演化中的特点和功能。

关键词: KRAB, 锌指, KRAB-ZFPs, 进化, KAP1, 转座子

Abstract: The C2H2 zinc finger protein family, one of the largest families of transcription factor/transcriptional regulator in mammal, arose from a small ancestral group of eukaryotic zinc finger transcription factors through many repeated gene duplications accompanied by functional divergence. As the biggest subfamily of C2H2 zinc finger protein family, Kruppel-associated box-containing zinc finger proteins (KRAB-ZFPs) appeared at the period oftetrapod, expand rapidly along with species evolution, and take about 60% of the total C2H2 zinc finger proteins in human. During species evolution, the DNA binding ability of KRAB-ZFPs is changed while the KRAB-ZFPs-mediate transcriptional repression ability maintains stable under the evolution pressure. Moreover, multiple KRAB-ZFPs function synergistically with KAP1 on transcriptional silencing of retroelements, and the coevolution between KRAB-ZFPs and target retrotransposons restrict the jumping ability of the retroelements. In this review, we summarize the roles of KRAB-ZFPs duplication, the flexibility of zinc finger structure, transcriptional repression of KRAB-ZFPs/KAP1 and retroelement “jump” in promoting the divergence in regulatory network, stable genome change and species evolution, in order to reveal the characters and functions of KRAB-ZFPs in driving species evolution stably.

Key words: KRAB, zinc finger, KRAB-ZFPs, evolution, KAP1, transposons

王进龙, 王建, 田春艳. KRAB型锌指蛋白的进化及在物种演化中的功能[J]. 遗传, 2016, 38(11): 971-978.

Jinlong Wang, Jian Wang, Chunyan Tian. Evolution of KRAB-containing zinc finger proteins and their roles in species evolution[J]. Hereditas(Beijing), 2016, 38(11): 971-978.

参考文献

[1] Collins T, Stone JR, Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol Cell Biol , 2001, 21(11): 3609-3615.
[2] Stubbs L, Sun Y, Caetano-Anolles D. Function and evolution of C 2 H 2 zinc finger arrays. Subcell Biochem , 2011, 52: 75-94.
[3] Emerson RO, Thomas JH. Adaptive evolution in zinc finger transcription factors. PLoS Genet , 2009, 5(1): e1000325.
[4] Tadepally HD, Burger G, Aubry M. Evolution of C 2 H 2 - zinc finger genes and subfamilies in mammals: species-specific duplication and loss of clusters, genes and effector domains. BMC Evol Biol , 2008, 8: 176.
[5] Tian CY, Zhang LQ, He FC. Progress in the study of KRAB zinc finger protein. Hereditas ( Beijing ), 2006, 28(11): 1451-1456. 田春艳, 张令强, 贺福初. KRAB型锌指蛋白(KZNF)的研究进展. 遗传, 2006, 28(11): 1451-1456.
[6] Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ. SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Genes Dev , 2002, 16(8): 919-932.
[7] Ivanov AV, Peng H, Yurchenko V, Yap KL, Negorev DG, Schultz DC, Psulkowski E, Fredericks WJ, White DE, Maul GG, Sadofsky MJ, Zhou MM, Rauscher FJ. PHD domain-mediated E3 ligase activity directs intramolecular sumoylation of an adjacent bromodomain required for gene silencing. Mol Cell , 2007, 28(5): 823-837.
[8] Rowe HM, Trono D. Dynamic control of endogenous retroviruses during development. Virology , 2011, 411(2): 273-287.
[9] Huntley S, Baggott DM, Hamilton AT, Tran-Gyamfi M, Yang S, Kim J, Gordon L, Branscomb E, Stubbs L. A comprehensive catalog of human KRAB-associated zinc finger genes: insights into the evolutionary history of a large family of transcriptional repressors. Genome Res , 2006, 16(5): 669-677.
[10] Birtle Z, Ponting CP. Meisetz and the birth of the KRAB motif. Bioinformatics , 2006, 22(23): 2841-2845.
[11] Kono H, Tamura M, Osada N, Suzuki H, Abe K, Moriwaki K, Ohta K, Shiroishi T. Prdm9 polymorphism unveils mouse evolutionary tracks. DNA Res , 2014, 21(3): 315-326.
[12] Liu H, Chang LH, Sun Y, Lu XC, Stubbs L. Deep vertebrate roots for mammalian zinc finger transcription factor subfamilies. Genome Biol Evol , 2014, 6(3): 510-525.
[13] Vinogradov AE. Human more complex than mouse at cellular level. PLoS One , 2012, 7(7): e41753.
[14] Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Lodén M, Talhout W, Feenstra M, Abbas B, Classen AK, van Steensel B. Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Genome Res , 2006, 16(12): 1493-1504.
[15] Westphal T, Reuter G. Recombinogenic effects of suppressors of position-effect variegation in Drosophila. Genetics , 2002, 160(2): 609-621.
[16] Cam HP, Sugiyama T, Chen ES, Chen X, FitzGerald PC, Grewal SIS. Comprehensive analysis of heterochromatin-and RNAi-mediated epigenetic control of the fission yeast genome. Nat Genet , 2005, 37(8): 809-819.
[17] Vissing H, Meyer WK, Aagaard L, Tommerup N, Thiesen HJ. Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins. FEBS Lett , 1995, 369(2-3): 153-157.
[18] Lupo A, Cesaro E, Montano G, Zurlo D, Izzo P, Costanzo P. KRAB-zinc finger proteins: a repressor family displaying multiple biological functions. Curr Genomics , 2013, 14(4): 268-278.
[19] Urrutia R. KRAB-containing zinc-finger repressor proteins. Genome Biol , 2003, 4(10): 231.
[20] Emerson RO, Thomas JH. Gypsy and the birth of the SCAN domain. J Virol , 2011, 85(22): 12043-12052.
[21] King M, Wilson A. Evolution at two levels in humans and chimpanzees. Science , 1975, 188(4184): 107-116.
[22] Nadimpalli S, Persikov AV, Singh M. Pervasive variation of transcription factor orthologs contributes to regulatory network evolution. PLoS Genet , 2015, 11(3): e1005011.
[23] Looman C, Åbrink M, Mark C, Hellman L. KRAB zinc finger proteins: an analysis of the mo

