基于转录组学分析秀丽线虫对乙酸钙不动杆菌的摄食偏好行为

doi:10.16288/j.yczz.24-364

遗传 ›› 2025, Vol. 47 ›› Issue (7): 786-796.doi: 10.16288/j.yczz.24-364

基于转录组学分析秀丽线虫对乙酸钙不动杆菌的摄食偏好行为

刘姣姣¹^,²(), 卢心河¹^,²(), 殷红彦³, 周海龙²(), 李汉增¹(), 徐顺清¹()

1.海南大学环境科学与工程学院，海口 570228
2.海南大学生命健康学院，海口 570228
3.海南大学热带农林学院，海口 570228

收稿日期:2024-12-02 修回日期:2025-01-15 出版日期:2025-07-20 发布日期:2025-03-13
通讯作者: 周海龙，博士，教授，研究方向：海洋环境生物学。E-mail: hlongzhou@163.com;
李汉增，博士，教授，研究方向：秀丽线虫遗传。E-mail: hanzeng.li@hainanu.edu.cn;
徐顺清，博士，教授，研究方向：环境污染物人群健康风险识别和评估。E-mail: Xus@hainanu.edu.cn
作者简介:刘姣姣，硕士研究生，专业方向：生物与医药(生物信息学)。E-mail: jjiaoliu@hainanu.edu.cn
卢心河，硕士研究生，专业方向：生物学(生物信息学)。E-mail: 22210710000043@hainanu.edu.cn
第一联系人：
刘姣姣和卢心河并列第一作者。
基金资助:
海南省重点研发项目(ZDYF2022SHFZ321);海南省自然科学基金项目(823QN230)

Analysis of food preference behavior of C. elegans to Acinetobacter calcoaceticus based on transcriptomics

Jiaojiao Liu¹^,²(), Xinhe Lu¹^,²(), Hongyan Yin³, Hailong Zhou²(), Hanzeng Li¹(), Shunqing Xu¹()

1. School of Environmental Science and Engineering, Hainan University, Haikou 570228, China
2. School of Life Health, Hainan University, Haikou 570228, China
3. School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China

Received:2024-12-02 Revised:2025-01-15 Published:2025-07-20 Online:2025-03-13
Supported by:
Hainan Province Key Research and Development Project(ZDYF2022SHFZ321);Natural Science Foundation of Hainan Province(823QN230)

摘要/Abstract

摘要：

秀丽线虫(C. elegans)是生物学研究的重要模式生物之一，在自然环境中选择细菌食物来源时表现出明显的偏好，然而食物选择这一与进化优势息息相关的生理现象背后的机制并不完全清楚。乙酸钙不动杆菌(Acinetobacter calcoaceticus，Ac)是一种广泛分布于潮湿环境中的革兰氏阴性杆菌。本研究通过比较秀丽线虫在两种细菌食源下的食物偏好性、咽泵频率、体脂含量、寿命长短以及基因表达差异，揭示了秀丽线虫摄食Ac引起脂质代谢变化以及摄食偏好行为，并利用转录组学方法分析了秀丽线虫摄食大肠杆菌OP50和Ac后的基因表达差异，为理解秀丽线虫与微生物互作机制提供了新的视角。

关键词: 秀丽线虫, 乙酸钙不动杆菌, 食物偏好, 转录组

Abstract:

C. elegans is one of the most important model organisms in biological research, exhibiting distinct preferences for bacterial food sources in natural environments. Acinetobacter calcoaceticus(Ac) is a widely distributed Gram-negative bacterium commonly found in moist habitats. This study investigates the feeding preferences, pharyngeal pumping rates, body fat content, lifespan, and gene expression differences of C. elegans when fed different bacterial food sources. The results reveal that feeding on Ac induces lipid metabolism changes, leading to altered feeding preferences. Transcriptomic analysis was performed to compare the gene expression profiles of C. elegans fed on Escherichia coli OP50 and Ac, offers a novel perspective on the mechanisms underlying C. elegans microbe interactions.

Key words: C. elegans, Acinetobacter calcoaceticus, food preferences, transcriptome

刘姣姣, 卢心河, 殷红彦, 周海龙, 李汉增, 徐顺清. 基于转录组学分析秀丽线虫对乙酸钙不动杆菌的摄食偏好行为[J]. 遗传, 2025, 47(7): 786-796.

Jiaojiao Liu, Xinhe Lu, Hongyan Yin, Hailong Zhou, Hanzeng Li, Shunqing Xu. Analysis of food preference behavior of C. elegans to Acinetobacter calcoaceticus based on transcriptomics[J]. Hereditas(Beijing), 2025, 47(7): 786-796.

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基于转录组学分析秀丽线虫对乙酸钙不动杆菌的摄食偏好行为

Analysis of food preference behavior of C. elegans to Acinetobacter calcoaceticus based on transcriptomics

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