一个水稻内颖退化突变体的形态特征及基因的精确定位

doi:10.3724/SP.J.1005.2012.01064

遗传 ›› 2012, Vol. 34 ›› Issue (8): 1064-1072.doi: 10.3724/SP.J.1005.2012.01064

一个水稻内颖退化突变体的形态特征及基因的精确定位

杨德卫, 卢礼斌, 程朝平, 曾美娟, 郑向华, 叶宁, 刘成德, 叶新福

福建省农业科学院水稻研究所, 福州 350018

收稿日期:2012-05-11 修回日期:2012-05-20 出版日期:2012-08-20 发布日期:2012-08-25
通讯作者: 叶新福 E-mail:yexinfu@126.com
基金资助:
福建省自然科学基金项目(编号：2011J01110), 福建省农业科学院青年人才创新基金项目(编号：2010QJ-A4), 福建省农业科学院科技创新团队建设重点科研项目(编号：CXTD2011-12)资助

Morphological characteristics and gene mapping of a palea degradation(pd2) mutant in rice

YANG De-Wei, LU Li-Bin, CHEN Chao-Ping, ZENG Mei-Juan, ZHENG Xiang-Hua, YE Ning, LIU Cheng-De, YE Xin-Fu

Institute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China

Received:2012-05-11 Revised:2012-05-20 Online:2012-08-20 Published:2012-08-25

摘要/Abstract

摘要： 水稻产量和品质受花器官发育的直接影响, 因此对水稻颖花发育机理的研究将有助于水稻产量提高和品质的改良。文章利用⁶⁰Coγ射线辐照亲本8PW33 (籼稻背景) 获得一个性状能稳定遗传的内颖退化突变体(编号：MU102), 并对其农艺性状和花器官进行了观察和分析。结果显示, 相对于野生型, 该突变体的株高、每穗总粒数及剑叶宽均显著增加, 而结实率则显著降低, 差异均达显著水平。解剖镜下观察表明, 该突变体内颖退化, 外颖弯曲呈现镰刀状, 其余器官与野生型表型基本一致。扫描电镜观察显示, 突变体与野生型叶片维管束的结构组成以及外颖表皮细胞组成、排列均正常, 没有明显差异; 与野生型相比, 突变体内颖表皮细胞排列较为紧密, 推测可能是内颖收缩退化导致的。遗传分析显示该突变性状是由隐性单基因控制, 并命名为pd2。利用实验室现有的SSR分子标记将PD2基因定位于水稻第9号染色体上, 通过进一步扩大群体和开发新的Indel标记, 将PD2基因定位在2个Indel标记之间, 两者间的物理距离大约是82 kb。在该物理区间内有一个已经克隆的内颖发育基因REP1, 经过测序和比对分析, 推测REP1与PD2为等位基因。

关键词: 水稻, 内颖退化, 突变体, 形态特征, 精细定位

Abstract: The yield and quality of rice are directly impacted by floral organ development in rice. Understanding of the floral development mechanism will be useful in genetic improvement of yield and quality. In this study, a rice mutant palea degradation 2 (pd2) in an indica cultivar ‘8PW33’ was obtained after ⁶⁰Co γ-ray treatment. Analysis of the mutant showed that, compared to the wild type, plant height, total grain number per panicle, and sword leaf width were significantly increased, but the seed setting rate were significantly decreased. The florets of the mutant exhibited degraded palea and sickle-shaped tortuous lemma. Detail examination using scanning electron microscopy revealed that when epidermis of the vane and lemma were normal, epidermis of the palea were arranged tightly, which might result from degraded palea. Genetic analysis supported that this mutation phenotype was controlled by a single recessive gene. Polymorphic analysis of simple sequence repeat markers demonstrated that PD2 gene is located on chromosome 9. With a larger mapping population and more indel markers, we further mapped PD2 gene between 2 indel markers with a physical region of about 82 kb. Within this region, there is a cloned gene REP1 known to control rice palea development. By comparing the DNA sequences of REP1 from pd2 and 8PW33, in combination with the results of phenotypic analysis, we concluded that PD2 is an allele of REP1.

