遗传 ›› 2012, Vol. 34 ›› Issue (3): 342-347.doi: 10.3724/SP.J.1005.2012.00342

• 研究报告 • 上一篇    下一篇

TSA和VPA处理对食蟹猴-猪异种体细胞核移植胚胎早期发育的影响

秦祖兴1, 黄高波2, 罗军2, 宁淑芳1, 卢晟盛1, 卢克焕1   

  1. 1. 亚热带农业生物资源保护与利用国家重点实验室/广西大学动物繁殖研究所, 南宁 530004 2. 广西大学生命科学与技术学院, 南宁 530004
  • 收稿日期:2011-07-04 修回日期:2011-10-14 出版日期:2012-03-20 发布日期:2012-03-25
  • 通讯作者: 卢晟盛 E-mail:sslu@gxu.edu.cn
  • 基金资助:

    国家自然科学基金项目(批准号:30860039)和广西自然科学基金重点项目(编号:2010GXNSFD013021)资助

Effect of TSA and VPA treatment on long-tailed macaque (Macaca fascicularis)-pig interspecies somatic cell nuclear transfer

QIN Zu-Xing1, HUANG Gao-Bo2, LUO Jun2, NING Shu-Fang1, LU Sheng-Sheng1, LU Ke-Huan1   

  1. 1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Animal Reproduction Institute, Guangxi University, Nanning 530004, China 2. College of Life Science and Technology, Guangxi University, Nanning 530004, China
  • Received:2011-07-04 Revised:2011-10-14 Online:2012-03-20 Published:2012-03-25

摘要: 食蟹猴-猪异种体细胞核移植(Interspecies somatic cell nuclear transfer, iSCNT)研究旨在由iSCNT胚胎建立具有与人类相似遗传背景的胚胎干细胞 (ESCs), 作为医学和基础科学研究的实验材料。文章探讨了两种组蛋白脱乙酰化酶抑制剂(HDACi)-Trichostatin A(TSA)和Valproic acid(VPA)处理浓度、时间与培养液(PZM-3和HECM-10)组合对食蟹猴-猪iSCNT胚胎早期发育的影响。结果表明, 在PZM-3中添加10 nmol/L TSA处理48 h组的囊胚率显著高于对照组(22.78% vs 9.86%, P<0.05)。但是, 不管在PZM-3或是HECM-10中, 添加2~10 mmol/L VPA处理均不能提高iSCNT胚胎早期发育能力。文章证明了TSA处理可以提高食蟹猴-猪iSCNT胚胎早期发育能力。

关键词: 组蛋白脱乙酰化酶抑制剂(HDACi), 食蟹猴, 猪, 异种核移植, 胚胎发育

Abstract: Long-tailed macaque-pig interspecies somatic cell nuclear transfer (iSCNT) is beneficial to yield embryonic stem cells from iSCNT embryos with similar genetic background as human, which can be used as materials for medical and basic research. The primary objective of this study was to investigate the effects of concentrations and treatment duration of two histone deacetylase inhibitors–Trichostatin A (TSA) and Valproic acid (VPA) and two different embryo culture media (PZM-3 and HECM-10) on the in vitro development of iSCNT embryos. The results suggested that when PZM-3 was used as the embryo culture medium, the blastocyst rate of 10 nmol/L TSA treatment for 48 h was significantly higher than the control group (22.78% vs 9.86%, P<0.05). However, neither in PZM-3 nor in HECM-10, 2-10 mmol/L VPA treatment did not increase the in vitro developmental potential of iSCNT embryos. It was concluded that TSA treatment could enhance the in vitro developmental potential of long-tailed macaque-pig iSCNT embryos.

Key words: histone deacetylase inhibitors (HADCi), long-tailed macaque(Macaca fascicularis), pig, iSCNT, embryo development