遗传 ›› 2008, Vol. 30 ›› Issue (9): 1201-1206.doi: 10.3724/SP.J.1005.2008.01201

• 研究报告 • 上一篇    下一篇

水稻白色中脉Oswm2的遗传分析与分子标记定位

胡景涛1,2,3; 张甲3; 李园园1,2; 付崇允1,2; 郑静1,2; 陈家彬1,2; 胡燕1,2; 李仕贵1,2

  

  1. 1. 四川农业大学水稻研究所, 温江 611130;
    2. 四川农业大学作物基因资源与遗传改良教育部重点实验室, 雅安 625014;
    3. 重庆三峡农业科学研究所, 重庆 404001

  • 收稿日期:2008-01-09 修回日期:2008-03-13 出版日期:2008-09-10 发布日期:2008-09-10
  • 通讯作者: 李仕贵

Genetic analysis and mapping of a rice white midrib mutant Oswm2

HU Jing-Tao1,2,3; ZHANG Jia3; LI Yuan-Yuan1,2; FU Chong-Yun1,2; ZHENG Jing1,2; CHEN Jia-Bin1,2; HU Yan1,2; LI Shi-Gui1,2   

  1. 1. Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China;
    2. Key Laboratory of Ministry of Education of Crop Genetic Resource and Improvement, Sichuan Agricultural University, Ya’an 625014, China;
    3. Sanxia Institute of Agricultural Science in Chongqing, Chongqing 404001, China
  • Received:2008-01-09 Revised:2008-03-13 Online:2008-09-10 Published:2008-09-10

摘要:

从T-DNA突变体库中获得一份以中花11为遗传背景的白色中脉突变体。该突变体剑叶以下叶片的中下部中脉表现为白色, 白色中脉附近的叶色微黄, 并且伴随株高等农艺性状的改变, 暂时将其定名为Oswm2(Oryza sativa white midrib 2)。遗传分析表明该突变性状受一对隐性单基因控制, 以Oswm2和粳稻02428杂交的F2分离群体作为定位群体, 将OsWM2基因定位在水稻第7染色体的SSR标记RM21478和RM418之间, 遗传距离分别为8.7和15.9 cM。

关键词: 白色中脉, 遗传分析, 基因定位, 突变体, 水稻

Abstract:

A rice (Oryza sativa L.) plant with white midrib (Oswm2) was selected from the T-DNA inserted mutant pool of rice. The basal midrib of the leaves in the mutant, except for the flag leaves, is white. The blade close to the white midrib is etiolated, and alterations of the agronomic traits, such as plant height, panicle length occur in the mutant. Genetic analysis indicated that this mutant trait was controlled by a recessive nuclear gene. To map the OsWM2 gene, an F2 population was constructed by crossing mutant Oswm2 with 02428. The OsWM2 locus was preliminarily located between the SSR molecular markers RM21478 and RM418 on chromosome 7 with the distances of 8.7 and 15.9 cM, respectively.