Key words: rice, palea degradation, mutant, morphological characteristics, fine mapping

杨德卫，卢礼斌，程朝平，曾美娟，郑向华，叶宁，刘成德，叶新福. 一个水稻内颖退化突变体的形态特征及基因的精确定位[J]. 遗传, 2012, 34(8): 1064-1072.

YANG De-Wei, LU Li-Bin, CHEN Chao-Ping, ZENG Mei-Juan, ZHENG Xiang-Hua, YE Ning, LIU Cheng-De, YE Xin-Fu. Morphological characteristics and gene mapping of a palea degradation(pd2) mutant in rice[J]. HEREDITAS, 2012, 34(8): 1064-1072.

参考文献

[1] 陈少游, 王峰. 一个水稻生殖发育突变体的遗传分析及基因定位[学位论文]. 福州: 福建农林大学, 2008.
[2] 罗琼, 周开达, 刘国庆, 徐吉臣, 肖晗, 朱立煌. 水稻无内稃突变体的遗传分析和基因定位. 遗传学报, 2002, 29(31): 230-234.
[3] Zhang QF, Xu JD, Li Y, Xu PZ, Zhang HY, Wu XJ. Morphological, anatomical and genetic analysis for a rice mutant with abnormal hull. J Genet Genomics, 2007, 34(6): 519-526.
[4] 李云峰, 杨正林, 凌英华, 王楠, 任德勇, 王增, 何光华. 水稻多小花小穗突变体mf1的鉴定与基因定位. 作物学报, 2011, 37(2): 280-285.
[5] Coen ES, Meyerowitz EM. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353(6339): 31-37.
[6] Angenent GC, Franken J, Busscher M, van Dijken A, van Went JL, Dons HJ, van Tunen AJ. A novel class of MADS box genes is involved in ovule develop-ment in petunia. Plant Cell, 1995, 7(10): 1569-1582.
[7] Theissen G, Saedler H. Plant biology: floral quartets. Nature, 2001, 409(6819): 469 -471.
[8] Ferrario S, Immink RG, Shchennikova A, Busscher-Lange J, Angenent GC. The MADS box gene FBP2 is required for SEPALLATA function in petunia. Plant Cell, 2003, 15(4): 914- 925.
[9] Yamaguchi T, Lee DY, Miyao A, Hirochika H, An G, Hi-rano HY. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa. Plant Cell, 2006, 18(1): 15-28.
[10] Li Y, Xu PZ, Zhang HY, Peng H, Zhang QF, Wang XD, Wu XJ. Characterization and identification of a novel mu-tant fon(t) on floral organ number and flo-ral organ identity in rice. J Genet Genomics, 2007, 34(8): 730-737.
[11] 杨德卫, 叶新福. 水稻颖花突变体的最新研究进展. 分子植物育种, 2010, 8(1): 106-116.
[12] 杨德卫, 曾美娟, 卢礼斌, 叶宁, 刘成德, 郑向华, 叶新福. 一个水稻矮秆突变体的遗传分析及基因定位. 植物学报, 2011, 45(6): 617-624.
[13] Quarrie S, Lazic-Jancic V, Kovacevic D, Steed A, Pekic S. Bulk segregant analysis with molecular markers and its use for improving drought resistance in maize. J Exp Biol, 1999, 50(337): 1299-1306.
[14] Yuan Z, Gao S, Xue DW, Luo D, Li LT, Ding SY, Yao X, Wilson ZA, Qian Q, Zhang DB. RETARDED PALEA1 controls palea development and floral zy-gomorphy in rice. Plant Physiol, 2009, 149(1): 235-244.
[15] Ambrose BA, Lrner DR, Ciceri P, Padilla CM, Yanofsky MF, Schmidt RG. Molecular and genetics analyses of Sil-kyl gene reveal conservation in floral organ specification between eudicots and monocots. Mol Cell, 2000, 5(3): 569-579.
[16] 彭慧娟, 刘国华. 水稻无内稃突变体的花器形态观察与基因定位[学位论文]. 长沙: 湖南农业大学, 2007.
[17] Bossinger G, Rohde W, Lundquist U, Salamini F. Genetics of barley development: mutant phenotypes and molecular aspects. In barley: Genetics, Biochemistry, Molecular Biotechnology. Edited by Shewry PR, 1992, 231-263. CAB International, Wallingford.
[18] Zhu QH, Ramm K, Shivakkumar R, Dennis ES, Upadhyaya NM. The ANTHER INDEHISCENCE1 gene en-coding a single MYB domain protein is involved in anther development in rice. Plant Physiol, 2004, 135(3): 1514-1525.
[19] Park JJ, Jin P, Yoon J, Yang JI, Jeong H, Ranathunge K, Schreiber L, Franke R, Lee IJ, An G. Mutation in WILTED DWARF and LETHAl 1 (WDL1) causes abnormal cuticle formation and rapid water loss in rice. Plant Mol Biol, 2010, 74(1-2): 91-103.
