遗传 ›› 2008, Vol. 30 ›› Issue (9): 1187-1194.doi: 10.3724/SP.J.1005.2008.01187

• 研究报告 • 上一篇    下一篇

中国部分家养山羊mtDNA D环区遗传多样性与进化

郝荣超1; 昝林森1; 刘丑生2; 王志刚2; 张桂香2; 韩旭2; 郝怀志1; 王静3; 杜小勇4

  

  1. 1. 西北农林科技大学动物科技学院, 杨凌 712100;
    2. 全国畜牧兽医总站畜禽牧草种质资源保存利用中心, 北京 100193;
    3. 甘肃农业大学动物科学技术学院, 兰州 730070;
    4. 华中农业大学动物科技学院, 武汉 430070

  • 收稿日期:2008-01-04 修回日期:2008-03-12 出版日期:2008-09-10 发布日期:2008-09-10
  • 通讯作者: 郝荣超

Genetic diversity and origin of mitochondrial DNA D-loop region for some domestic goat breeds of China

HAO Rong-Chao1; ZAN Lin-Sen1; LIU Chou-Sheng2; WANG Zhi-Gang2; ZHANG Gui-Xiang2; HAN Xu2; HAO Huai-Zhi1; WANG Jing3; DU Xiao-Yong4

  

  1. 1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;
    2. National Center for Preservation and Utilization of Genetic Resources of Domestic Animals and Forages, National Animal Husbandry & Veterinary Service, Beijing 100193, China;
    3. Animal science and technologyy Facult of Gansu Agricultural University, Lanzhou 730070, China;
    4. Animal science and technologyy Facult of Huazhong Agricultural University, Wuhan 430070, China
  • Received:2008-01-04 Revised:2008-03-12 Online:2008-09-10 Published:2008-09-10
  • Contact: HAO Rong-Chao

PDF (PC)

2224

被引次数

8

摘要：

对我国10个家养山羊品种140只个体的mtDNA D-loop区进行测序分析, 测得结果: 整个D-loop区为 1 211~1 213 bp, 共检测到84种单倍型, 171个多态位点。其中核苷酸多样性(Nucleotide diversity, Pi)为: 0.02063 ± 0.00225, 单倍型多样性(Haplotype gene diversity, Hd)为: 0.988 ± 0.003, 平均核苷酸差异数(Average number of nucleotide differences) k为: 24.896。表明我国家养山羊品种遗传多样性丰富。通过构建NJ网络进化树, 得出中国家养山羊主要分为两大支系, 并且其中一支与角骨羊(Capra aegagrus)聚在一起, 而旋角野山羊(Capra fal-coneri)单独聚为一支, 说明角骨羊对中国家养山羊贡献较大。

关键词: 山羊, mtDNA D-loop, 遗传多样性, 起源进化

Abstract:

The complete sequences of mitochondrial DNA D-loop of 140 individuals in 10 Chinese goat (Capra hiruc) breeds were analyzed by DNA sequencing technology. The results showed that the length of mtDNA D-loop in Chinese goats was 1 211-1 213 bp. There were 84 different haplotypes and 171 polymorphic sites. The mean nucleotide diversity (Pi) and haplotype diversity (Hd) were 0.02063 ± 0.00225 and 0.988 ± 0.003, respectively, and the average number of nucleotide differences (k) was 24.896. The results showed an abundant genetic diversity in domestic goats of China. The NJ tree indicated that there were two main branches in Chinese domestic goats, thereinto, one branch was clustered with Capra aegagrus, and Capra falconeri was clustered alone, which indicated that Capra aegagrus had more contribution to Chinese domestic goat breeds.

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