鱼类核移植与重编程

doi:10.3724/SP.J.1005.2013.00433

遗传 ›› 2013, Vol. 35 ›› Issue (4): 433-440.doi: 10.3724/SP.J.1005.2013.00433

鱼类核移植与重编程

王学耕¹, 朱作言¹, 孙永华², 赵珏¹

1. 北京大学生命科学学院, 北京 100871 2. 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉 430072

收稿日期:2012-06-18 修回日期:2012-09-12 出版日期:2013-04-20 发布日期:2013-04-25
通讯作者: 赵珏 E-mail:zhaojue@pku.edu.cn
基金资助:
斑马鱼细胞核移植发育再程序化研究项目(编号：30950001)资助

Nuclear transfer and reprogramming in fish

WANG Xue-Geng¹, ZHU Zuo-Yan¹, SUN Yong-Hua², ZHAO Jue¹

1. College of Life Sciences, Peking University, Beijing 100871, China 2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Received:2012-06-18 Revised:2012-09-12 Online:2013-04-20 Published:2013-04-25

摘要/Abstract

摘要： 鱼类核移植是动物克隆研究的一个重要领域, 我国学者在上世纪60年代首创了鱼类的核移植研究。以斑马鱼为模式动物, 进行核移植与再程序化研究具有独特的优势。文章总结了鱼类细胞核移植研究的历史、斑马鱼核移植研究概况、以及影响核移植胚胎发育的因素, 特别是核移植胚胎基因组的表观遗传修饰, 如基因组DNA甲基化及组蛋白乙酰化和甲基化等的研究, 将有助于完善克隆技术并提高克隆的成功率, 推动克隆技术的广泛开展和应用。

关键词: 核移植, 斑马鱼, 再程序化, 表观遗传修饰

Abstract: As an important sub-field in the study of animal cloning, fish nuclear transfer was first established in the early 1960s by Chinese embryologists. Due to its advantages, zebrafish has become a unique animal model to study the mystery of reprogramming in nuclear transfer. This article summarizes the history and current situation in fish nuclear transfer tech-nology and discusses the factors that may influence the development of the cloned embryos. A comprehensive understand-ing of the mechanism for epigenetic modification following nuclear transfer, such as genomic DNA methylation and histone acetylation and/or methylation, will likely increase the success rate and eventually lead to the future freedom of cloning technique.

Key words: nuclear transfer, zebrafish, reprogramming, epigenetic modification

王学耕朱作言孙永华赵珏. 鱼类核移植与重编程[J]. 遗传, 2013, 35(4): 433-440.

WANG Xue-Geng ZHU Zuo-Yan SUN Yong-Hua ZHAO Jue. Nuclear transfer and reprogramming in fish[J]. HEREDITAS, 2013, 35(4): 433-440.

参考文献

[1] Spemann H. Embryonic development and induction. New Haven: Yale University Press, 1938, 210-211.
[2] Briggs R, King TJ. Transplantation of living nuclei from blastula cells into enucleated frogs’ eggs. Proc Natl Acad Sci USA, 1952, 38(5): 455-463.
[3] 童第周, 吳尚懃,叶毓芬, 嚴絙顄, 杜淼, 陸德裕. 鱼类细胞核的移植. 科学通报, 1963, (7): 60-61.
[4] Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mam-malian cells. Nature, 1997, 385(6619): 810-813.
[5] 童第周, 叶毓芬, 陆德裕, 童夙明, 杜淼. 鱼类不同亚科间的细胞核移植. 动物学报, 1973, 19(3): 201-212.
[6] 吴尚懃, 蔡难儿, 徐权汉. 不同品系金鱼间细胞核的多代移植. 实验生物学报, 1980, 13(1): 65-77.
[7] 中国科学院动物研究所细胞遗传研究组, 中国科学院水生生物研究所体细胞遗传研究组, 水产总局长江水产研究所细胞核移植研究组. 硬骨鱼类的细胞核移植——鲤鱼细胞核和鲫鱼细胞质配合的杂种鱼. 中国科学, 1980, (4): 376-380.
[8] Sun YH, Chen SP, Wang YP, Hu W, Zhu ZY. Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and gold-fish (Carassius auratus) enucleated eggs. Biol Reprod, 2005, 72(3): 510-515.
[9] 严绍颐, 陆德裕, 杜淼, 李光三, 林礼堂, 靳光琴, 王洪, 杨永铨, 夏德全, 刘爱珠, 朱作言, 易詠兰, 陈敏容, 杨兴棋, 陈宏溪. 硬骨鱼类的细胞核移植——鲫鱼细胞核和鲤鱼细胞质配合的杂种鱼. 中国科学(B辑), 1984, (8): 729-731.
[10] 陈宏溪, 易詠兰, 陈敏容, 杨兴棋. 鱼类培养细胞核发育潜能的研究. 水生生物学报, 1986, 10(1): 1-7.
[11] 张念慈, 曹铮, 尹文林, 吴乐丁, 沈锦玉, 毛树坚, 张铭, 杭绮, 赵小立. 草鱼、青鱼体外培养细胞的属间、亚科间核移植. 水产学报, 1990, 14(4): 344-346.
[12] Lee KY, Huang HG, Ju BS, Yang ZG, Lin S. Cloned ze-brafish by nuclear transfer from long-term-cultured cells. Nat Biotechnol, 2002, 20(8): 795-799.
[13] 李荔, 张士璀, 王锐, 李国荣, 王梅林. 斑马鱼的克隆: 囊胚细胞核移入正常具核卵子的发育. 高技术通讯, 2000, 10(7): 24-27.
[14] 李荔, 张士璀, 李红岩, 郭华荣. 斑马鱼体细胞核移植——头肾细胞和尾鳍细胞核移入成熟具核卵子发育能力的初步研究. 青岛海洋大学学报 (自然科学版), 2002, 32(1): 73-78.
[15] Hattori M, Hashimoto H, Bubenshchikova E, Wakamatsu Y. Nuclear transfer of embryonic cell nuclei to non-enucleated eggs in zebrafish, Danio rerio. Int J Biol Sci, 2011, 7(4): 460-468.
[16] 胡炜, 汪亚平, 陈尚萍, 朱作言. 不同品系间斑马鱼的细胞核移植. 科学通报, 2001, 46(24): 2062-2065.
[17] Meissner A, Jaenisch R. Mammalian nuclear transfer. Dev Dyn, 2006, 235(9): 2460-2469.
[18] Siripattarapravat K, Pinmee B, Venta PJ, Chang CC, Ci-belli JB. Somatic cell nuclear transfer in zebrafish. Nat Meth, 2009, 6(10): 733-735.
[19] Bhutani N, Brady JJ, Damian M, Sacco A, Corbel SY, Blau HM. Reprogramming towards pluripotency requires AID-dependent DNA demethylation. Nature, 2010, 463(7284): 1042-1047.
[20] Pei DS, Sun YH, Chen SP, Wang YP, Hu W, Zhu ZY. Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs. Theriogenology, 2007, 68(9): 1282-1291.
[21] Luo D, Hu W, Chen S, Xiao Y, Sun Y, Zhu Z. Identification of differentially expressed genes between cloned and zygote-developing zebrafish (Danio rerio) embryos at the dome stage using suppression subtractive hy-bridization. Biol Reprod, 2009, 80(4): 674-684.
[22] Siripattarapravat K, Pinmee B, Chang EA, Munoz JD, Kawakami K, Cibelli JB. The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer. Int J Dev Biol, 2010, 54(11-12): 1679-1683.
[23] Wells DN, Laible G, Tucker FC, Miller AL, Oliver JE, Xiang T, Forsyth JT, Berg MC, Cockrem K, L’Huillier PJ, Tervit HR, Oback B. Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle. Theriogenology, 2003, 59(1): 45-59.
[24] Kwon OY, Kono T. Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos. Proc Natl Acad Sci USA, 1996, 93(23): 13010-13013.
[25] Niwa K, Ladygina T, Kinoshita M, Ozato K, Wakamatsu Y. Transplantation of blastula nuclei to non-enucleated eggs in the medaka, Oryzias latipes. Dev Growth Differ, 1999, 41(2): 163-172.
[26] Lee EJ, Lee BB, Kim SJ, Park YD, Park

