高通量转录组测序技术在植物雄性不育研究中的应用

doi:10.16288/j.yczz.16-031

遗传 ›› 2016, Vol. 38 ›› Issue (8): 677-687.doi: 10.16288/j.yczz.16-031

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高通量转录组测序技术在植物雄性不育研究中的应用

刘永明, 张玲, 邱涛, 赵卓凡, 曹墨菊

四川农业大学玉米研究所,成都 611130

收稿日期:2016-01-18 修回日期:2016-04-13 出版日期:2016-08-20 发布日期:2016-06-01
通讯作者: 曹墨菊,教授,博士生导师,研究方向：玉米雄性不育。E-mail: caomj@sicau.edu.cn E-mail:liuluckforever@163.com
作者简介:刘永明,博士研究生,专业方向：玉米生物技术育种。E-mail: liuluckforever@163.com
基金资助:
四川省科技厅支撑计划项目(编号：2015NZ0001)资助

Research progress on mechanisms of male sterility in plants based on high-throughput RNA sequencing

Yongming Liu, Ling Zhang, Tao Qiu, Zhuofan Zhao, Moju Cao

Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-01-18 Revised:2016-04-13 Online:2016-08-20 Published:2016-06-01
Supported by:
Supported by the Science and Technology Department of Sichuan Province (No; 2015NZ0001)

摘要/Abstract

摘要： 植物雄性不育是指植物雄蕊发育受阻不能产生正常有功能花粉的现象。植物雄性不育不仅是生殖生理研究的宝贵材料,也是植物杂种优势利用的重要工具。由于高通量转录组测序技术几乎可以检测细胞内所有mRNA及非编码RNA的信息,已被广泛应用于生命科学研究的各项领域。在植物雄性不育相关研究中,高通量转录组测序技术在不同物种、不同败育类型中的应用已有报道,这为研究者在转录组水平综合了解植物雄性不育的分子机制及代谢网络提供了帮助。本文从测序文库构建策略、差异表达基因、非编码RNA的功能特征等方面综述了高通量转录组测序在植物雄性不育机理方面的研究进展,并探讨了转录组测序技术在花粉败育机制解析及育性相关基因定位中的应用价值,以期为植物雄性不育的相关研究提供参考。

关键词: 植物雄性不育, 转录组, 转录组测序, 非编码RNA

Abstract: Male sterility is defined as failing to produce functional pollen during stamen development in plants, and it plays a crucial role in plant reproductive research and hybrid seed production in utilization of crop heterosis. High throughput RNA sequencing (RNA-seq) has been used widely in the study of different fields of life science, as it readily detects all the mRNA and non-coding RNA in cells. Recently, RNA-seq has been reported to be applied in different species and kinds of pollen abortion types in plants, which has contributed to the understanding of the molecular mechanism and metabolic networks of male sterility at the transcription level. In this review, we summarize research progress on the mechanisms of male sterility in plants, focusing on RNA-seq analysis encompassing strategies of RNA library construction, differentially expressed genes and functional characteristics of noncoding RNAs involved in stamen abortion. Furthermore, we also discuss application of transcriptome sequencing technology to elucidate pollen abortion mechanisms and map fertility-related genes. We hope to provide references to the study of male sterility in plants.

Key words: plant male sterility, transcriptome, RNA sequencing, noncoding RNA

刘永明, 张玲, 邱涛, 赵卓凡, 曹墨菊. 高通量转录组测序技术在植物雄性不育研究中的应用[J]. 遗传, 2016, 38(8): 677-687.

Yongming Liu, Ling Zhang, Tao Qiu, Zhuofan Zhao, Moju Cao. Research progress on mechanisms of male sterility in plants based on high-throughput RNA sequencing[J]. Hereditas(Beijing), 2016, 38(8): 677-687.

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高通量转录组测序技术在植物雄性不育研究中的应用

Research progress on mechanisms of male sterility in plants based on high-throughput RNA sequencing

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