[20] Suzaki T, Sato M, Ashikari M, Miyoshi M, Nagato Y, Hirano HY. The gene FLORAL ORGAN NUMBER1 regu-lates floral meristem size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1. Development, 2004, 131(22): 5649- 5657.
[21] Kalika P, Sriram P, Usha VH. OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs. Plant J, 2005, 43(6): 915-928.
[22] Lee SY, Kim J, Han JJ, Han MJ, An G. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog in rice. Plant J, 2004, 38(5): 754-764.
[23] Nonomura KI, Miyoshi K, Eiguchi M, Suzuki T, Miyao A, Hirochika H, Kurata N. The MSP1 gene is neces-sary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice. Plant Cell, 2003, 15(8): 1728- 1739.
[24] Luo H, Lee JY, Hu Q, Nelson-Vasilchik K, Eitas TK, Lickwar C, Kausch AP, Chandlee JM, Hodges TK. RTS, a rice anther-specific gene is required for male fertility and its promoter sequence directs tis-sue-specific gene expression in different plant species. Plant Mol Biol, 2003, 62(3): 397-408.
[25] Lee DY, Lee J, Moon S, Park SY, An G. The rice hetero-chronic gene SUPERNUMERARY BRACT regulates the transition from spikelet meristem to floral meris-tem. Plant J, 2006, 49(1): 64-78.
[26] Li N, Zhang DD, Liu HS, Yin CH, Li XX, Liang WQ, Yuan Z, Xu B, Chu HW, Wang J, Wen TQ, Huang H, Luo D, Ma H, Zhang DB. The rice TAPETUM DEGENERATION RETARDATION gene is required for tapetum degradation and anther development. Plant Cell, 2006, 18(11): 2999-3014.
[27] Jung KH, Han MJ, Lee YS, Kim YW, Hwang I, Kim MJ, Kim YK, Nahm BH, An G. Rice UNDEVELOPED TAPETUM1 is a major regulator of early tapetum de-velopment. Plant Cell, 2005, 17(10): 2705-2722.
[28] Jang S, Hur J, Kim SJ, Han MJ, Kim SR, An G. Ectopic expression of OsYAB1 causes extra stamens and carpels in rice. Plant Mol Biol, 2004, 56(1): 133-143.
[29] Li L, Yuan H, Barrena GV, Yang CY, Liang WQ, Zong J, Wilson ZA, Zhang DB. PERSISTENT TAPETAL CELL1 encodes a PHD-finger protein that is required for tapetal cell death and pollen development in rice. Plant Physiol, 2011, 156(2): 615-630.
[30] Yoshida A, Suzaki T, Tanaka W, Hirano HY. The homeotic gene long sterile lemma (G1) specifies sterile lemma identity in the rice spikelet. Proc Natl Acad Sci USA, 2009, 106 (47): 20103-20108.
[31] Li HF, Liang WQ, Jia RD, Yin CS, Zong J, Kong HZ, Zhang DB. The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice. Cell Res, 2010, 20 (3): 299-313.
[32] Hu LF, Liang WQ, Yin CS, Cui X, Zong J, Wang X, Hu JP, Zhang DB. Rice MADS3 regulates ROS homeostasis during late anther development. Plant Cell, 2011, 23(2): 515- 533.
[33] Li HF, Liang WQ, Yin CS, Zhu L, Zhang DB. Genetic interaction of OsMADS3, DROOPING LEAF, and OsMADS13 in specifying rice floral organ identities and meristem determinacy. Plant Physiol, 2011, 156(1): 263-274.
[34] Li HF, Liang WQ, Hua Y, Zhu L, Yin CS, Xu J, Dreni L, Kater MM, Zhang DB. Rice MADS6 interacts with the floral homeotic genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in specifying floral organ identities and meristem fate. Plant Cell, 2011, 23(7): 2536-2552.
[35] Jin Y, Luo Q, Tong HN, Wang AJ, Cheng ZJ, Tang JF, Li DY, Zhao XF, Li XB, Wan JM, Jiao YL, Chu CC, Zhu LH. An AT-hook gene is required for palea formation and flo-ral organ number control in rice. Dev Biol, 2011, 359(2): 277-288.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

