遗传 ›› 2012, Vol. 34 ›› Issue (4): 383-388.doi: 10.3724/SP.J.1005.2012.00383

• 综述 •    下一篇

miRNA*生物合成及其功能研究的新发现

马圣运, 白玉, 韩凝, 王君晖, 翁晓燕, 边红武, 朱睦元   

  1. 浙江大学生命科学院遗传所, 杭州 310058
  • 收稿日期:2011-11-11 修回日期:2012-01-05 出版日期:2012-04-20 发布日期:2012-04-25
  • 通讯作者: 边红武 E-mail:hwbian@zju.edu.cn
  • 基金资助:

    国家自然科学基金项目(编号:30972016, 31171615, 31171543)和浙江省自然科学基金(编号:Y3090247)资助

Recent research progress of biogenesis and functions of miRNA

MA Sheng-Yun, BAI Yu, HAN Ning, WANG Jun-Hui, WENG Xiao-Yan, BIAN Hong-Wu, ZHU Mu-Yuan   

  1. Institution of Genetics, College of Life Science, Zhejiang University, Hangzhou 310058, China
  • Received:2011-11-11 Revised:2012-01-05 Online:2012-04-20 Published:2012-04-25

摘要: miRNA*是在miRNA加工成熟过程中与其互补的大约22个核苷酸的RNA序列。传统观点认为miRNA*是miRNA生物合成中形成的没有功能的副产品。然而最近的研究发现miRNA*s与miRNA一样, 主要介导转录后的基因调控网络; 但不同的是, miRNA与Argonaute1蛋白(AGO1)结合形成RNA诱导的沉默复合体(RISC), 而miRNA*却在AGO2的帮助下形成RISC复合体进行RNA干涉, 这点与siRNA的作用方式类似。文章从miRNA*的生物合成、生物学特性和功能等方面综述了miRNA*最新研究进展。

关键词: miRNA*, miRNA, RNA干涉, AGO, miR/miR*复合体

Abstract: MicroRNA*s are about 22nt noncoding RNAs, which are processed from precursors with a characteristic hairpin secondary structure in the biogenesis of microRNAs. Recently, miRNA* strands were shown to mediate post-transcriptional regulatory networks, rather than serve merely as non-functional by-product in general view. Unlike miRNAs bound to AGO1, miRNA* strands are bound to AGO2 to form RISC duplex to mediate RNAi, which is similar to siRNA. This paper mainly reviewed the recent research progresses on miRNA*, such as the biosynthesis, biological characteristics, and functions.

Key words: miRNA*, miRNA, RNAi, AGO, miR/miR* duplex