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

鱼类核移植与重编程

Nuclear transfer and reprogramming in fish

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	李凯伦, 卢荆奥, 陈小辉, 张文清, 刘伟. 尿囊素促进破骨细胞缺陷斑马鱼骨折修复[J]. 遗传, 2023, 45(4): 341-353.
[2]	卢荆澳, 黄春燕, 林芷茵, 唐政, 马宁, 黄志斌. cd99l2基因调控斑马鱼白细胞组织间的迁移机制[J]. 遗传, 2022, 44(9): 798-809.
[3]	宋绍征, 何正义, 成勇, 于宝利, 张婷, 李丹. TALENs介导MSTN基因突变山羊的制备及性能分析[J]. 遗传, 2022, 44(6): 531-542.
[4]	郑鹏飞, 谢海波, 朱盼盼, 赵呈天. 斑马鱼神经底板处神经元的分布及特征[J]. 遗传, 2022, 44(6): 510-520.
[5]	曲卉, 柳毅, 陈雅文, 汪晖. 环境因素所致印迹基因改变与子代器官发育[J]. 遗传, 2022, 44(2): 107-116.
[6]	张婷婷, 刘峰. 斑马鱼蛋白酪氨酸硫酸化修饰的检测方法研究[J]. 遗传, 2022, 44(2): 178-186.
[7]	张杨景晖, 常沛瑶, 杨紫淑, 薛宇航, 李雪奇, 张旸. 表观遗传修饰影响花青苷合成研究进展[J]. 遗传, 2022, 44(12): 1117-1127.
[8]	赵清雯, 潘东宁. 表观遗传修饰对脂肪组织产热的调控进展[J]. 遗传, 2022, 44(10): 867-880.
[9]	贾婷婷, 雷蕾, 吴歆媛, 蔡顺有, 陈艺璇, 薛钰. 二甲双胍对斑马鱼骨骼发育及损伤修复的机制研究[J]. 遗传, 2022, 44(1): 68-79.
[10]	王雅楠, 徐涛, 王万鹏, 张庆祝, 解莉楠. 表观遗传修饰在作物重要性状形成中的作用[J]. 遗传, 2021, 43(9): 858-879.
[11]	周俊, 赵成成, 吴霄, 石俊松, 周荣, 吴珍芳, 李紫聪. 猪耳成纤维细胞转录组异质性及对核移植胚胎发育的潜在影响[J]. 遗传, 2020, 42(9): 898-915.
[12]	郭佳妮, 刘帆, 王璐. 斑马鱼血液疾病模型及应用[J]. 遗传, 2020, 42(8): 725-738.
[13]	吴杰, 全建平, 叶勇, 吴珍芳, 杨杰, 杨明, 郑恩琴. 染色质转座酶可及性测序研究进展[J]. 遗传, 2020, 42(4): 333-346.
[14]	敖政, 陈祥, 吴珍芳, 李紫聪. 体细胞克隆猪发育异常研究进展[J]. 遗传, 2020, 42(10): 993-1003.
[15]	杨旭琼, 吴珍芳, 李紫聪. 哺乳动物体细胞核移植表观遗传重编程研究进展[J]. 遗传, 2019, 41(12): 1099-1109.