玉米幼胚胚性愈伤组织再生能力相关性状遗传研究

doi:10.16288/j.yczz.16-323

遗传 ›› 2017, Vol. 39 ›› Issue (2): 143-155.doi: 10.16288/j.yczz.16-323

玉米幼胚胚性愈伤组织再生能力相关性状遗传研究

张晓玲(),龙芸,葛飞,管中荣,张晓祥,王艳丽,沈亚欧(),潘光堂()

四川农业大学玉米研究所,成都 611130

收稿日期:2016-09-20 修回日期:2017-01-13 出版日期:2017-02-20 发布日期:2017-01-24
作者简介:张晓玲,硕士研究生,专业方向：作物分子育种原理与方法。E-mail: lindaisclever@foxmail.com|沈亚欧,博士,研究员,研究方向：玉米重要性状基因功能及转基因育种。E-mail: shenyaou@aliyun.com|潘光堂,博士,教授,研究方向：玉米数量性状遗传与玉米种质资源发掘创新与利用。E-mail: pangt@sicau.edu.cn
基金资助:
基金项目: 国家自然科学基金项目(31471512)

A genetic study of the regeneration capacity of embryonic callus from the maize immature embryo culture

Zhang Xiaoling(),Long Yun,Ge Fei,Guan Zhongrong,Zhang Xiaoxiang,Wang Yanli,Shen Yaou(),Pan Guangtang()

Maize Research Institute of Sichuan Agricultural University, Chengdu 611130, China

Received:2016-09-20 Revised:2017-01-13 Online:2017-02-20 Published:2017-01-24
Supported by:
the National Nature Science Foundation of China(31471512)

摘要/Abstract

摘要：

本文对来源于美国、墨西哥和中国的144份不同玉米自交系幼胚胚性愈伤组织的再生能力相关性状进行了研究,发现其再生能力受到环境、基因型及环境与基因型互作三方面的影响。其中各性状之间的相关性表现为：绿点率(green embryonic callus rate, GCR)、分化率(embryonic callus differentiating rate, CDR)及再生绿苗数(the plantlet number of embryonic callus regeneration, CPN)之间呈极显著正相关,且这三者与褐化率(embryonic callus browning rate, CBR)呈极显著负相关; 两次继代的克隆指数(embryonic callus cloning index for the first subculture, CCI1; embryonic callus cloning index for the second subculture, CCI2)呈显著正相关,且CCI2与GCR有一定的正相关关系,与CBR呈负相关关系;生根率(embryonic callus rooting rate, CRR)则与GCR、CDR及CPN呈一定正相关。经过广义遗传力计算发现：胚性愈伤组织的两次继代克隆指数CCI1、CCI2和CRR的遗传力较低,其他性状的遗传力较高。此外,经Ward法双向聚类分析,共发现了11个具有高再生能力的自交系材料,且通过生根培养发现其再生绿苗的生根情况良好,因而可将它们作为玉米转基因受体的骨干自交系。

关键词: 幼胚, 胚性愈伤组织, 再生能力, 环境, 基因型

Abstract:

We carried out a study of regeneration capacities of embryonic callus from maize immature embryo culture with 144 different inbred lines of natural groups from different countries, and found that the regeneration capacity was affected by three factors: environment, genotype and the interaction between the environment and genotype. We found that green embryonic callus rate (GCR), embryonic callus differentiating rate (CDR) and the plantlet number of embryonic callus regeneration (CPN) have significant positive correlations with each other, and they all have significant negative correlations with embryonic callus browning rate (CBR). Moreover, embryonic callus cloning index for the first subculture (CCI1) and embryonic callus cloning index for the second subculture (CCI2) have a significant positive correlation with each other, and CCI2 is positively correlated with green GCR, and is negatively correlated with CBR. Embryonic callus rooting rate (CRR) is positively correlated with GCR, CDR and CPN to some degree. Furthermore, we calculated Broad-Sense Heritability of each trait, and uncovered that the heritability index of CCI1, CCI2 and CRR was lower, and the heritability index of others was higher. In addition, by using the Ward method for two-way cluster analysis, we found eleven inbred lines with high regenerating abilities, and the rooting situation of regenerating plantlet was excellent by rooting culture, which could be used as the elite inbred lines of the maize transgenic receptor.

Key words: immature embryo, embryonic callus, regeneration capacity, environment, genotype

张晓玲,龙芸,葛飞,管中荣,张晓祥,王艳丽,沈亚欧,潘光堂. 玉米幼胚胚性愈伤组织再生能力相关性状遗传研究[J]. 遗传, 2017, 39(2): 143-155.

Zhang Xiaoling,Long Yun,Ge Fei,Guan Zhongrong,Zhang Xiaoxiang,Wang Yanli,Shen Yaou,Pan Guangtang. A genetic study of the regeneration capacity of embryonic callus from the maize immature embryo culture[J]. Hereditas(Beijing), 2017, 39(2): 143-155.

