遗传 ›› 2013, Vol. 35 ›› Issue (4): 433-440.doi: 10.3724/SP.J.1005.2013.00433

• 综述 • 上一篇    下一篇

鱼类核移植与重编程

王学耕1, 朱作言1, 孙永华2, 赵珏1   

  1. 1. 北京大学生命科学学院, 北京 100871 2. 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉 430072
  • 收稿日期:2012-06-18 修回日期:2012-09-12 出版日期:2013-04-20 发布日期:2013-04-25
  • 通讯作者: 赵珏 E-mail:zhaojue@pku.edu.cn
  • 基金资助:

    斑马鱼细胞核移植发育再程序化研究项目(编号:30950001)资助

Nuclear transfer and reprogramming in fish

WANG Xue-Geng1, ZHU Zuo-Yan1, SUN Yong-Hua2, ZHAO Jue1   

  1. 1. College of Life Sciences, Peking University, Beijing 100871, China 2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  • Received:2012-06-18 Revised:2012-09-12 Online:2013-04-20 Published:2013-04-25

摘要: 鱼类核移植是动物克隆研究的一个重要领域, 我国学者在上世纪60年代首创了鱼类的核移植研究。以斑马鱼为模式动物, 进行核移植与再程序化研究具有独特的优势。文章总结了鱼类细胞核移植研究的历史、斑马鱼核移植研究概况、以及影响核移植胚胎发育的因素, 特别是核移植胚胎基因组的表观遗传修饰, 如基因组DNA甲基化及组蛋白乙酰化和甲基化等的研究, 将有助于完善克隆技术并提高克隆的成功率, 推动克隆技术的广泛开展和应用。

关键词: 核移植, 斑马鱼, 再程序化, 表观遗传修饰

Abstract: As an important sub-field in the study of animal cloning, fish nuclear transfer was first established in the early 1960s by Chinese embryologists. Due to its advantages, zebrafish has become a unique animal model to study the mystery of reprogramming in nuclear transfer. This article summarizes the history and current situation in fish nuclear transfer tech-nology and discusses the factors that may influence the development of the cloned embryos. A comprehensive understand-ing of the mechanism for epigenetic modification following nuclear transfer, such as genomic DNA methylation and histone acetylation and/or methylation, will likely increase the success rate and eventually lead to the future freedom of cloning technique.

Key words: nuclear transfer, zebrafish, reprogramming, epigenetic modification