关键词: 印迹基因H19, DNA甲基化, ES小鼠, ES细胞

Abstract:

To investigate the relationship between the methylation status of imprinted gene H19 and development of mice derived completely from Embryonic stem cells (ES) by tetraploid embryo complementation. The methylation status of two loci at 5′UTR region in imprinted gene H19 in normal adult control mice, 22 adult ES mice, 8 newborn dead ES mice and embryonic stem (ES) cells with different passage number were detected by the using of methylation-sensitive restriction endonuclease-PCR technique. The results indicated that the methylation status of imprinted gene H19 in adult ES mice were identical to that of normal adult control mice. However, some significant differences in the methylation status of imprinted gene H19 were found among newborn dead ES mice, adult ES mice and normal adult control mice. Furthermore, the me-thylation status of imprinted gene H19 in ES cells were probably different from that of adult ES mice and normal adult con-trol mice.