遗传 ›› 2017, Vol. 39 ›› Issue (2): 143-155.doi: 10.16288/j.yczz.16-323

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玉米幼胚胚性愈伤组织再生能力相关性状遗传研究

张晓玲(),龙芸,葛飞,管中荣,张晓祥,王艳丽,沈亚欧(),潘光堂()   

  1. 四川农业大学玉米研究所,成都 611130
  • 收稿日期:2016-09-20 修回日期:2017-01-13 出版日期:2017-02-20 发布日期:2017-01-24
  • 作者简介:张晓玲,硕士研究生,专业方向：作物分子育种原理与方法。E-mail: lindaisclever@foxmail.com|沈亚欧,博士,研究员,研究方向：玉米重要性状基因功能及转基因育种。E-mail: shenyaou@aliyun.com|潘光堂,博士,教授,研究方向：玉米数量性状遗传与玉米种质资源发掘创新与利用。E-mail: pangt@sicau.edu.cn
  • 基金资助:
    基金项目: 国家自然科学基金项目(31471512)

A genetic study of the regeneration capacity of embryonic callus from the maize immature embryo culture

Xiaoling Zhang(),Yun Long,Fei Ge,Zhongrong Guan,Xiaoxiang Zhang,Yanli Wang,Yaou Shen(),Guangtang Pan()   

  1. Maize Research Institute of Sichuan Agricultural University, Chengdu 611130, China
  • Received:2016-09-20 Revised:2017-01-13 Online:2017-02-20 Published:2017-01-24
  • Supported by:
    the National Nature Science Foundation of China(31471512)

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摘要：

本文对来源于美国、墨西哥和中国的144份不同玉米自交系幼胚胚性愈伤组织的再生能力相关性状进行了研究,发现其再生能力受到环境、基因型及环境与基因型互作三方面的影响。其中各性状之间的相关性表现为：绿点率(green embryonic callus rate, GCR)、分化率(embryonic callus differentiating rate, CDR)及再生绿苗数(the plantlet number of embryonic callus regeneration, CPN)之间呈极显著正相关,且这三者与褐化率(embryonic callus browning rate, CBR)呈极显著负相关; 两次继代的克隆指数(embryonic callus cloning index for the first subculture, CCI1; embryonic callus cloning index for the second subculture, CCI2)呈显著正相关,且CCI2与GCR有一定的正相关关系,与CBR呈负相关关系;生根率(embryonic callus rooting rate, CRR)则与GCR、CDR及CPN呈一定正相关。经过广义遗传力计算发现：胚性愈伤组织的两次继代克隆指数CCI1、CCI2和CRR的遗传力较低,其他性状的遗传力较高。此外,经Ward法双向聚类分析,共发现了11个具有高再生能力的自交系材料,且通过生根培养发现其再生绿苗的生根情况良好,因而可将它们作为玉米转基因受体的骨干自交系。

关键词: 幼胚, 胚性愈伤组织, 再生能力, 环境, 基因型

Abstract:

We carried out a study of regeneration capacities of embryonic callus from maize immature embryo culture with 144 different inbred lines of natural groups from different countries, and found that the regeneration capacity was affected by three factors: environment, genotype and the interaction between the environment and genotype. We found that green embryonic callus rate (GCR), embryonic callus differentiating rate (CDR) and the plantlet number of embryonic callus regeneration (CPN) have significant positive correlations with each other, and they all have significant negative correlations with embryonic callus browning rate (CBR). Moreover, embryonic callus cloning index for the first subculture (CCI1) and embryonic callus cloning index for the second subculture (CCI2) have a significant positive correlation with each other, and CCI2 is positively correlated with green GCR, and is negatively correlated with CBR. Embryonic callus rooting rate (CRR) is positively correlated with GCR, CDR and CPN to some degree. Furthermore, we calculated Broad-Sense Heritability of each trait, and uncovered that the heritability index of CCI1, CCI2 and CRR was lower, and the heritability index of others was higher. In addition, by using the Ward method for two-way cluster analysis, we found eleven inbred lines with high regenerating abilities, and the rooting situation of regenerating plantlet was excellent by rooting culture, which could be used as the elite inbred lines of the maize transgenic receptor.

Key words: immature embryo, embryonic callus, regeneration capacity, environment, genotype