遗传 ›› 1998, Vol. 20 ›› Issue (3): 19-22.
侯林1; 邹向阳2; 蔡含筠1 HOU Lin1;ZOU Xiang-yang2;CAI Han-jun1
摘要: 采用聚丙烯酰胺凝胶垂直板电泳法对中国两性生殖卤虫12个品系的苹果酸脱氢酶(MDH)同工酶基因的表达进行了研究。实验结果表明:(1)由于一些MDH同工酶基因所编码产生的亚基相互结合的频率较低,故没有得到理论上应得到的基因型数;(2)存在品系内和品系间的多态现象;(3)A. sini ca与A.parthenogenet ica之间存在高度的遗传变异和分化,另外,二者间具有相同的基因型和较高的遗传相似系数值也说明二者间存在一定的亲缘关系。
Abstract:Expression of malate dehydrogenase isozymic genes of 12 bisexual Artemia strains from China was strudied by means of polyacrylamide gel electrophorsis method.The experimental results showed are as follows,(1)Because of low frequency of some isozymic subunits combining each other and small number of experimental samples,we did not get the theoretical number of genotype that should appeare;(2)A.sinaca and A.parthenogenetica have characteristic MDH isozymic patterns,this provides precise biochemical index for distinguishing different Artemia strains;(3)MDH isozyme in 12 bisexual Artemia strainis is polymorphic.