参考文献

[1]	Armstrong CL, Green CE. Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta, 1985, 164(2): 207-214.
[2]	Shen Y, Jiang Z, Yao XD, Zhang ZM, Lin HJ, Zhao MJ, Liu HL, Peng HW, Li SJ, Pan GT. Genome expression profile analysis of the immature maize embryo during dedifferentiation. PLoS One, 2012, 7(3): e32237.
[3]	Mu GQ, Pan GT, Liu YZ, Xia YL. Preliminary study on maize genotypes and the establishment of embryogenic callus. J Sichuan Agric Univ, 2003, 21(1): 13-17.
[3]	母贵琴, 潘光堂, 刘玉贞, 夏燕莉. 玉米基因型与幼胚培养胚性愈伤组织发生能力的初步研究. 四川农业大学学报, 2003, 21(1): 13-17.
[4]	Yuan Y, Liu DP, Zheng PH, Chen YB, Wen G. Influencing effect of induction of embryonic callus in maize inbred lines of northeast. J Maize Sci, 2001, 9(1): 37-38.
[4]	袁鹰, 刘德璞, 郑培和, 陈一斌, 温钢. 东北玉米自交系胚性愈伤组织的诱导. 玉米科学, 2001, 9(1): 37-38.
[5]	Ma L, Sun QQ, Zhou YL, Wu CL, Zhang CQ. Study on the relationship between inheritance and immature embryo culturing capacity of maize inbreds. J Mol Cell Biol, 2007, 40(2): 164-172.
[5]	马丽, 孙庆泉, 周玉亮, 吴承来, 张春庆. 玉米自交系幼胚培养能力与遗传关系研究(简报). 分子细胞生物学报, 2007, 40(2): 164-172.
[6]	Song Y, Xia YL, Wei X, Zhang ZM, Zhao MJ, Rong TZ, Pan GT. Analysis on gene effect of four characters of immature embryo culture in maize. Sci Agric Sin, 2006, 39(1): 18-22.
[6]	宋芸, 夏燕莉, 魏昕, 张志明, 赵茂俊, 荣廷昭, 潘光堂. 四种玉米幼胚培养性状的基因效应分析. 中国农业科学, 2006, 39(1): 18-22.
[7]	Li Z. Sequence diversity of ZmLEC1 and association analysis of embryogenic calli formation ability in maize[D]. Harbin: Northeast Agricultural University, 2013.
[7]	李钊. 玉米再生候选基因ZmLEC1的序列变异及其与胚性愈伤形成能力的关联分析[学位论文]. 哈尔滨: 东北农业大学, 2013.
[8]	Xie YJ, Dai JR, Hao K. Studies on induction of embrynic in maize and regeneration of its plantlet. Hereditas (Beijing), 1987, 9(3): 11-13.
[8]	谢友菊, 戴景瑞, 郝琨. 玉米胚性愈伤组织的诱导及其植株再生的研究. 遗传, 1987, 9(3): 11-13.
[9]	Prioli LM, Da Silva WJ, S?ndahl MR. Tissue, cell, and protoplast culture of maize (Zea mays L.). In: Nijkamp H J J, Van Der Plas L H W, Van Aartrijk J, eds. Progress in Plant Cellular and Molecular Biology. Netherlands: Springer, 1990: 38-43.
[10]	Tomes DT, Smith OS. The effect of parental genotype on initiation of embryogenic callus from elite maize (Zea mays L.) germplasm. Theor Appl Genet, 1985, 70(5): 505-509.
[11]	Armstrong CL, Green CE, Phillips RL, Stucker RE. Genetic control of plant regeneration from maize tissue cultures. Maize Genet Coop Newsl, 1985, 59: 92-93.
[12]	Sun SM, Xun LJ, He ZC, Jiang W. Analysis of embryonic callus frequency and genetic rule in maize. J Laiyang Agric Coll, 1995, 12(3): 173-176.
[12]	孙世孟, 徐丽娟, 何忠诚, 姜伟. 玉米胚性愈伤组织发生频率及其遗传规律的研究. 莱阳农学院学报, 1995, 12(3): 173-176.
[13]	Armstrong CL, Romero-severson J, Hodges TK. Improved tissue culture response of an elite maize inbred through backcross breeding, and identification of chromosomal regions important for regeneration by RFLP analysis. Theor Appl Genet, 1992, 84(5-6): 755-762.
[14]	Mu GQ. Embryonic callus screening and its cloning ability analysis of different genotype maize[D]. Ya’an: Sichuan Agricultural University, 2001.
[14]	母贵琴. 不同基因型玉米胚性愈伤组织的筛选及其克隆能力的研究[学位论文]. 雅安: 四川农业大学, 2001.
[15]	Song Y. Genetic analysis of main characters from immature embryo culture in maize[D]. Ya’an: Sichuan Agricultural University, 2004.
[15]	宋芸. 玉米幼胚培养主要性状的基因效应分析[学位论文]. 雅安: 四川农业大学, 2004.
[16]	Pan GT, Zhang ZM, Wei X, Song Y, Zhao MJ, Xia YL, Rong TZ. QTL analysis of maize (Zea mays L.) embryo culturing capacity. Acta Agro Sin, 2006, 32(1): 7-13.
[16]	潘光堂, 张志明, 魏昕, 宋芸, 赵茂俊, 夏燕莉, 荣廷昭. 玉米幼胚培养能力性状QTL分析. 作物学报, 2006, 32(1): 7-13.
[17]	Robinson GK. That BLUP is a good thing: the estimation of random effects. StatistSci, 1991, 6(1): 15-32.
[18]	Piepho HP, M?hring J, Melchinger AE, Büchse A. BLUP for phenotypic selection in plant breeding and variety testing. Euphytica, 2008, 161(1-2): 209-228.
[19]	Liu XQ, Rong JY, Liu XY. Best linear unbiased prediction for linear combinations in general mixed linear models. J Mult Anal, 2008, 99(8): 1503-1517.
[20]	Merk HL. Estimating heritability and BLUPs for traits using tomato phenotypic data. eXtension.org, 2014.

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玉米幼胚胚性愈伤组织再生能力相关性状遗传研究

A genetic study of the regeneration capacity of embryonic callus from the maize immature embryo culture

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