遗传 ›› 2002, Vol. 24 ›› Issue (2): 203-206.
黄骥1;张红生1;王东1;曹雅君1;杨金水2 HUANG Ji1;ZHANG Hong-sheng1;WANG Dong1;CAO Ya-jun1;YANG Jin-shui2
摘要: 基因表达系列分析(serial analysis of gene expression,SAGE)是近年来发展的以测序为基础,全面了解特定组织或细胞类型中基因群体表达状态的一项技术,它的显著特点是能够大量获取基因组范围基因表达的类别与丰度。SAGE技术已成功地应用于特异组织或细胞的转录组研究和mRNA群体间差异表达基因的鉴定。本文主要回顾了SAGE技术的原理、发展和应用。
Abstract:Serial analysis of gene expression (SAGE) is recently developed,a sequenced-based technique,which permits comprehensive and quantitative gene expression profiles from specific tissues or cells.SAGE has been successfully applied for transcriptome research and identification of differentially expressed genes between mRNA populations.This article mainly reviews the principle,development and application of SAGE.