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

KRAB型锌指蛋白的进化及在物种演化中的功能

Evolution of KRAB-containing zinc finger proteins and their roles in species evolution

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	王承贤, 容益康, 崔敏. 果蝇限制端粒转座子的分子机制[J]. 遗传, 2023, 45(3): 221-228.
[2]	姜明亮, 郎红, 李晓楠, 祖野, 赵靖, 彭沈凌, 刘振, 战宗祥, 朴钟云. 植物孤基因研究进展[J]. 遗传, 2022, 44(8): 682-694.
[3]	巴恒星, 胡鹏飞, 李春义. 鹿科动物基因组学研究进展[J]. 遗传, 2021, 43(4): 308-322.
[4]	吕孟冈, 刘艾嘉, 李庆伟, 苏鹏. RHR转录因子家族起源、功能以及进化机制的研究进展[J]. 遗传, 2021, 43(3): 215-225.
[5]	章誉兴, 吴宏, 于黎. 哺乳动物毛色调控机制及其适应性进化研究进展[J]. 遗传, 2021, 43(2): 118-133.
[6]	罗鑫, 宿兵. 三维基因组分析点亮人类大脑进化之谜[J]. 遗传, 2021, 43(2): 105-107.
[7]	朱医高, 李军, 逄越, 李庆伟. 七鳃鳗：生物进化和疾病研究的重要模式动物[J]. 遗传, 2020, 42(9): 847-857.
[8]	毛洋, 丁寄葳, 陈淑敏, 岑山, 李晓宇. SLFN14抗LINE-1分子机制研究[J]. 遗传, 2020, 42(7): 669-679.
[9]	胡风越, 王克剑. STEME系统：一种助力体内定向进化的新工具[J]. 遗传, 2020, 42(3): 231-235.
[10]	唐连超, 谷峰. CRISPR-Cas基因编辑系统升级：聚焦Cas蛋白和PAM[J]. 遗传, 2020, 42(3): 236-249.
[11]	梅志超, 位竹君, 于佳慧, 冀凤丹, 解莉楠. 多组学关联分析揭示表观等位基因在拟南芥环境适应性进化中的作用及机制[J]. 遗传, 2020, 42(3): 321-331.
[12]	彭威, 冯蒙洁, 陈皓, 韩宝瑜. 双翅目昆虫基因组研究进展[J]. 遗传, 2020, 42(11): 1093-1109.
[13]	何超,沈文龙,李平,张彦,曾晶,殷作明,赵志虎. Alu元件在染色质三维结构层次上的生物信息学分析[J]. 遗传, 2019, 41(3): 254-261.
[14]	孟玉,杨若林. 基于基因家族大小的比较研究脊椎动物的适应性进化[J]. 遗传, 2019, 41(2): 158-174.
[15]	汪德州,莫晓婷,张霞,徐妙云,赵军,王磊. 玉米逆境响应相关转录因子ZmC2H2-1基因克隆及功能验证[J]. 遗传, 2018, 40(9): 767-778.