一个水稻内颖退化突变体的形态特征及基因的精确定位

Morphological characteristics and gene mapping of a palea degradation(pd2) mutant in rice

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张桂权. 基于SSSL文库的水稻设计育种平台[J]. 遗传, 2019, 41(8): 754-760.
[2]	刘次桃, 王威, 毛毕刚, 储成才. 水稻耐低温逆境研究：分子生理机制及育种展望[J]. 遗传, 2018, 40(3): 171-185.
[3]	杨德卫, 郑向华, 程朝平, 叶宁, 黄凤凰, 叶新福. 基于CSSLs群体定位和图位克隆水稻长芒基因GAD1-2[J]. 遗传, 2018, 40(12): 1101-1111.
[4]	辛高伟, 胡熙璕, 王克剑, 王兴春. Cas9蛋白变体VQR高效识别水稻NGAC前间区序列邻近基序[J]. 遗传, 2018, 40(12): 1112-1119.
[5]	吴比, 胡伟, 邢永忠. 中国水稻遗传育种历程与展望[J]. 遗传, 2018, 40(10): 841-857.
[6]	李佳,刘运华,张余,陈晨,余霞,余舜武. 干旱对水稻生物钟基因和干旱胁迫响应基因每日节律性变化的影响[J]. 遗传, 2017, 39(9): 837-846.
[7]	韩晓斌, 徐冉, 段朋根, 于海跃, 罗越华, 李云海. 水稻斑点叶突变体spl101和spl102的筛选及候选基因鉴定[J]. 遗传, 2017, 39(4): 346-353.
[8]	袁金红, 李俊华, 袁娇娇, 贾克利, 李书粉, 邓传良, 高武军. 基于全基因组测序的MutMap方法在正向遗传学研究中的应用[J]. 遗传, 2017, 39(12): 1168-1177.
[9]	唐丽, 李曜魁, 张丹, 毛毕刚, 吕启明, 胡远艺, 韶也, 彭彦, 赵炳然, 夏石头. 基于基因组编辑技术的水稻靶向突变特征及遗传分析[J]. 遗传, 2016, 38(8): 746-755.
[10]	武迪, 黄林周, 高谨, 王永红. 植物重力反应的分子调控机制[J]. 遗传, 2016, 38(7): 589-602.
[11]	孔德艳, 陈守俊, 周立国, 高欢, 罗利军, 刘灶长. 水稻开花光周期调控相关基因研究进展[J]. 遗传, 2016, 38(6): 532-542.
[12]	李莉云,史佳楠,杨烁,孙财强,刘国振. 基于转录特征的水稻WRKY转录因子功能注释[J]. 遗传, 2016, 38(2): 126-136.
[13]	张红宇, 崔晓云, 侯飞雪, 王一伊, 吴挺开, 刘禹彤, 杨定乾, 张洪凯, 傅瑶, 张向阳, 李文丽, 吴先军. 水稻基因组加倍对籽粒大小调控基因表达的影响[J]. 遗传, 2016, 38(12): 1102-1111.
[14]	宋海冰, 汪斌, 陈壬杰, 郑小雅, 于世波, 兰涛. 水稻“光身”突变体glr3的遗传分析及基因定位[J]. 遗传, 2016, 38(11): 1012-1019.
[15]	胡运高, 郭连安, 杨国涛, 钦鹏, 范存留, 彭友林, 严维, 何航, 李仕贵. 直立密穗基因DEP2-1388的遗传分析及在杂交稻中的育种利用[J]. 遗传, 2016, 38(1): 72-81.