遗传 ›› 2012, Vol. 34 ›› Issue (8): 1009-1019.doi: 10.3724/SP.J.1005.2012.01009
许红恩1, 张化浩1, 韩民锦1, 沈以红1, 黄先智1, 向仲怀1, 张泽1,2
收稿日期:2012-02-14
修回日期:2012-04-01
出版日期:2012-08-20
发布日期:2012-08-25
通讯作者:
张泽
E-mail:zezhang@swu.edu.cn
基金资助:西南大学研究生科技创新基金项目(优博项目)(编号：kb2010106)资助
XU Hong-En1, ZHANG Hua-Hao1, HAN Min-Jin1, SHEN Yi-Hong1, HUANG Xian-Zhi1, XIANG Zhong-Huai1, ZHANG Ze1, 2
Received:2012-02-14
Revised:2012-04-01
Online:2012-08-20
Published:2012-08-25
摘要： 重复序列是真核生物基因组的重要组成成分, 根据其序列特征及在基因组中的存在形式, 可以进一步分为串联重复、片段重复和散在重复。其中, 散在重复大多起源于转座子。根据转座介质的不同, 转座子又可分为DNA和逆转录转座子。转座子的转座和扩增对基因的进化和基因组的稳定具有显著的影响; 同时与其他类型的重复序列相比, 转座子的结构和分类更为复杂多样, 使得对转座子的鉴定和分类更为复杂和困难。鉴于此, 文章简要概括了转座子的功能及分类, 总结了真核生物转座子鉴定、分类和注释的3个步骤：(1)重复序列库的构建; (2)重复序列的校正和分类; (3)基因组注释。着重介绍了每一步骤所采用的不同计算方法, 比较了不同方法的优缺点。只有把多种方法结合起来使用才能实现全基因组转座子的精确鉴定、分类和注释, 这将为转座子的全基因组鉴定和分类提供借鉴意义。
许红恩，张化浩，韩民锦，沈以红，黄先智，向仲怀，张泽. 真核生物转座子鉴定和分类计算方法[J]. 遗传, 2012, 34(8): 1009-1019.
XU Hong-En, ZHANG Hua-Hao, HAN Min-Jin, SHEN Yi-Hong, HUANG Xian-Zhi, XIANG Zhong-Huai, ZHANG Ze. Computational approaches for identification and classification of transposable elements in eukaryotic genomes[J]. HEREDITAS, 2012, 34(8): 1009-1019.
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